AXR1-ECR1 and AXL1-ECR1 heterodimeric RUB-activating enzymes diverge in function in Arabidopsis thaliana

Hotton, Sara K.; Eigenheer, Richard A.; Castro, Meliza F.; Bostick, Magnolia; Callis, Judy

doi:10.1007/s11103-011-9750-8

AXR1-ECR1 and AXL1-ECR1 heterodimeric RUB-activating enzymes diverge in function in Arabidopsis thaliana

Journal Article · Thu Feb 10 23:00:00 EST 2011 · Plant Molecular Biology

DOI:https://doi.org/10.1007/s11103-011-9750-8· OSTI ID:1816645

Hotton, Sara K. ^[1]; Eigenheer, Richard A. ^[2]; Castro, Meliza F. ^[3]; Bostick, Magnolia ^[3]; Callis, Judy ^[3]

Univ. of California, Davis, CA (United States). Dept. of Molecular and Cellular Biology; OSTI
Univ. of California, Davis, CA (United States). Genome Center. Proteomics Core Facility
Univ. of California, Davis, CA (United States). Dept. of Molecular and Cellular Biology

Related to UBIQUITIN (RUB) modification of CULLIN (CUL) subunits of the CUL-RING ubiquitin E3 ligase (CRL) superfamily regulates CRL ubiquitylation activity. RUB modification requires E1 and E2 enzymes that are analogous to, but distinct from, those activities required for UBIQUITIN (UBQ) attachment. Gene duplications are widespread in angiosperms, and in line with this observation, components of the RUB conjugation pathway are found in multiples in Arabidopsis. To further examine the extent of redundancy within the RUB pathway, we undertook biochemical and genetic characterizations of one such duplication event- the duplication of the genes encoding a subunit of the RUB E1 into AUXIN RESISTANT1 (AXR1) and AXR1- LIKE1 (AXL1). In vitro, the two proteins have similar abilities to function with E1 C-TERMINAL-RELATED1 (ECR1) in catalyzing RUB1 activation and RUB1-ECR1 thioester formation. Using mass spectrometry, endogenous AXR1 and AXL1 proteins were found in complex with 3HARUB1, suggesting that AXR1 and AXL1 exist in parallel RUB E1 complexes in Arabidopsis. In contrast, AXR1 and AXL1 differ in ability to correct phenotypic defects in axr1- 30, a severe loss-of-function AXR1 mutant, when the respective coding sequences are expressed from the same promoter, suggesting differential in vivo functions. These results suggest that while both proteins function in the RUB pathway and are biochemically similar in RUB-ECR1 thioester formation, they are not functionally equivalent.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: FG02-03ER15416

OSTI ID:: 1816645

Journal Information:: Plant Molecular Biology, Journal Name: Plant Molecular Biology Journal Issue: 4-5 Vol. 75; ISSN 0167-4412

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
AUXIN RESISTANT1
AXR1-LIKE1
Arabidopsis thaliana
Functional divergence
RELATED TO UBIQUITIN
RUB-activating enzyme

AXR1-ECR1 and AXL1-ECR1 heterodimeric RUB-activating enzymes diverge in function in Arabidopsis thaliana

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References (49)

Cited By (2)

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