Use of a non-homologous end-joining-deficient strain (delta-ku70) of the biocontrol fungus Trichoderma virens to investigate the function of the laccase gene lcc1 in sclerotia degradation

Catalano, Valentina; Vergara, Mariarosaria; Hauzenberger, Jasmin R.; Seiboth, Bernhard; Sarrocco, Sabrina; Vannacci, Giovanni; Kubicek, Christian P.; Seidl-Seiboth, Verena

doi:10.1007/s00294-010-0322-2

Use of a non-homologous end-joining-deficient strain (delta-ku70) of the biocontrol fungus Trichoderma virens to investigate the function of the laccase gene lcc1 in sclerotia degradation

Journal Article · Sun Sep 26 00:00:00 EDT 2010 · Current Genetics

DOI:https://doi.org/10.1007/s00294-010-0322-2· OSTI ID:1816636

Catalano, Valentina ^[1]; Vergara, Mariarosaria ^[2]; Hauzenberger, Jasmin R. ^[3]; Seiboth, Bernhard ^[3]; Sarrocco, Sabrina ^[4]; Vannacci, Giovanni ^[4]; Kubicek, Christian P. ^[3]; Seidl-Seiboth, Verena ^[3]

Univ. of Pisa (Italy). Faculty of Agriculture. Plant Pathology Section. Dept. of Tree Science, Entomology and Plant Pathology; Vienna Univ. of Technology (Austria). Inst. of Chemical Engineering. Research Area Gene Technology and Applied Biochemistry; OSTI
Scuola Normale Superiore di Pisa (Italy)
Vienna Univ. of Technology (Austria). Inst. of Chemical Engineering. Research Area Gene Technology and Applied Biochemistry
Univ. of Pisa (Italy). Faculty of Agriculture. Plant Pathology Section. Dept. of Tree Science, Entomology and Plant Pathology

The aim of this study was to apply a generated Dtku70 strain with increased homologous recombination efficiency from the mycoparasitic fungus Trichoderma virens for studying the involvement of laccases in the degradation of sclerotia of plant pathogenic fungi. Inactivation of the non-homologous end-joining pathway has become a successful tool in filamentous fungi to overcome poor targeting efficiencies for genetic engineering. Here, we applied this principle to the biocontrol fungus T. virens, strain I10, by deleting its tku70 gene. This strain was subsequently used to delete the laccase gene lcc1, which we found to be expressed after interaction of T. virens with sclerotia of the plant pathogenic fungi Botrytis cinerea and Sclerotinia sclerotiorum. Lcc1 was strongly upregulated at early colonization of B. cinerea sclerotia and steadily induced during colonization of S. sclerotiorum sclerotia. The Dtku70Dlcc1 mutant was altered in its ability to degrade the sclerotia of B. cinerea and S. sclerotiorum. Interestingly, while the decaying ability for B. cinerea sclerotia was significantly decreased, that to degrade S. sclerotiorum sclerotia was even enhanced, suggesting the operation of different mechanisms in the mycoparasitism of these two types of sclerotia by the laccase LCC1.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: USDOE Office of Science (SC); Austrian Science Fund

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1816636

Journal Information:: Current Genetics, Journal Name: Current Genetics Journal Issue: 1 Vol. 57; ISSN 0172-8083

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Gene targeting
Laccase
Mycoparasitism
Sclerotia
Trichoderma virens
ku70

Use of a non-homologous end-joining-deficient strain (delta-ku70) of the biocontrol fungus Trichoderma virens to investigate the function of the laccase gene lcc1 in sclerotia degradation

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References (47)

Cited By (16)

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