Structural insights into photoactivation of plant Cryptochrome-2

Palayam, Malathy; Ganapathy, Jagadeesan; Guercio, Angelica M.; Tal, Lior; Deck, Samuel L.; Shabek, Nitzan

doi:10.1038/s42003-020-01531-x

Structural insights into photoactivation of plant Cryptochrome-2

Journal Article · Mon Jan 04 00:00:00 EST 2021 · Communications Biology

DOI:https://doi.org/10.1038/s42003-020-01531-x· OSTI ID:1816466

Palayam, Malathy ^[1]; Ganapathy, Jagadeesan ^[2]; Guercio, Angelica M. ^[2]; ^[2]; Deck, Samuel L. ^[2]; ^[2]

Univ. of California, Davis, CA (United States). Dept. of Plant Biology; OSTI
Univ. of California, Davis, CA (United States). Dept. of Plant Biology

Cryptochromes (CRYs) are evolutionarily conserved photoreceptors that mediate various light-induced responses in bacteria, plants, and animals. Plant cryptochromes govern a variety of critical growth and developmental processes including seed germination, flowering time and entrainment of the circadian clock. CRY’s photocycle involves reduction of their flavin adenine dinucleotide (FAD)-bound chromophore, which is completely oxidized in the dark and semi to fully reduced in the light signaling-active state. Despite the progress in characterizing cryptochromes, important aspects of their photochemistry, regulation, and light-induced structural changes remain to be addressed. In this study, we determine the crystal structure of the photosensory domain of Arabidopsis CRY2 in a tetrameric active state. Systematic structure-based analyses of photo-activated and inactive plant CRYs elucidate distinct structural elements and critical residues that dynamically partake in photo-induced oligomerization. Our study offers an updated model of CRYs photoactivation mechanism as well as the mode of its regulation by interacting proteins.

View Accepted Manuscript (DOE)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1816466

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 4; ISSN 2399-3642

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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