PTBP1 mRNA isoforms and regulation of their translation

Arake de Tacca, Luisa M.; Pulos-Holmes, Mia C.; Floor, Stephen N.; Cate, Jamie H.D.

doi:10.1261/rna.070193.118

PTBP1 mRNA isoforms and regulation of their translation

Journal Article · Mon Jul 01 00:00:00 EDT 2019 · RNA

DOI:https://doi.org/10.1261/rna.070193.118· OSTI ID:1816126

Arake de Tacca, Luisa M. ^[1]; Pulos-Holmes, Mia C. ^[2]; Floor, Stephen N. ^[3]; Cate, Jamie H.D. ^[4]

Univ. of California, Berkeley, CA (United States); OSTI
Univ. of California, Berkeley, CA (United States)
Univ. of California, San Francisco, CA (United States)
Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Polypyrimidine tract-binding proteins (PTBPs) are RNA binding proteins that regulate a number of posttranscriptional events. Human PTBP1 transits between the nucleus and cytoplasm and is thought to regulate RNA processes in both. However, information about PTBP1 mRNA isoforms and regulation of PTPB1 expression remains incomplete. Here we mapped the major PTBP1 mRNA isoforms in HEK293T cells and identified alternative 5' and 3' untranslated regions (5'-UTRs, 3'-UTRs), as well as alternative splicing patterns in the protein coding region. We also assessed how the observed PTBP1 mRNA isoforms contribute to PTBP1 expression in different phases of the cell cycle. Previously, PTBP1 mRNAs were shown to crosslink to eukaryotic translation initiation factor 3 (eIF3). We find that eIF3 binds differently to each PTBP1 mRNA isoform in a cell cycle dependent manner. We also observe a strong correlation between eIF3 binding to PTBP1 mRNAs and repression of PTBP1 levels during the S phase of the cell cycle. Our results provide evidence of translational regulation of PTBP1 protein levels during the cell cycle, which may affect downstream regulation of alternative splicing and translation mediated by PTBP1 protein isoforms.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Berkeley, CA (United States)

Sponsoring Organization:: CAPES Sciences Without Borders; National Institutes of Health (NIH); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1816126

Journal Information:: RNA, Journal Name: RNA Journal Issue: 10 Vol. 25; ISSN 1355-8382

Publisher:: Cold Spring Harbor Laboratory PressCopyright Statement

Country of Publication:: United States

Language:: English

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PTBP1 mRNA isoforms and regulation of their translation

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