Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Two Alternative Splicing Variants of AtERF73/HRE1, HRE1α and HRE1β, Have Differential Transactivation Activities in Arabidopsis

Journal Article · · International Journal of Molecular Sciences (Online)
DOI:https://doi.org/10.3390/ijms21196984· OSTI ID:1816087
 [1];  [2];  [3];  [2];  [4]
  1. Pusan National Univ., Busan (Korea, Republic of). Inst. of Systems Biology; OSTI
  2. Pusan National Univ., Busan (Korea, Republic of). Dept. of Integrated Biological Science
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division
  4. Pusan National Univ., Busan (Korea, Republic of). Inst. of Systems Biology; Pusan National Univ., Busan (Korea, Republic of). Dept. of Integrated Biological Science; Pusan National Univ., Busan (Korea, Republic of). Dept. of Molecular Biology
+ Show Author Affiliations
AtERF73/HRE1 is an AP2/ERF transcription factor in Arabidopsis and has two distinct alternative splicing variants, HRE1α and HRE1β. In this study, we examined the differences between the molecular functions of HRE1α and HRE1β. We found that HRE1α and HRE1β are both involved in hypoxia response and root development and have transactivation activity. Two conserved motifs in the C-terminal region of HRE1α and HRE1β, EELL and LWSY-like, contributed to their transactivation activity, specifically the four E residues in the EELL motif and the MGLWS amino acid sequence at the end of the LWSY-like motif. The N-terminal region of HRE1β also showed transactivation activity, mediated by the VDDG motif, whereas that of HRE1α did not. The transactivation activity of HRE1β was stronger than that of HRE1α in Arabidopsis protoplasts. Both transcription factors transactivated downstream genes via the GCC box. RNA-sequencing analysis further supported that both HRE1α and HRE1β might regulate gene expression associated with the hypoxia stress response, although they may transactivate different subsets of genes in downstream pathways. Our results, together with previous studies, suggested that HRE1α and HRE1β differentially transactivate downstream genes in hypoxia response and root development in Arabidopsis.
Research Organization:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1816087
Journal Information:
International Journal of Molecular Sciences (Online), Journal Name: International Journal of Molecular Sciences (Online) Journal Issue: 19 Vol. 21; ISSN IJMCFK; ISSN 1422-0067
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

References (54)

DNA-Binding Specificity of the ERF/AP2 Domain of Arabidopsis DREBs, Transcription Factors Involved in Dehydration- and Cold-Inducible Gene Expression journal January 2002
Plant homeodomain-leucine zipper I transcription factors exhibit different functional AHA motifs that selectively interact with TBP or/and TFIIB journal February 2014
Arabidopsis HRE1α, a splicing variant of AtERF73/HRE1, functions as a nuclear transcription activator in hypoxia response and root development journal April 2014
Plant NAC-type transcription factor proteins contain a NARD domain for repression of transcriptional activation journal August 2010
Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes journal January 2010
Cis-regulatory elements used to control gene expression in plants journal August 2016
Genome-wide analysis of AP2/ERF transcription factors family in Brassica napus journal June 2020
AP2/ERF family transcription factors in plant abiotic stress responses journal February 2012
Overexpression of Arabidopsis ZEP enhances tolerance to osmotic stress journal October 2008
The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box journal January 2006
Constitutively expressed ERF-VII transcription factors redundantly activate the core anaerobic response in Arabidopsis thaliana journal July 2015
Cold-inducible expression of an Arabidopsis thaliana AP2 transcription factor gene, AtCRAP2, promotes flowering under unsuitable low-temperatures in chrysanthemum journal January 2020
Homing into the origin of the AP2 DNA binding domain journal February 2005
Alternative Splicing Control of Abiotic Stress Responses journal February 2018
Expansion of the eukaryotic proteome by alternative splicing journal January 2010
Homeostatic response to hypoxia is regulated by the N-end rule pathway in plants journal October 2011
Fast gapped-read alignment with Bowtie 2 journal March 2012
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis journal June 2007
Understanding alternative splicing: towards a cellular code journal May 2005
Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor journal July 2011
Structural D/E-rich repeats play multiple roles especially in gene regulation through DNA/RNA mimicry journal January 2015
OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression journal February 2003
Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis journal June 2003
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana journal December 1998
Translational dynamics revealed by genome-wide profiling of ribosome footprints in Arabidopsis journal December 2013
BEDTools: a flexible suite of utilities for comparing genomic features journal January 2010
Storage of competent cells for Agrobacterium transformation journal January 1988
A Novel Group of Transcriptional Repressors in Arabidopsis journal March 2009
Gene Structures, Classification and Expression Models of the AP2/EREBP Transcription Factor Family in Rice journal December 2010
StuA is required for cell pattern formation in Aspergillus. journal September 1992
Genome-Wide Analysis of the ERF Gene Family in Arabidopsis and Rice journal January 2006
An Autoregulatory Loop Controlling Arabidopsis HsfA2 Expression: Role of Heat Shock-Induced Alternative Splicing journal March 2013
Group VII Ethylene Response Factors Coordinate Oxygen and Nitric Oxide Signal Transduction and Stress Responses in Plants journal May 2015
Transcriptomes of eight Arabidopsis thaliana accessions reveal core conserved, genotype- and organ-specific responses to flooding stress journal May 2016
Arabidopsis bZIP60 Is a Proteolysis-Activated Transcription Factor Involved in the Endoplasmic Reticulum Stress Response journal November 2008
A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis journal March 2013
Redundant ERF-VII Transcription Factors Bind to an Evolutionarily Conserved cis -Motif to Regulate Hypoxia-Responsive Gene Expression in Arabidopsis journal December 2015
Ethylene-inducible DNA binding proteins that interact with an ethylene-responsive element. journal February 1995
AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis journal September 2008
HRE1 and HRE2, two hypoxia-inducible ethylene response factors, affect anaerobic responses in Arabidopsis thaliana: HRE1 and HRE2 affect anaerobic responses in Arabidopsis thaliana journal January 2010
The EDLL motif: a potent plant transcriptional activation domain from AP2/ERF transcription factors: Strong plant transcriptional activation domain journal March 2012
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures journal July 1962
Flood adaptive traits and processes: an overview journal January 2015
High temperature attenuates the gravitropism of inflorescence stems by inducing SHOOT GRAVITROPISM 5 alternative splicing in A rabidopsis journal August 2015
DREB/CBF expression in wheat and barley using the stress‐inducible promoters of HD‐Zip I genes: impact on plant development, stress tolerance and yield journal September 2019
OXYGEN DEFICIENCY AND ROOT METABOLISM: Injury and Acclimation Under Hypoxia and Anoxia journal June 1997
Flooding Stress: Acclimations and Genetic Diversity journal June 2008
Genevestigator V3: A Reference Expression Database for the Meta-Analysis of Transcriptomes journal January 2008
Bioconductor: open software development for computational biology and bioinformatics journal September 2004
OsLIC, a Novel CCCH-Type Zinc Finger Protein with Transcription Activation, Mediates Rice Architecture via Brassinosteroids Signaling journal October 2008
The Role of Polypyrimidine Tract-Binding Proteins and Other hnRNP Proteins in Plant Splicing Regulation journal January 2012
Editorial: Alternative Splicing Regulation in Plants journal July 2020
Investigation of a Novel Salt Stress-Responsive Pathway Mediated by Arabidopsis DEAD-Box RNA Helicase Gene AtRH17 Using RNA-Seq Analysis journal February 2020
Frequentist Model Averaging and Applications to Bernoulli Trials journal January 2016

Cited By (1)

Additional file 1 of Genome-wide characterization and identification of candidate ERF genes involved in various abiotic stress responses in sesame (Sesamum indicum L.) dataset January 2022

Similar Records

The Small Ethylene Response Factor ERF96 is Involved in the Regulation of the Abscisic Acid Response in Arabidopsis
Journal Article · Wed Nov 25 19:00:00 EST 2015 · Frontiers in Plant Science · OSTI ID:1260689
Alternative splicing at NAGNAG acceptors in Arabidopsis thaliana SR and SR-related protein-coding genes
Journal Article · Wed Apr 09 20:00:00 EDT 2008 · BMC Genomics · OSTI ID:1626462

Related Subjects

36 MATERIALS SCIENCE
Arabidopsis
HRE1
alternative splicing
hypoxia
root development
transactivation