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The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription

Journal Article · · PLoS Pathogens
 [1];  [2];  [3];  [4];  [5];  [6];  [2]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; OSTI
  2. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrative Bio-Imaging Division; Univ. of California, Berkeley, CA (United States). Biophysics Graduate Group
  4. Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
  5. Univ. of California, Berkeley, CA (United States). School of Public Health. Division of Infectious Diseases and Immunity
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrative Bio-Imaging Division; Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology. California Institute for Quantitative Biosciences (QB3). Howard Hughes Medical Inst.
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β- and γ-herpesviruses include the oncogenic human viruses Kaposi’s sarcoma-associated virus (KSHV) and Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV), which is a significant cause of congenital disease. Near the end of their replication cycle, these viruses transcribe their late genes in a manner distinct from host transcription. Late gene transcription requires six virally encoded proteins, one of which is a functional mimic of host TATA-box-binding protein (TBP) that is also involved in recruitment of RNA polymerase II (Pol II) via unknown mechanisms. Here, we applied biochemical protein interaction studies together with electron microscopy-based imaging of a reconstituted human preinitiation complex to define the mechanism underlying Pol II recruitment. These data revealed that the herpesviral TBP, encoded by ORF24 in KSHV, makes a direct protein-protein contact with the C-terminal domain of host RNA polymerase II (Pol II), which is a unique feature that functionally distinguishes viral from cellular TBP. The interaction is mediated by the N-terminal domain (NTD) of ORF24 through a conserved motif that is shared in its β- and γ-herpesvirus homologs. Thus, these herpesviruses employ an unprecedented strategy in eukaryotic transcription, wherein promoter recognition and polymerase recruitment are facilitated by a single transcriptional activator with functionally distinct domains.
Research Organization:
Univ. of California, Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1815988
Journal Information:
PLoS Pathogens, Journal Name: PLoS Pathogens Journal Issue: 9 Vol. 16; ISSN 1553-7374
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

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