Biased localization of actin binding proteins by actin filament conformation

Harris, Andrew R.; Jreij, Pamela; Belardi, Brian; Joffe, Aaron M.; Bausch, Andreas R.; Fletcher, Daniel A.

doi:10.1038/s41467-020-19768-9

Biased localization of actin binding proteins by actin filament conformation

Journal Article · Tue Nov 24 23:00:00 EST 2020 · Nature Communications

DOI:https://doi.org/10.1038/s41467-020-19768-9· OSTI ID:1815971

Harris, Andrew R. ^[1]; Jreij, Pamela ^[2]; Belardi, Brian ^[2]; Joffe, Aaron M. ^[2]; Bausch, Andreas R. ^[3]; ^[4]

Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering and Biophysics Program; OSTI
Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering and Biophysics Program
Technische Universität München, Garching (Germany). Lehrstuhl für Biophysik (E27)
Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering and Biophysics Program; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Chan Zuckerberg Biohub, San Francisco, CA (United States)

The assembly of actin filaments into distinct cytoskeletal structures plays a critical role in cell physiology, but how proteins localize differentially to these structures within a shared cytoplasm remains unclear. Here, we show that the actin-binding domains of accessory proteins can be sensitive to filament conformational changes. Using a combination of live cell imaging and in vitro single molecule binding measurements, we show that tandem calponin homology domains (CH1–CH2) can be mutated to preferentially bind actin networks at the front or rear of motile cells. We demonstrate that the binding kinetics of CH1–CH2 domain mutants varies as actin filament conformation is altered by perturbations that include stabilizing drugs and other binding proteins. These findings suggest that conformational changes of actin filaments in cells could help to direct accessory binding proteins to different actin cytoskeletal structures through a biophysical feedback loop.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH); National Science Foundation (NSF); European Molecular Microbiology Organization (EMBO); Human Frontier Science Program (HFSP); Deutsche Forschungsgemeinschaft (DFG)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1815971

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 11; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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42 ENGINEERING
59 BASIC BIOLOGICAL SCIENCES
Actin
Cell migration
Kinetics
Single-molecule biophysics
Super-resolution microscopy

Biased localization of actin binding proteins by actin filament conformation

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