Two novel fish paralogs provide insights into the Rid family of imine deaminases active in pre-empting enamine/imine metabolic damage

Digiovanni, Stefania; Visentin, Cristina; Degani, Genny; Barbiroli, Alberto; Chiara, Matteo; Regazzoni, Luca; Di Pisa, Flavio; Borchert, Andrew J.; Downs, Diana M.; Ricagno, Stefano; Vanoni, Maria Antonietta; Popolo, Laura

doi:10.1038/s41598-020-66663-w

Title: Two novel fish paralogs provide insights into the Rid family of imine deaminases active in pre-empting enamine/imine metabolic damage

Journal Article · Tue Jun 23 00:00:00 EDT 2020 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-020-66663-w· OSTI ID:1815563

Digiovanni, Stefania ^[1]; Visentin, Cristina ^[2]; Degani, Genny ^[2]; Barbiroli, Alberto ^[3]; Chiara, Matteo ^[2]; Regazzoni, Luca ^[4]; Di Pisa, Flavio ^[2]; Borchert, Andrew J. ^[5]; Downs, Diana M. ^[6]; Ricagno, Stefano ^[2]; Vanoni, Maria Antonietta ^[2]; Popolo, Laura ^[2]

Univ. di Milano (Italy). Dept. of Biosciences; Univ. of Groningen (Netherlands). Dept. of Chemical Biology I
Univ. di Milano (Italy). Dept. of Biosciences
Univ. di Milano (Italy). Dept. of Food, Environmental and Nutritional Sciences
Univ. di Milano (Italy). Dept. of Pharmaceutical Sciences
Univ. of Georgia, Athens, GA (United States). Dept. of Microbiology; National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
Univ. of Georgia, Athens, GA (United States). Dept. of Microbiology

Reactive Intermediate Deaminase (Rid) protein superfamily includes eight families among which the RidA is conserved in all domains of life. RidA proteins accelerate the deamination of the reactive 2-aminoacrylate (2AA), an enamine produced by some pyridoxal phosphate (PLP)-dependent enzymes. 2AA accumulation inhibits target enzymes with a detrimental impact on fitness. As a consequence of whole genome duplication, teleost fish have two ridA paralogs, while other extant vertebrates contain a single-copy gene. We investigated the biochemical properties of the products of two paralogs, identified in Salmo salar. _SsRidA-1 and _SsRidA-2 complemented the growth defect of a Salmonella enterica ridA mutant, an in vivo model of 2AA stress. In vitro, both proteins hydrolyzed 2-imino acids (IA) to keto-acids and ammonia. _SsRidA-1 was active on IA derived from nonpolar amino acids and poorly active or inactive on IA derived from other amino acids tested. In contrast, SsRidA-2 had a generally low catalytic efficiency, but showed a relatively higher activity with IA derived from L-Glu and aromatic amino acids. The crystal structures of _SsRidA-1 and _SsRidA-2 provided hints of the remarkably different conformational stability and substrate specificity. Overall, _SsRidA-1 is similar to the mammalian orthologs whereas SsRidA-2 displays unique properties likely generated by functional specialization of a duplicated ancestral gene.

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Research Organization:: Alliance for Sustainable Energy, LLC (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1815563

Journal Information:: Scientific Reports, Vol. 10, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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