Microbiome Aggregated Traits and Assembly Are More Sensitive to Soil Management than Diversity

Neal, Andrew L.; Hughes, David; Clark, Ian M.; Jansson, Janet K.; Hirsch, Penny R.

doi:10.1128/msystems.01056-20

Microbiome Aggregated Traits and Assembly Are More Sensitive to Soil Management than Diversity

Journal Article · Thu May 27 00:00:00 EDT 2021 · mSystems

DOI:https://doi.org/10.1128/msystems.01056-20· OSTI ID:1813588

^[1]; Hughes, David ^[2]; Clark, Ian M. ^[2]; Jansson, Janet K. ^[3]; Hirsch, Penny R. ^[2]

Rothamsted Research, Devon (United Kingdom)
Rothamsted Research, Hertfordshire (United Kingdom)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

How soil is managed, particularly for agriculture, exerts stresses upon soil microbiomes, resulting in altered community structures and functional states. Understanding how soil microbiomes respond to combined stresses is important for predicting system performance under different land use scenarios, aids in identification of the most environmentally benign managements, and provides insight into how system function can be recovered in degraded soils. We use a long-established field experiment to study the effects of combined chronic (press) disturbance of the magnitude of organic carbon inputs with acute (pulse) effects of physical disturbance by tillage and chemical disturbance due to inorganic fertilization and pesticide application. We show that because of the variety of ways it can be assessed, biodiversity—here based on microbial small subunit rRNA gene phylotypes—does not provide a consistent view of community change. In contrast, aggregated traits associated with soil microbiomes indicate general loss of function, measured as a reduction of average genome lengths, associated with chronic reduction of organic inputs in arable or bare fallow soils and altered growth strategies associated with rRNA operon copy number in prokaryotes, as well as a switch to pathogenicity in fungal communities. In addition, pulse disturbance by soil tillage is associated with an increased influence of stochastic processes upon prokaryote community assembly, but fungicide used in arable soils results in niche assembly of fungal communities compared to untilled grassland. Overall, bacteria, archaea, and fungi do not share a common response to land management change, and estimates of biodiversity do not capture important facets of community adaptation to stresses adequately.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: BBSRC; USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1813588

Report Number(s):: PNNL-SA--159143

Journal Information:: mSystems, Journal Name: mSystems Journal Issue: 3 Vol. 6; ISSN 2379-5077

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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