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Identification of a Na+-Binding Site near the Oxygen-Evolving Complex of Spinach Photosystem II

Journal Article · · Biochemistry
 [1];  [2];  [2];  [2];  [2];  [2];  [3];  [2];  [2];  [3];  [2];  [2]
  1. Yale Univ., New Haven, CT (United States); Yale University
  2. Yale Univ., New Haven, CT (United States)
  3. City College of New York, NY (United States); The Graduate Center of the City Univ. of New York, NY (United States)
+ Show Author Affiliations
The oxygen-evolving complex (OEC) of photosystem II (PSII) is an oxomanganese cluster composed of four redox-active Mn ions, and one redox-inactive Ca2+ ion, with two nearby bound Cl– ions. Sodium is a common counter-ion of both chloride and hydroxide anions, and a sodium-specific binding site has not been identified near the OEC. Furthermore, we find that the oxygen-evolution activity of spinach PSII increases with [Na+], particularly at high pH. A Na+-specific binding site next to the OEC, upon deprotonation of the D1-H337 amino-acid residue, is suggested by the analysis of two recently published PSII cryo-EM maps in combination with quantum mechanical calculations and molecular dynamics simulations.
Research Organization:
Yale Univ., New Haven, CT (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division
Grant/Contract Number:
FG02-05ER15646; SC0001423
OSTI ID:
1812292
Alternate ID(s):
OSTI ID: 1800236
Journal Information:
Biochemistry, Journal Name: Biochemistry Journal Issue: 30 Vol. 59; ISSN 0006-2960
Publisher:
American Chemical Society (ACS)Copyright Statement
Country of Publication:
United States
Language:
English

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
Crystal structure
Ions
Ligands
Molecules
Reaction mechanisms