Anchor extension: a structure-guided approach to design cyclic peptides targeting enzyme active sites

Hosseinzadeh, Parisa; Watson, Paris R.; Craven, Timothy W.; Li, Xinting; Rettie, Stephen; Pardo-Avila, Fátima; Bera, Asim K.; Mulligan, Vikram Khipple; Lu, Peilong; Ford, Alexander S.; Weitzner, Brian D.; Stewart, Lance J.; Moyer, Adam P.; Di Piazza, Maddalena; Whalen, Joshua G.; Greisen, Per; Christianson, David W.; Baker, David

doi:10.1038/s41467-021-23609-8

Anchor extension: a structure-guided approach to design cyclic peptides targeting enzyme active sites

Journal Article · Mon Jun 07 00:00:00 EDT 2021 · Nature Communications

DOI:https://doi.org/10.1038/s41467-021-23609-8· OSTI ID:1812055

^[1]; ^[2]; Craven, Timothy W. ^[1]; Li, Xinting ^[1]; Rettie, Stephen ^[3]; Pardo-Avila, Fátima ^[4]; Bera, Asim K. ^[1]; Mulligan, Vikram Khipple ^[1]; ^[1]; Ford, Alexander S. ^[1]; ^[1]; ^[1]; ^[5]; Di Piazza, Maddalena ^[1]; Whalen, Joshua G. ^[1]; Greisen, Per ^[1]; ^[2]; ^[1]

Univ. of Washington, Seattle, WA (United States). Dept. of Biochemistry. Inst. for Protein Design
Univ. of Pennsylvania, Philadelphia, PA (United States). Roy and Diana Vagelos Lab. Dept. of Chemistry
Univ. of Washington, Seattle, WA (United States). Dept. of Biochemistry. Inst. for Protein Design. Molecular and Cellular Biology Ph.D. Program
Stanford Univ. School of Medicine, CA (United States). Dept. of Structural Biology
Univ. of Washington, Seattle, WA (United States). Dept. of Biochemistry. Inst. for Protein Design. Molecular Engineering Ph.D. Program

Despite recent success in computational design of structured cyclic peptides, de novo design of cyclic peptides that bind to any protein functional site remains difficult. To address this challenge, we develop a computational “anchor extension” methodology for targeting protein interfaces by extending a peptide chain around a non-canonical amino acid residue anchor. To test our approach using a well characterized model system, we design cyclic peptides that inhibit histone deacetylases 2 and 6 (HDAC2 and HDAC6) with enhanced potency compared to the original anchor (IC₅₀ values of 9.1 and 4.4 nM for the best binders compared to 5.4 and 0.6 µM for the anchor, respectively). The HDAC6 inhibitor is among the most potent reported so far. These results highlight the potential for de novo design of high-affinity protein-peptide interfaces, as well as the challenges that remain.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0012704

OSTI ID:: 1812055

Alternate ID(s):: OSTI ID: 1875646

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 12; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

References (76)

LINCS: A linear constraint solver for molecular simulations Hess, Berk; Bekker, Henk; Berendsen, Herman J. C. Journal of Computational Chemistry, Vol. 18, Issue 12 https://doi.org/10.1002/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H	journal	September 1997
Structure-Based Design of Inhibitors of Protein-Protein Interactions: Mimicking Peptide Binding Epitopes Pelay-Gimeno, Marta; Glas, Adrian; Koch, Oliver Angewandte Chemie International Edition, Vol. 54, Issue 31 https://doi.org/10.1002/anie.201412070	journal	June 2015
Improvements to the APBS biomolecular solvation software suite: Improvements to the APBS Software Suite Jurrus, Elizabeth; Engel, Dave; Star, Keith Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3280	journal	October 2017
MolProbity: More and better reference data for improved all-atom structure validation: PROTEIN SCIENCE.ORG Williams, Christopher J.; Headd, Jeffrey J.; Moriarty, Nigel W. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3330	journal	November 2017
Integration of the Rosetta suite with the python software stack via reproducible packaging and core programming interfaces for distributed simulation Ford, Alexander S.; Weitzner, Brian D.; Bahl, Christopher D. Protein Science, Vol. 29, Issue 1 https://doi.org/10.1002/pro.3721	journal	November 2019
DynaDock: A new molecular dynamics-based algorithm for protein-peptide docking including receptor flexibility Antes, Iris Proteins: Structure, Function, and Bioinformatics, Vol. 78, Issue 5 https://doi.org/10.1002/prot.22629	journal	October 2009
Improved side-chain torsion potentials for the Amber ff99SB protein force field Lindorff-Larsen, Kresten; Piana, Stefano; Palmo, Kim Proteins: Structure, Function, and Bioinformatics https://doi.org/10.1002/prot.22711	journal	January 2010
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
Just add water! The effect of water on the specificity of protein-ligand binding sites and its potential application to drug design Ladbury, John E. Chemistry & Biology, Vol. 3, Issue 12 https://doi.org/10.1016/S1074-5521(96)90164-7	journal	December 1996
Tumor-targeting peptides from combinatorial libraries Liu, Ruiwu; Li, Xiaocen; Xiao, Wenwu Advanced Drug Delivery Reviews, Vol. 110-111 https://doi.org/10.1016/j.addr.2016.05.009	journal	February 2017
Therapeutic peptides: Historical perspectives, current development trends, and future directions Lau, Jolene L.; Dunn, Michael K. Bioorganic & Medicinal Chemistry, Vol. 26, Issue 10 https://doi.org/10.1016/j.bmc.2017.06.052	journal	June 2018
Exploration of the HDAC2 foot pocket: Synthesis and SAR of substituted N-(2-aminophenyl)benzamides Bressi, Jerome C.; Jennings, Andy J.; Skene, Robert Bioorganic & Medicinal Chemistry Letters, Vol. 20, Issue 10 https://doi.org/10.1016/j.bmcl.2010.03.091	journal	May 2010
Cyclic peptide therapeutics: past, present and future Zorzi, Alessandro; Deyle, Kaycie; Heinis, Christian Current Opinion in Chemical Biology, Vol. 38 https://doi.org/10.1016/j.cbpa.2017.02.006	journal	June 2017
Engineered protein scaffolds as leads for synthetic inhibitors of protein–protein interactions Wuo, Michael G.; Arora, Paramjit S. Current Opinion in Chemical Biology, Vol. 44 https://doi.org/10.1016/j.cbpa.2018.05.013	journal	June 2018
Messenger RNA-Programmed Incorporation of Multiple N-Methyl-Amino Acids into Linear and Cyclic Peptides Kawakami, Takashi; Murakami, Hiroshi; Suga, Hiroaki Chemistry & Biology, Vol. 15, Issue 1 https://doi.org/10.1016/j.chembiol.2007.12.008	journal	January 2008
A flexible algorithm for calculating pair interactions on SIMD architectures Páll, Szilárd; Hess, Berk Computer Physics Communications, Vol. 184, Issue 12 https://doi.org/10.1016/j.cpc.2013.06.003	journal	December 2013
Computational Design of a Protein-Based Enzyme Inhibitor Procko, Erik; Hedman, Rickard; Hamilton, Keith Journal of Molecular Biology, Vol. 425, Issue 18 https://doi.org/10.1016/j.jmb.2013.06.035	journal	September 2013
Structure, mechanism, and inhibition of histone deacetylases and related metalloenzymes Lombardi, Patrick M.; Cole, Kathryn E.; Dowling, Daniel P. Current Opinion in Structural Biology, Vol. 21, Issue 6 https://doi.org/10.1016/j.sbi.2011.08.004	journal	December 2011
GROMACS: High performance molecular simulations through multi-level parallelism from laptops to supercomputers Abraham, Mark James; Murtola, Teemu; Schulz, Roland SoftwareX, Vol. 1-2 https://doi.org/10.1016/j.softx.2015.06.001	journal	September 2015
RNA Display Methods for the Discovery of Bioactive Macrocycles Huang, Yichao; Wiedmann, Mareike Margarete; Suga, Hiroaki Chemical Reviews, Vol. 119, Issue 17 https://doi.org/10.1021/acs.chemrev.8b00430	journal	November 2018
Crystal Structures of Protein-Bound Cyclic Peptides Malde, Alpeshkumar K.; Hill, Timothy A.; Iyer, Abishek Chemical Reviews, Vol. 119, Issue 17 https://doi.org/10.1021/acs.chemrev.8b00807	journal	May 2019
Docking of Macrocycles: Comparing Rigid and Flexible Docking in Glide Alogheli, Hiba; Olanders, Gustav; Schaal, Wesley Journal of Chemical Information and Modeling, Vol. 57, Issue 2 https://doi.org/10.1021/acs.jcim.6b00443	journal	February 2017
Peptidic Macrocycles - Conformational Sampling and Thermodynamic Characterization Kamenik, Anna S.; Lessel, Uta; Fuchs, Julian E. Journal of Chemical Information and Modeling, Vol. 58, Issue 5 https://doi.org/10.1021/acs.jcim.8b00097	journal	April 2018
The Current State of Peptide Drug Discovery: Back to the Future? Henninot, Antoine; Collins, James C.; Nuss, John M. Journal of Medicinal Chemistry, Vol. 61, Issue 4 https://doi.org/10.1021/acs.jmedchem.7b00318	journal	August 2017
Selective Targeting of Integrin αvβ8 by a Highly Active Cyclic Peptide Reichart, Florian; Maltsev, Oleg V.; Kapp, Tobias G. Journal of Medicinal Chemistry, Vol. 62, Issue 4 https://doi.org/10.1021/acs.jmedchem.8b01588	journal	January 2019
Identification and Optimization of EphA2-Selective Bicycles for the Delivery of Cytotoxic Payloads Mudd, Gemma E.; Brown, Amy; Chen, Liuhong Journal of Medicinal Chemistry, Vol. 63, Issue 8 https://doi.org/10.1021/acs.jmedchem.9b02129	journal	March 2020
Direct Peptide Cyclization and One-Pot Modification Using the MeDbz Linker Gless, Bengt H.; Olsen, Christian A. The Journal of Organic Chemistry, Vol. 83, Issue 17 https://doi.org/10.1021/acs.joc.8b01237	journal	July 2018
Binding of the Microbial Cyclic Tetrapeptide Trapoxin A to the Class I Histone Deacetylase HDAC8 Porter, Nicholas J.; Christianson, David W. ACS Chemical Biology, Vol. 12, Issue 9 https://doi.org/10.1021/acschembio.7b00330	journal	August 2017
A Hydrogen Bond Surrogate Approach for Stabilization of Short Peptide Sequences in α-Helical Conformation Patgiri, Anupam; Jochim, Andrea L.; Arora, Paramjit S. Accounts of Chemical Research, Vol. 41, Issue 10 https://doi.org/10.1021/ar700264k	journal	October 2008
Discovery of Light-Responsive Ligands through Screening of a Light-Responsive Genetically Encoded Library Jafari, Mohammad R.; Deng, Lu; Kitov, Pavel I. ACS Chemical Biology, Vol. 9, Issue 2 https://doi.org/10.1021/cb4006722	journal	November 2013
Thermodynamics of Phosphopeptide Tethering to BRCT: The Structural Minima for Inhibitor Design Lokesh, G. L.; Muralidhara, B. K.; Negi, Surendra S. Journal of the American Chemical Society, Vol. 129, Issue 35 https://doi.org/10.1021/ja0739178	journal	September 2007
Structural Basis of the Antiproliferative Activity of Largazole, a Depsipeptide Inhibitor of the Histone Deacetylases Cole, Kathryn E.; Dowling, Daniel P.; Boone, Matthew A. Journal of the American Chemical Society, Vol. 133, Issue 32 https://doi.org/10.1021/ja205972n	journal	August 2011
Thermodynamic and Structural Effects of Conformational Constraints in Protein−Ligand Interactions. Entropic Paradoxy Associated with Ligand Preorganization DeLorbe, John E.; Clements, John H.; Teresk, Martin G. Journal of the American Chemical Society, Vol. 131, Issue 46 https://doi.org/10.1021/ja904698q	journal	November 2009
Macrocyclic Modalities Combining Peptide Epitopes and Natural Product Fragments Guéret, Stéphanie M.; Thavam, Sasikala; Carbajo, Rodrigo J. Journal of the American Chemical Society, Vol. 142, Issue 10 https://doi.org/10.1021/jacs.0c00269	journal	February 2020
Analysis of Loops that Mediate Protein–Protein Interactions and Translation into Submicromolar Inhibitors Siegert, Timothy R.; Bird, Michael J.; Makwana, Kamlesh M. Journal of the American Chemical Society, Vol. 138, Issue 39 https://doi.org/10.1021/jacs.6b05656	journal	September 2016
Targeting Unoccupied Surfaces on Protein–Protein Interfaces Rooklin, David; Modell, Ashley E.; Li, Haotian Journal of the American Chemical Society, Vol. 139, Issue 44 https://doi.org/10.1021/jacs.7b05960	journal	August 2017
Display Selection of Exotic Macrocyclic Peptides Expressed under a Radically Reprogrammed 23 Amino Acid Genetic Code Passioura, Toby; Liu, Wenyu; Dunkelmann, Daniel Journal of the American Chemical Society, Vol. 140, Issue 37 https://doi.org/10.1021/jacs.8b03367	journal	August 2018
Cell-Permeable Bicyclic Peptidyl Inhibitors against NEMO-IκB Kinase Interaction Directly from a Combinatorial Library Rhodes, Curran A.; Dougherty, Patrick G.; Cooper, Jahan K. Journal of the American Chemical Society, Vol. 140, Issue 38 https://doi.org/10.1021/jacs.8b06738	journal	September 2018
Macrocyclic Peptides as Drug Candidates: Recent Progress and Remaining Challenges Vinogradov, Alexander A.; Yin, Yizhen; Suga, Hiroaki Journal of the American Chemical Society, Vol. 141, Issue 10 https://doi.org/10.1021/jacs.8b13178	journal	February 2019
Synthesis and Histone Deacetylase Inhibitory Activity of Largazole Analogs: Alteration of the Zinc-Binding Domain and Macrocyclic Scaffold Bowers, Albert A.; West, Nathan; Newkirk, Tenaya L. Organic Letters, Vol. 11, Issue 6 https://doi.org/10.1021/ol900078k	journal	February 2009
Accurate de novo design of hyperstable constrained peptides Bhardwaj, Gaurav; Mulligan, Vikram Khipple; Bahl, Christopher D. Nature, Vol. 538, Issue 7625 https://doi.org/10.1038/nature19791	journal	September 2016
Histone deacetylase 6 structure and molecular basis of catalysis and inhibition Hai, Yang; Christianson, David W. Nature Chemical Biology, Vol. 12, Issue 9 https://doi.org/10.1038/nchembio.2134	journal	July 2016
n→π* interactions in proteins Bartlett, Gail J.; Choudhary, Amit; Raines, Ronald T. Nature Chemical Biology, Vol. 6, Issue 8 https://doi.org/10.1038/nchembio.406	journal	July 2010
Insights into the activation mechanism of class I HDAC complexes by inositol phosphates Watson, Peter J.; Millard, Christopher J.; Riley, Andrew M. Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms11262	journal	April 2016
Highly selective inhibition of histone demethylases by de novo macrocyclic peptides Kawamura, Akane; Münzel, Martin; Kojima, Tatsuya Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14773	journal	April 2017
The many roles of histone deacetylases in development and physiology: implications for disease and therapy Haberland, Michael; Montgomery, Rusty L.; Olson, Eric N. Nature Reviews Genetics, Vol. 10, Issue 1 https://doi.org/10.1038/nrg2485	journal	January 2009
In-solution enrichment identifies peptide inhibitors of protein–protein interactions Touti, Fayçal; Gates, Zachary P.; Bandyopadhyay, Anupam Nature Chemical Biology, Vol. 15, Issue 4 https://doi.org/10.1038/s41589-019-0245-2	journal	March 2019
Dual-targeting anti-angiogenic cyclic peptides as potential drug leads for cancer therapy Chan, Lai Yue; Craik, David J.; Daly, Norelle L. Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep35347	journal	October 2016
Adapting free energy perturbation simulations for large macrocyclic ligands: how to dissect contributions from direct binding and free ligand flexibility Wallraven, Kerstin; Holmelin, Fredrik L.; Glas, Adrian Chemical Science, Vol. 11, Issue 8 https://doi.org/10.1039/C9SC04705K	journal	January 2020
Target-templated de novo design of macrocyclic d -/ l -peptides: discovery of drug-like inhibitors of PD-1 Guardiola, Salvador; Varese, Monica; Roig, Xavier Chemical Science, Vol. 12, Issue 14 https://doi.org/10.1039/D1SC01031J	journal	January 2021
Canonical sampling through velocity rescaling Bussi, Giovanni; Donadio, Davide; Parrinello, Michele The Journal of Chemical Physics, Vol. 126, Issue 1 https://doi.org/10.1063/1.2408420	journal	January 2007
Molecular dynamics with coupling to an external bath Berendsen, H. J. C.; Postma, J. P. M.; van Gunsteren, W. F. The Journal of Chemical Physics, Vol. 81, Issue 8 https://doi.org/10.1063/1.448118	journal	October 1984
A smooth particle mesh Ewald method Essmann, Ulrich; Perera, Lalith; Berkowitz, Max L. The Journal of Chemical Physics, Vol. 103, Issue 19 https://doi.org/10.1063/1.470117	journal	November 1995
Computationally designed peptide macrocycle inhibitors of New Delhi metallo-β-lactamase 1 Mulligan, Vikram Khipple; Workman, Sean; Sun, Tianjun Proceedings of the National Academy of Sciences, Vol. 118, Issue 12 https://doi.org/10.1073/pnas.2012800118	journal	March 2021
Bioactive Peptide Design Based on Protein Surface Epitopes Satoh, Takashi; Aramini, James M.; Li, Song Journal of Biological Chemistry, Vol. 272, Issue 18 https://doi.org/10.1074/jbc.272.18.12175	journal	May 1997
Peptiderive server: derive peptide inhibitors from protein–protein interactions Sedan, Yuval; Marcu, Orly; Lyskov, Sergey Nucleic Acids Research, Vol. 44, Issue W1 https://doi.org/10.1093/nar/gkw385	journal	May 2016
HDACs and HDAC Inhibitors in Cancer Development and Therapy Li, Yixuan; Seto, Edward Cold Spring Harbor Perspectives in Medicine, Vol. 6, Issue 10 https://doi.org/10.1101/cshperspect.a026831	journal	September 2016
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
iMOSFLM : a new graphical interface for diffraction-image processing with MOSFLM Battye, T. Geoff G.; Kontogiannis, Luke; Johnson, Owen Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910048675	journal	March 2011
How good are my data and what is the resolution? Evans, Philip R.; Murshudov, Garib N. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913000061	journal	June 2013
Potent peptidic fusion inhibitors of influenza virus Kadam, Rameshwar U.; Juraszek, Jarek; Brandenburg, Boerries Science, Vol. 358, Issue 6362 https://doi.org/10.1126/science.aan0516	journal	September 2017
Comprehensive computational design of ordered peptide macrocycles Hosseinzadeh, Parisa; Bhardwaj, Gaurav; Mulligan, Vikram Khipple Science, Vol. 358, Issue 6369 https://doi.org/10.1126/science.aap7577	journal	December 2017
Genetically Encoded Libraries of Nonstandard Peptides Kawakami, Takashi; Murakami, Hiroshi Journal of Nucleic Acids, Vol. 2012 https://doi.org/10.1155/2012/713510	journal	January 2012
Efficient conformational ensemble generation of protein-bound peptides Yan, Yumeng; Zhang, Di; Huang, Sheng-You Journal of Cheminformatics, Vol. 9, Issue 1 https://doi.org/10.1186/s13321-017-0246-7	journal	November 2017
Dual-targeting anti-angiogenic cyclic peptides as potential drug leads for cancer therapy Chan, Lai Yue; Craik, David J.; Daly, Norelle L. Biological Magnetic Resonance Bank https://doi.org/10.13018/bmr26882	dataset	January 2016
Bound Water at Protein-Protein Interfaces: Partners, Roles and Hydrophobic Bubbles as a Conserved Motif Ahmed, Mostafa H.; Spyrakis, Francesca; Cozzini, Pietro PLoS ONE, Vol. 6, Issue 9 https://doi.org/10.1371/journal.pone.0024712	journal	September 2011
Discovery and Mechanism of Natural Products as Modulators of Histone Acetylation A. Salvador, Lilibeth; Luesch, Hendrik Current Drug Targets, Vol. 13, Issue 8 https://doi.org/10.2174/138945012802008973	journal	June 2012
Computational Studies on the Histone Deacetylases and the Design of Selective Histone Deacetylase Inhibitors Wang, Difei Current Topics in Medicinal Chemistry, Vol. 9, Issue 3 https://doi.org/10.2174/156802609788085287	journal	February 2009
An Overview of Naturally Occurring Histone Deacetylase Inhibitors Kim, Bumki; Hong, Jiyong Current Topics in Medicinal Chemistry, Vol. 14, Issue 24 https://doi.org/10.2174/1568026615666141208105614	journal	January 2015
Use of Peptide Libraries for Identification and Optimization of Novel Antimicrobial Peptides Ashby, Martin; Petkova, Asya; Gani, Jurnorain Current Topics in Medicinal Chemistry, Vol. 17, Issue 5 https://doi.org/10.2174/1568026616666160713125555	journal	December 2016
Histone Deacetylase Inhibitors: An Attractive Therapeutic Strategy Against Breast Cancer Damaskos, Christos; Valsami, Serena; Kontos, Michael Anticancer Research, Vol. 37, Issue 1 https://doi.org/10.21873/anticanres.11286	journal	January 2017
Histone Deacetylase Inhibitors as Anticancer Drugs Eckschlager, Tomas; Plch, Johana; Stiborova, Marie International Journal of Molecular Sciences, Vol. 18, Issue 7 https://doi.org/10.3390/ijms18071414	journal	July 2017

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Anchor extension: a structure-guided approach to design cyclic peptides targeting enzyme active sites

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