Exploring Proteomes of Robust Yarrowia lipolytica Isolates Cultivated in Biomass Hydrolysate Reveals Key Processes Impacting Mixed Sugar Utilization, Lipid Accumulation, and Degradation

Walker, Caleb; Dien, Bruce; Giannone, Richard J.; Slininger, Patricia; Thompson, Stephanie R.; Trinh, Cong T.; Tullman-Ercek, ed., Danielle

doi:10.1128/mSystems.00443-21

Title: Exploring Proteomes of Robust Yarrowia lipolytica Isolates Cultivated in Biomass Hydrolysate Reveals Key Processes Impacting Mixed Sugar Utilization, Lipid Accumulation, and Degradation

Journal Article · Tue Aug 31 00:00:00 EDT 2021 · mSystems

DOI:https://doi.org/10.1128/mSystems.00443-21· OSTI ID:1811719

Walker, Caleb ^[1]; Dien, Bruce ^[2]; Giannone, Richard J. ^[3]; Slininger, Patricia ^[2]; Thompson, Stephanie R. ^[2];

^[1]; Tullman-Ercek, ed., Danielle

Department of Chemical and Biomolecular Engineering, University of Tennessee, Tennessee, USA
Bioenergy Research Unit, The National Center for Agricultural Utilization Research, USDA-ARS, Peoria, Illinois, USA
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

Yarrowia lipolytica is an oleaginous yeast exhibiting robust phenotypes beneficial for industrial biotechnology. The phenotypic diversity found within the undomesticated Y. lipolytica clade from various origins illuminates desirable phenotypic traits not found in the conventional laboratory strain CBS7504 (or W29), which include xylose utilization, lipid accumulation, and growth on undetoxified biomass hydrolysates. Currently, the related phenotypes of lipid accumulation and degradation when metabolizing nonpreferred sugars (e.g., xylose) associated with biomass hydrolysates are poorly understood, making it difficult to control and engineer in Y. lipolytica. To fill this knowledge gap, we analyzed the genetic diversity of five undomesticated Y. lipolytica strains and identified singleton genes and genes exclusively shared by strains exhibiting desirable phenotypes. Strain characterizations from controlled bioreactor cultures revealed that the undomesticated strain YB420 used xylose to support cell growth and maintained high lipid levels, while the conventional strain CBS7504 degraded cell biomass and lipids when xylose was the sole remaining carbon source. From proteomic analysis, we identified carbohydrate transporters, xylose metabolic enzymes, and pentose phosphate pathway proteins stimulated during the xylose uptake stage for both strains. Furthermore, we distinguished proteins involved in lipid metabolism (e.g., lipase, NADPH generation, lipid regulators, and b-oxidation) activated by YB420 (lipid maintenance phenotype) or CBS7504 (lipid degradation phenotype) when xylose was the sole remaining carbon source. Overall, the results relate genetic diversity of undomesticated Y. lipolytica strains to complex phenotypes of superior growth, sugar utilization, lipid accumulation, and degradation in biomass hydrolysates.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDA

Grant/Contract Number:: SC0019412; AC05-00OR22725; AC02-05CH11231

OSTI ID:: 1811719

Alternate ID(s):: OSTI ID: 1817416

Journal Information:: mSystems, Journal Name: mSystems Vol. 6 Journal Issue: 4; ISSN 2379-5077

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Exploring Proteomes of Robust Yarrowia lipolytica Isolates Cultivated in Biomass Hydrolysate Reveals Key Processes Impacting Mixed Sugar Utilization, Lipid Accumulation, and Degradation

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