Towards engineering ectomycorrhization into switchgrass bioenergy crops via a lectin receptor‐like kinase

Qiao, Zhenzhen; Yates, Timothy B.; Shrestha, Him K.; Engle, Nancy L.; Flanagan, Amy; Morrell‐Falvey, Jennifer L.; Sun, Yali; Tschaplinski, Timothy J.; Abraham, Paul E.; Labbé, Jessy; Wang, Zeng‐Yu; Hettich, Robert L.; Tuskan, Gerald A.; Muchero, Wellington; Chen, Jin‐Gui

doi:10.1111/pbi.13671

Towards engineering ectomycorrhization into switchgrass bioenergy crops via a lectin receptor‐like kinase

Journal Article · Wed Aug 11 00:00:00 EDT 2021 · Plant Biotechnology Journal

DOI:https://doi.org/10.1111/pbi.13671· OSTI ID:1812816

^[1]; Yates, Timothy B. ^[2]; Shrestha, Him K. ^[3]; Engle, Nancy L. ^[1]; Flanagan, Amy ^[4]; Morrell‐Falvey, Jennifer L. ^[1]; ^[1]; Tschaplinski, Timothy J. ^[1]; Abraham, Paul E. ^[5]; Labbé, Jessy ^[1]; Wang, Zeng‐Yu ^[4]; Hettich, Robert L. ^[5]; Tuskan, Gerald A. ^[1]; Muchero, Wellington ^[1]; ^[1]

Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA
Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA, Bredesen Center for Interdisciplinary Research and Graduate Education University of Tennessee Knoxville TN USA
Genome Science and Technology University of Tennessee Knoxville TN USA, Chemical Science Division Oak Ridge National Laboratory Oak Ridge TN USA
Noble Research Institute Ardmore OK USA
Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA, Chemical Science Division Oak Ridge National Laboratory Oak Ridge TN USA

Summary

Soil‐borne microbes can establish compatible relationships with host plants, providing a large variety of nutritive and protective compounds in exchange for photosynthesized sugars. However, the molecular mechanisms mediating the establishment of these beneficial relationships remain unclear. Our previous genetic mapping and whole‐genome resequencing studies identified a gene deletion event of a Populus trichocarpa lectin receptor‐like kinase gene PtLecRLK1 in Populus deltoides that was associated with poor‐root colonization by the ectomycorrhizal fungus Laccaria bicolor . By introducing PtLecRLK1 into a perennial grass known to be a non‐host of L. bicolor , switchgrass ( Panicum virgatum L.), we found that L. bicolor colonizes ZmUbipro‐PtLecRLK1 transgenic switchgrass roots, which illustrates that the introduction of PtLecRLK1 has the potential to convert a non‐host to a host of L. bicolor . Furthermore, transcriptomic and proteomic analyses on inoculated‐transgenic switchgrass roots revealed genes/proteins overrepresented in the compatible interaction and underrepresented in the pathogenic defence pathway, consistent with the view that pathogenic defence response is down‐regulated during compatible interaction. Metabolomic profiling revealed that root colonization in the transgenic switchgrass was associated with an increase in N‐containing metabolites and a decrease in organic acids, sugars, and aromatic hydroxycinnamate conjugates, which are often seen in the early steps of establishing compatible interactions. These studies illustrate that PtLecRLK1 is able to render a plant susceptible to colonization by the ectomycorrhizal fungus L. bicolor and shed light on engineering mycorrhizal symbiosis into a non‐host to enhance plant productivity and fitness on marginal lands.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: NONE; AC05-00OR22725

OSTI ID:: 1812816

Alternate ID(s):: OSTI ID: 1811404
OSTI ID: 1812817

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Journal Issue: 12 Vol. 19; ISSN 1467-7644

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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