Enzymes as parts in need of replacement - and how to extend their working life

Tivendale, Nathan D.; Hanson, Andrew D.; Henry, Christopher S.; Hegeman, Adrian D.; Millar, A. Harvey

doi:10.1016/j.tplants.2020.02.006

Enzymes as parts in need of replacement - and how to extend their working life

Journal Article · Wed Jul 01 00:00:00 EDT 2020 · Trends in Plant Science

DOI:https://doi.org/10.1016/j.tplants.2020.02.006· OSTI ID:1811305

Tivendale, Nathan D.; Hanson, Andrew D.; Henry, Christopher S.; Hegeman, Adrian D.; Millar, A. Harvey

Enzymes catalyze reactions in vivo at different rates and each enzyme molecule has a lifetime limit before it is degraded and replaced to enable catalysis to continue. Considering these rates together as a unitless ratio of catalytic cycles until replacement (CCR) provides a new quantitative tool to assess the replacement schedule of and energy investment into enzymes as they relate to function. Here, we outline the challenges of determining CCRs and new approaches to overcome them and then assess the CCRs of selected enzymes in bacteria and plants to reveal a range of seven orders of magnitude for this ratio. Modifying CCRs in plants holds promise to lower cellular costs, to tailor enzymes for particular environments, and to breed enzyme improvements for crop productivity.

Research Organization:: Argonne National Laboratory (ANL)

Sponsoring Organization:: Australian Research Council; National Science Foundation (NSF); USDOE Office of Science - Office of Biological and Environmental Research

DOE Contract Number:: AC02-06CH11357

OSTI ID:: 1811305

Journal Information:: Trends in Plant Science, Journal Name: Trends in Plant Science Journal Issue: 7 Vol. 25; ISSN 1360-1385

Country of Publication:: United States

Language:: English

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