High Throughput Mapping of Single Molecules’ Redox Potentials on Electrode

Lei, Chenghong; Hu, Dehong

doi:10.1021/acs.analchem.1c00984

High Throughput Mapping of Single Molecules’ Redox Potentials on Electrode

Journal Article · Thu Jun 17 00:00:00 EDT 2021 · Analytical Chemistry

DOI:https://doi.org/10.1021/acs.analchem.1c00984· OSTI ID:1808084

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Guilin University of Technology (China)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

By synchronizing electrochemical potential scanning with a single-molecule localization super-resolution fluorescence microscope, kinetic fluorescence changes of hundreds of single molecular redox events were tracked simultaneously with high throughput, and subsequent cross-correlation function analysis mapped single molecules’ redox potentials (times) out on the imaging area from site to site in unprecedented detail by extracting electrochemically induced fluorescence change from apparently random fluorescence on/off blinking. This work paves the way toward mapping redox states at single-molecule levels in high throughput in chemical and biological systems.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: Guilin University of Technology; National Natural Science Foundation of China (NSFC); Natural Science Foundation of Guangxi; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1808084

Report Number(s):: PNNL-SA--149316

Journal Information:: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 25 Vol. 93; ISSN 0003-2700

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
computational chemistry
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fluorescence
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High Throughput Mapping of Single Molecules’ Redox Potentials on Electrode

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