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Ecogenomics of Groundwater Phages Suggests Niche Differentiation Linked to Specific Environmental Tolerance

Journal Article · · mSystems
 [1];  [2];  [1];  [1];  [1];  [3];  [4];  [5];  [6];  [7];  [8];  [9];  [10];  [5];  [11];
  1. Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California, USA
  2. Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA
  3. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
  4. Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA, Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
  5. Earth and Environmental Sciences, Lawrence Berkeley National Laboratory, Berkeley, California, USA
  6. Center for Biofilm Engineering, Montana State University, Bozeman, Montana, USA
  7. Center for Biofilm Engineering, Montana State University, Bozeman, Montana, USA, Department of Microbiology &, Immunology, Montana State University, Bozeman, Montana, USA
  8. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA, Department of Plant and Microbial Biology, University of California, Berkeley, California, USA
  9. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA, Energy Biosciences Institute, Berkeley, California, USA, Department of Bioengineering, University of California, Berkeley, California, USA
  10. Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA, Broad Institute of MIT Cambridge, Cambridge, Massachusetts, USA, Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
  11. Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, California, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

To our knowledge, this is the first study to identify the bacteriophage distribution in a groundwater ecosystem shedding light on their prevalence and distribution across metal-contaminated and background sites. Our study is uniquely based on selective sequencing of solely the extrachromosomal elements of a microbiome followed by analysis for viral signatures, thus establishing a more focused approach for phage identifications.

Sponsoring Organization:
USDOE
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1804147
Alternate ID(s):
OSTI ID: 1807913
OSTI ID: 1826559
Journal Information:
mSystems, Journal Name: mSystems Journal Issue: 3 Vol. 6; ISSN 2379-5077
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English

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