Iron-Imprinted Single-Atomic Site Catalyst-Based Nanoprobe for Detection of Hydrogen Peroxide in Living Cells

Lyu, Zhaoyuan; Ding, Shichao; Wang, Maoyu; Pan, Xiaoqing; Feng, Zhenxing; Tian, Hangyu; Zhu, Chengzhou; Du, Dan; Lin, Yuehe

doi:10.1007/s40820-021-00661-z

Iron-Imprinted Single-Atomic Site Catalyst-Based Nanoprobe for Detection of Hydrogen Peroxide in Living Cells

Journal Article · Sat Jun 19 00:00:00 EDT 2021 · Nano-Micro Letters

DOI:https://doi.org/10.1007/s40820-021-00661-z· OSTI ID:1806326

Lyu, Zhaoyuan ^[1]; Ding, Shichao ^[1]; Wang, Maoyu ^[2]; Pan, Xiaoqing ^[3]; Feng, Zhenxing ^[2]; Tian, Hangyu ^[1]; Zhu, Chengzhou ^[1]; Du, Dan ^[1]; Lin, Yuehe ^[1]

Washington State Univ., Pullman, WA (United States)
Oregon State Univ., Corvallis, OR (United States)
Univ. of California, Irvine, CA (United States)

Fe-based single-atomic site catalysts (SASCs), with the natural metalloproteases-like active site structure, have attracted widespread attention in biocatalysis and biosensing. Precisely, controlling the isolated single-atom Fe-N-C active site structure is crucial to improve the SASCs’ performance. In this work, we use a facile ion-imprinting method (IIM) to synthesize isolated Fe-N-C single-atomic site catalysts (IIM-Fe-SASC). With this method, the ion-imprinting process can precisely control ion at the atomic level and form numerous well-defined single-atomic Fe-N-C sites. The IIM-Fe-SASC shows better peroxidase-like activities than that of non-imprinted references. Due to its excellent properties, IIM-Fe-SASC is an ideal nanoprobe used in the colorimetric biosensing of hydrogen peroxide (H₂O₂). Using IIM-Fe-SASC as the nanoprobe, in situ detection of H₂O₂ generated from MDA-MB-231 cells has been successfully demonstrated with satisfactory sensitivity and specificity. This work opens a novel and easy route in designing advanced SASC and provides a sensitive tool for intracellular H₂O₂ detection.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1806326

Journal Information:: Nano-Micro Letters, Journal Name: Nano-Micro Letters Journal Issue: 1 Vol. 13; ISSN 2311-6706

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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