Bacillus pumilus improved drought tolerance in Glycyrrhiza uralensis G5 seedlings through enhancing primary and secondary metabolisms

Zhang, Xinhui; Xie, Zhicai; Lang, Duoyong; Chu, Yuankui; Cui, Gaochang; Jia, Xiaoxia

doi:10.1111/ppl.13236

Bacillus pumilus improved drought tolerance in Glycyrrhiza uralensis G5 seedlings through enhancing primary and secondary metabolisms

Journal Article · Wed Oct 28 00:00:00 EDT 2020 · Physiologia Plantarum

DOI:https://doi.org/10.1111/ppl.13236· OSTI ID:1805095

^[1]; Xie, Zhicai ^[2]; Lang, Duoyong ^[3]; Chu, Yuankui ^[4]; Cui, Gaochang ^[2]; Jia, Xiaoxia ^[5]

College of Pharmacy Ningxia Medical University Yinchuan China, Ningxia Engineering and Technology Research Center of Hui Medicine Modernization, Ningxia Collaborative Innovation Center of Hui Medicine, Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education Ningxia Medical University Yinchuan China
College of Pharmacy Ningxia Medical University Yinchuan China
Laboratory Animal Center Ningxia Medical University Yinchuan China
Department of Laboratory Medicine General Hospital of Ningxia Medical University, Ningxia Medical University Yinchuan China
Potato Research Institute Gansu Academy of Agricultural Sciences Lanzhou China

Abstract

It has been reported that drought stress adversely affects the growth and yield of Glycyrrhiza uralensis, Chinese liquorice, in agricultural production. Bacillus pumilus , an important plant growth‐promoting bacterium, play a significant role in improving plant tolerance to abiotic stress. However, the role of Bacillus pumilus G5 in resisting drought stress is largely unknown. In the present study, we found that drought stress significantly inhibited the growth and reduced the biomass of G. uralensis seedlings by restraining C‐ and N‐metabolism, while this could be effectively reversed by B. pumilus G5 inoculation. Specifically, B. pumilus G5 significantly increased the content of primary metabolites such as soluble sugar, soluble protein, and free amino acids by regulating the C and N metabolic processes in G. uralensis seedlings. Moreover, B. pumilus G5 increased the content of glycyrrhizic acid, one of the important secondary metabolites, likely mediated through the increased content of primary metabolites and by recovering the expression of three key enzymes, HMGR, SQS, and β‐AS, in the biosynthesis of glycyrrhizic acid. Interestingly, the regulating effect of B. pumilus G5 inoculation on promoting the accumulation of glycyrrhizic acid and increasing the expression of synthesis‐related genes is spatially selective. In summary, our findings suggest that B. pumilus G5 could alleviate adverse effects induced by drought stress on the growth of G. uralensis seedlings by regulating C‐ and N‐metabolisms that further triggered the accumulation of secondary metabolites, and this finally improved the drought tolerance of cultivated G. uralensis seedlings.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

OSTI ID:: 1805095

Journal Information:: Physiologia Plantarum, Journal Name: Physiologia Plantarum Journal Issue: 3 Vol. 171; ISSN 0031-9317

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: Denmark

Language:: English

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