Reduction and bacterial adsorption of dissolved mercuric ion by indigenous bacteria at the Oak Ridge Reservation site

Li, Runwei; Qi, Lin; Ibeanusi, Victor; Badisa, Veera; Brooks, Scott C.; Chen, Gang

doi:10.1016/j.chemosphere.2021.130629

Reduction and bacterial adsorption of dissolved mercuric ion by indigenous bacteria at the Oak Ridge Reservation site

Journal Article · Fri Apr 23 00:00:00 EDT 2021 · Chemosphere

DOI:https://doi.org/10.1016/j.chemosphere.2021.130629· OSTI ID:1805007

Li, Runwei ^[1]; Qi, Lin ^[2]; Ibeanusi, Victor ^[1]; Badisa, Veera ^[1]; ^[3]; Chen, Gang ^[2]

Florida A & M University, Tallahassee, FL (United States)
Florida A&M Univ.-Florida State Univ. (FAMU-FSU), Tallahassee, FL (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Mercury exists in various forms in the environment and the indigenous bacteria mediated processes have the potential to be used for mercury remediation. In this study, two mixed cultures of indigenous bacteria at the Oak Ridge Reservation site (i.e., ORR soil culture and ORR sediment culture) were selected to study the microbial mediated mercuric reduction under an aerobic condition as well as mercury adsorption onto bacterial surfaces. PCR analysis was performed to provide insights into the microbial community. The mercuric volatilizing experiment demonstrated the mercuric reducing capacity for both ORR cultures, in which the Pseudomonas genus was the dominating Hg⁰ producer. The investigation of the impact of the sole carbon source revealed the energy-dependent characteristics of the mercuric reduction in this study. Namely, the mercuric reduction was nearly not impacted by the type of carbon source but positively related to the energy that a unit amount of substrate could provide. The study also indicated that the mercury adsorption competed with the reduction. According to the fitting of the Langmuir isotherm, the ORR soil culture was found to have a higher mercury adsorption capacity (i.e., 67.5 mg Hg/g dry biomass) than the ORR sediment culture (i.e., 53.1 mg Hg/g dry biomass). The negative correlation between the reduced mercury mass and adsorbed mercury mass was identified for both ORR cultures.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1805007

Alternate ID(s):: OSTI ID: 1797510

Journal Information:: Chemosphere, Journal Name: Chemosphere Journal Issue: 2021 Vol. 280; ISSN 0045-6535

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (37)

Biogeochemical controls on methylmercury in soils and sediments: Implications for site management: Geochemical Controls on Mercury Methylation Bigham, Gary N.; Murray, Karen J.; Masue-Slowey, Yoko Integrated Environmental Assessment and Management, Vol. 13, Issue 2 https://doi.org/10.1002/ieam.1822	journal	September 2016
Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction Zhang, Weiwei; Chen, Lingxin; Liu, Dongyan Applied Microbiology and Biotechnology, Vol. 93, Issue 3 https://doi.org/10.1007/s00253-011-3454-5	journal	July 2011
Tolerance to Various Toxicants by Marine Bacteria Highly Resistant to Mercury De, Jaysankar; Ramaiah, N.; Mesquita, A. Marine Biotechnology, Vol. 5, Issue 2 https://doi.org/10.1007/s10126-002-0061-6	journal	April 2003
Mercury in Marine and Oceanic Waters—a Review Gworek, Barbara; Bemowska-Kałabun, Olga; Kijeńska, Marta Water, Air, & Soil Pollution, Vol. 227, Issue 10 https://doi.org/10.1007/s11270-016-3060-3	journal	September 2016
Characterization and potential application in mercury bioremediation of highly mercury-resistant marine bacterium Bacillus thuringiensis PW-05 Dash, Hirak R.; Mangwani, Neelam; Das, Surajit Environmental Science and Pollution Research, Vol. 21, Issue 4 https://doi.org/10.1007/s11356-013-2206-8	journal	October 2013
A comparison of the thermodynamics of metal adsorption onto two common bacteria Daughney, Christopher J.; Fein, Jeremy B.; Yee, Nathan Chemical Geology, Vol. 144, Issue 3-4 https://doi.org/10.1016/S0009-2541(97)00124-1	journal	February 1998
Comparison of the acid-base behaviour and metal adsorption characteristics of a gram-negative bacterium with other strains Ngwenya, Bryne T.; Sutherland, Ian W.; Kennedy, Lynn Applied Geochemistry, Vol. 18, Issue 4 https://doi.org/10.1016/S0883-2927(02)00118-X	journal	April 2003
Bacterial-facilitated uranium transport in the presence of phytate at Savannah River Site Li, Runwei; Ibeanusi, Victor; Hoyle-Gardner, Jada Chemosphere, Vol. 223 https://doi.org/10.1016/j.chemosphere.2019.02.064	journal	May 2019
History of mercury use and environmental contamination at the Oak Ridge Y-12 Plant Brooks, Scott C.; Southworth, George R. Environmental Pollution, Vol. 159, Issue 1 https://doi.org/10.1016/j.envpol.2010.09.009	journal	January 2011
Elemental mercury: Its unique properties affect its behavior and fate in the environment Gonzalez-Raymat, Hansell; Liu, Guangliang; Liriano, Carolina Environmental Pollution, Vol. 229 https://doi.org/10.1016/j.envpol.2017.04.101	journal	October 2017
Bacterial community rather than metals shaping metal resistance genes in water, sediment and biofilm in lakes from arid northwestern China Song, Wenjuan; Qi, Ran; Zhao, Li Environmental Pollution, Vol. 254 https://doi.org/10.1016/j.envpol.2019.113041	journal	November 2019
Low dose mercury toxicity and human health Zahir, Farhana; Rizwi, Shamim J.; Haq, Soghra K. Environmental Toxicology and Pharmacology, Vol. 20, Issue 2 https://doi.org/10.1016/j.etap.2005.03.007	journal	September 2005
Studies on mercury bioremediation by alginate immobilized mercury tolerant Bacillus cereus cells Sinha, Arvind; Pant, Kamal Kishore; Khare, Sunil Kumar International Biodeterioration & Biodegradation, Vol. 71 https://doi.org/10.1016/j.ibiod.2011.12.014	journal	July 2012
Removal of mercury(II) from aqueous solution using moss (Drepanocladus revolvens) biomass: Equilibrium, thermodynamic and kinetic studies Sari, Ahmet; Tuzen, Mustafa Journal of Hazardous Materials, Vol. 171, Issue 1-3 https://doi.org/10.1016/j.jhazmat.2009.06.023	journal	November 2009
Guidelines for the use and interpretation of adsorption isotherm models: A review Al-Ghouti, Mohammad A.; Da'ana, Dana A. Journal of Hazardous Materials, Vol. 393 https://doi.org/10.1016/j.jhazmat.2020.122383	journal	July 2020
Abiotic methylation of mercury in the aquatic environment Celo, Valbona; Lean, David R. S.; Scott, Susannah L. Science of The Total Environment, Vol. 368, Issue 1 https://doi.org/10.1016/j.scitotenv.2005.09.043	journal	September 2006
Isolation of mercury resistant bacteria and influence of abiotic factors on bioavailability of mercury — A case study in Pulicat Lake North of Chennai, South East India Kannan, S. Kamala; Krishnamoorthy, R. Science of The Total Environment, Vol. 367, Issue 1 https://doi.org/10.1016/j.scitotenv.2005.12.003	journal	August 2006
Binding, distribution, and plant uptake of mercury in a soil from Oak Ridge, Tennessee, USA Han, Fengxiang X.; Su, Yi; Monts, David L. Science of The Total Environment, Vol. 368, Issue 2-3 https://doi.org/10.1016/j.scitotenv.2006.02.026	journal	September 2006
Non-indigenous microorganisms in the Antarctic: assessing the risks Cowan, Don A.; Chown, Steven L.; Convey, Peter Trends in Microbiology, Vol. 19, Issue 11 https://doi.org/10.1016/j.tim.2011.07.008	journal	November 2011
Determining the Reliability of Measuring Mercury Cycling Gene Abundance with Correlations with Mercury and Methylmercury Concentrations Christensen, Geoff A.; Gionfriddo, Caitlin M.; King, Andrew J. Environmental Science & Technology, Vol. 53, Issue 15 https://doi.org/10.1021/acs.est.8b06389	journal	July 2019
Mercury Physicochemical and Biogeochemical Transformation in the Atmosphere and at Atmospheric Interfaces: A Review and Future Directions Ariya, Parisa A.; Amyot, Marc; Dastoor, Ashu Chemical Reviews, Vol. 115, Issue 10 https://doi.org/10.1021/cr500667e	journal	February 2015
Mercury Stable Isotope Fractionation during Reduction of Hg(II) to Hg(0) by Mercury Resistant Microorganisms Kritee, K.; Blum, Joel D.; Johnson, Marcus W. Environmental Science & Technology, Vol. 41, Issue 6 https://doi.org/10.1021/es062019t	journal	March 2007
A Study on the Adsorption Mechanism of Mercury on Aspergillus versicolor Biomass Das, Sujoy K.; Das, Akhil R.; Guha, Arun K. Environmental Science & Technology, Vol. 41, Issue 24 https://doi.org/10.1021/es070814g	journal	December 2007
Mercury Reduction and Cell-Surface Adsorption by Geobacter sulfurreducens PCA Hu, Haiyan; Lin, Hui; Zheng, Wang Environmental Science & Technology, Vol. 47, Issue 19 https://doi.org/10.1021/es400527m	journal	September 2013
Mercury Methylation by Novel Microorganisms from New Environments Gilmour, Cynthia C.; Podar, Mircea; Bullock, Allyson L. Environmental Science & Technology, Vol. 47, Issue 20 https://doi.org/10.1021/es403075t	journal	September 2013
Biomagnification of Mercury in Aquatic Food Webs: A Worldwide Meta-Analysis Lavoie, Raphael A.; Jardine, Timothy D.; Chumchal, Matthew M. Environmental Science & Technology, Vol. 47, Issue 23 https://doi.org/10.1021/es403103t	journal	November 2013
Response of Mercury Contamination in Fish to Decreased Aqueous Concentrations and Loading of Inorganic Mercury in a Small Stream Southworth, George R.; Turner, Ralph R.; Peterson, Mark J. Environmental Monitoring and Assessment, Vol. 63, Issue 3, p. 481-494 https://doi.org/10.1023/A:1006237610383	journal	August 2000
DADA2: High-resolution sample inference from Illumina amplicon data Callahan, Benjamin J.; McMurdie, Paul J.; Rosen, Michael J. Nature Methods, Vol. 13, Issue 7 https://doi.org/10.1038/nmeth.3869	journal	May 2016
Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 Bolyen, Evan; Rideout, Jai Ram; Dillon, Matthew R. Nature Biotechnology, Vol. 37, Issue 8 https://doi.org/10.1038/s41587-019-0209-9	journal	July 2019
Roles of dissolved organic matter in the speciation of mercury and methylmercury in a contaminated ecosystem in Oak Ridge, Tennessee Dong, Wenming; Liang, Liyuan; Brooks, Scott Environmental Chemistry, Vol. 7, Issue 1 https://doi.org/10.1071/EN09091	journal	January 2010
Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample Caporaso, J. G.; Lauber, C. L.; Walters, W. A. Proceedings of the National Academy of Sciences, Vol. 108, Issue Supplement_1 https://doi.org/10.1073/pnas.1000080107	journal	June 2010
Cycling of mercury in the environment: Sources, fate, and human health implications: A review Beckers, Felix; Rinklebe, Jörg Critical Reviews in Environmental Science and Technology, Vol. 47, Issue 9 https://doi.org/10.1080/10643389.2017.1326277	journal	May 2017
Effects of Growth Medium, Inoculum Size, and Incubation Time on Culturability and Isolation of Soil Bacteria Davis, K. E. R.; Joseph, S. J.; Janssen, P. H. Applied and Environmental Microbiology, Vol. 71, Issue 2 https://doi.org/10.1128/AEM.71.2.826-834.2005	journal	February 2005
Methylmercury Decomposition in Sediments and Bacterial Cultures: Involvement of Methanogens and Sulfate Reducers in Oxidative Demethylation Oremland, Ronald S.; Culbertson, Charles W.; Winfrey, Michael R. Applied and Environmental Microbiology, Vol. 57, Issue 1 https://doi.org/10.1128/aem.57.1.130-137.1991	journal	January 1991
Global Biogeochemical Cycling of Mercury: A Review Selin, Noelle E. Annual Review of Environment and Resources, Vol. 34, Issue 1 https://doi.org/10.1146/annurev.environ.051308.084314	journal	November 2009
Cadmium–Bacteria Complexation and Subsequent Bacteria‐Facilitated Cadmium Transport in Saturated Porous Media Li, Runwei; Zhang, Zhiming; Li, Simeng Journal of Environmental Quality, Vol. 48, Issue 5 https://doi.org/10.2134/jeq2018.10.0369	journal	August 2019
Human Exposure and Health Effects of Inorganic and Elemental Mercury Park, Jung-Duck; Zheng, Wei Journal of Preventive Medicine & Public Health, Vol. 45, Issue 6 https://doi.org/10.3961/jpmph.2012.45.6.344	journal	November 2012

Similar Records

Adsorption and intracellular uptake of mercuric mercury and methylmercury by methanotrophs and methylating bacteria

Journal Article · Fri May 12 20:00:00 EDT 2023 · Environmental Pollution · OSTI ID:1984347

Bacterial removal of mercury from sewage

Journal Article · Wed Oct 31 23:00:00 EST 1984 · Biotechnol. Bioeng.; (United States) · OSTI ID:5758215

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Adsorption
Mercury
Microbial Community
Reduction

Reduction and bacterial adsorption of dissolved mercuric ion by indigenous bacteria at the Oak Ridge Reservation site

Citation Formats

References (37)

Similar Records

Related Subjects