Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment

Buongiorno, Joy; Sipes, Katie; Wasmund, Kenneth; Loy, Alexander; Lloyd, Karen G.; Girguis, ed., Peter R.

doi:10.1371/journal.pone.0234839

Title: Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment

Journal Article · Thu Aug 27 00:00:00 EDT 2020 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0234839· OSTI ID:1651175

; Sipes, Katie; Wasmund, Kenneth; Loy, Alexander; Lloyd, Karen G.; Girguis, ed., Peter R.

Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing change. This study combined 16S rRNA gene analysis, metagenome- assembled genomes (MAGs), and genome-resolved metatranscriptomics uncovered the in situ abundance and transcriptional activity of Woeseiales with burial in four shallow sediment sites of Kongsfjorden and Van Keulenfjorden of Svalbard (79˚N). We present five novel Woeseiales MAGs and show transcriptional evidence for metabolic plasticity during burial, including sulfur oxidation with reverse dissimilatory sulfite reductase (dsrAB) down to 4 cm depth and nitrite reduction down to 6 cm depth. A single stress protein, spore protein SP21 (hspA), had a tenfold higher mRNA abundance than any other transcript, and was a hundredfold higher on average than other transcripts. At three out of the four sites, SP21 transcript abundance increased with depth, while total mRNA abundance and richness decreased, indicating a shift in investment from metabolism and other cellular processes to build-up of spore protein SP21. The SP21 gene in MAGs was often flanked by genes involved in membrane-associated stress response. The ability of Woeseiales to shift from sulfur oxidation to nitrite reduction with burial into marine sediments with decreasing access to overlying oxic bottom waters, as well as enter into a dormant state dominated by SP21, may account for its ubiquity and high abundance in marine sediments worldwide, including those of the rapidly shifting Arctic.

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Research Organization:: Univ. of Tennessee, Knoxville, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Simons Foundation; Austrian Science Fund (FWF)

Grant/Contract Number:: SC0020369; 404586; P29426-B29; P25111-B22

OSTI ID:: 1651175

Alternate ID(s):: OSTI ID: 1803970; OSTI ID: 1991906

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Vol. 15 Journal Issue: 8; ISSN 1932-6203

Publisher:: Public Library of Science (PLoS)Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment

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