¡Viva la mitochondria!: harnessing yeast mitochondria for chemical production

Duran, Lisset; López, José Montaño; Avalos, José L.

doi:10.1093/femsyr/foaa037

¡Viva la mitochondria!: harnessing yeast mitochondria for chemical production

Journal Article · Sat Jun 27 04:00:00 EDT 2020 · FEMS Yeast Research

DOI:https://doi.org/10.1093/femsyr/foaa037· OSTI ID:1803728

Duran, Lisset ^[1]; López, José Montaño ^[2]; ^[3]

Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA; OSTI
Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA; Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA; Andlinger Center for Energy and the Environment, Princeton University, Princeton, NJ 08544, USA; Princeton Environmental Institute, Princeton University, Princeton, NJ 08544, USA

ABSTRACT

The mitochondria, often referred to as the powerhouse of the cell, offer a unique physicochemical environment enriched with a distinct set of enzymes, metabolites and cofactors ready to be exploited for metabolic engineering. In this review, we discuss how the mitochondrion has been engineered in the traditional sense of metabolic engineering or completely bypassed for chemical production. We then describe the more recent approach of harnessing the mitochondria to compartmentalize engineered metabolic pathways, including for the production of alcohols, terpenoids, sterols, organic acids and other valuable products. We explain the different mechanisms by which mitochondrial compartmentalization benefits engineered metabolic pathways to boost chemical production. Finally, we discuss the key challenges that need to be overcome to expand the applicability of mitochondrial engineering and reach the full potential of this emerging field.

Research Organization:: Princeton Univ., NJ (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: SC0019363

OSTI ID:: 1803728

Journal Information:: FEMS Yeast Research, Journal Name: FEMS Yeast Research Journal Issue: 6 Vol. 20; ISSN 1567-1356

Country of Publication:: United States

Language:: English

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Mitochondrial engineering of the TCA cycle for fumarate production Chen, Xiulai; Dong, Xiaoxiang; Wang, Yuancai Metabolic Engineering, Vol. 31 https://doi.org/10.1016/j.ymben.2015.02.002	journal	September 2015
Identification of the yeast ARG-11 gene as a mitochondrial ornithine carrier involved in arginine biosynthesis Palmieri, Luigi; De Marco, Valeria; Iacobazzi, Vito FEBS Letters, Vol. 410, Issue 2-3 https://doi.org/10.1016/S0014-5793(97)00630-3	journal	June 1997
Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol degradation pathway and expression of the heterologous phosphoketolase pathway de Jong, Bouke Wim; Shi, Shuobo; Siewers, Verena Microbial Cell Factories, Vol. 13, Issue 1 https://doi.org/10.1186/1475-2859-13-39	journal	January 2014
Improving isobutanol production with the yeast Saccharomyces cerevisiae by successively blocking competing metabolic pathways as well as ethanol and glycerol formation Wess, Johannes; Brinek, Martin; Boles, Eckhard Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1486-8	journal	July 2019
Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism Chen, Yun; Daviet, Laurent; Schalk, Michel Metabolic Engineering, Vol. 15 https://doi.org/10.1016/j.ymben.2012.11.002	journal	January 2013
In vivo measurement of cytosolic and mitochondrial pH using a pH-sensitive GFP derivative in Saccharomyces cerevisiae reveals a relation between intracellular pH and growth Orij, R.; Postmus, J.; Ter Beek, A. Microbiology, Vol. 155, Issue 1 https://doi.org/10.1099/mic.0.022038-0	journal	January 2009
Metabolically engineered Saccharomyces cerevisiae for branched-chain ester productions Yuan, Jifeng; Mishra, Pranjul; Ching, Chi Bun Journal of Biotechnology, Vol. 239 https://doi.org/10.1016/j.jbiotec.2016.10.013	journal	December 2016
Cytosolic re-localization and optimization of valine synthesis and catabolism enables inseased isobutanol production with the yeast Saccharomyces cerevisiae Brat, Dawid; Weber, Christian; Lorenzen, Wolfram Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-65	journal	January 2012
Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine Qin, Jiufu; Zhou, Yongjin J.; Krivoruchko, Anastasia Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms9224	journal	September 2015
Autoregulation may control the expression of yeast pyruvate decarboxylase structural genes PDC1 and PDC5 Hohmann, Stefan; Cederberg, Hakan European Journal of Biochemistry, Vol. 188, Issue 3 https://doi.org/10.1111/j.1432-1033.1990.tb15442.x	journal	March 1990
The Yeast Mitochondrial Carrier Leu5p and Its Human Homologue Graves' Disease Protein Are Required for Accumulation of Coenzyme A in the Matrix Prohl, Corinna; Pelzer, Winfried; Diekert, Kerstin Molecular and Cellular Biology, Vol. 21, Issue 4 https://doi.org/10.1128/MCB.21.4.1089-1097.2001	journal	February 2001
The signal that sorts yeast cytochrome b2 to the mitochondrial intermembrane space contains three distinct functional regions. Beasley, E. M.; Müller, S.; Schatz, G. The EMBO Journal, Vol. 12, Issue 6 https://doi.org/10.1002/j.1460-2075.1993.tb05884.x	journal	June 1993

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