New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells

Souza, Ana Camila Oliveira; Favali, Cecília; Soares, Naiara Caroline; Tavares, Natalia Machado; Jerônimo, Márcio Sousa; Veloso Junior, Paulo Henrique; Marina, Clara Luna; Santos, Claire; Brodskyn, Cláudia; Bocca, Anamelia Lorenzetti

doi:10.3389/fmicb.2019.02445

Title: New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells

Journal Article · Wed Oct 30 00:00:00 EDT 2019 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2019.02445· OSTI ID:1802756

Souza, Ana Camila Oliveira ^[1]; Favali, Cecília ^[1]; Soares, Naiara Caroline ^[1]; Tavares, Natalia Machado ^[2]; Jerônimo, Márcio Sousa ^[1]; Veloso Junior, Paulo Henrique ^[1]; Marina, Clara Luna ^[1]; Santos, Claire ^[2]; Brodskyn, Cláudia ^[2]; Bocca, Anamelia Lorenzetti ^[1]

Universidade de Brasília, Brasília (Brazil). Departamento de Biologia Celular
Fundação Oswaldo Cruz, Salvador (Brazil). Centro de Pesquisas Gonçalo Moniz

The cell wall has a critical role in the host immune response to fungal pathogens. In this study, we investigated the influence of two cell wall fractions of the dimorphic fungi Paracoccidioides brasiliensis (Pb) in the in vitro generation of monocyte-derived dendritic cells (MoDCs). Monocytes were purified from the peripheral blood of healthy donors and cultivated for 7 days in medium supplemented with IL-4 and GM-CSF in the presence of Pb cell wall fractions: the alkali-insoluble F1, constituted by b-1,3-glucans, chitin and proteins, and the alkali-soluble F2, mainly constituted by a-glucan. MoDCs phenotypes were evaluated regarding cell surface expression of CD1a, DCSIGN, HLA-DR, CD80, and CD83 and production of cytokines. The a-glucan-rich cell wall fraction downregulated the differentiation of CD1aC MoDCs, a dendritic cell subset that stimulate Th1 responses. The presence of both cell fractions inhibited DC-SIGN and HLA-DR expression, while the expression of maturation markers was differentially induced in CD1a- MoDCs. Differentiation upon F1 and F2 stimulation induced mixed profile of inflammatory cytokines. Altogether, these data demonstrate that Pb cell wall fractions differentially induce a dysregulation in DCs differentiation. Moreover, our results suggest that cell wall a-glucan promote the differentiation of CD1a- DCs, potentially favoring Th2 polarization and contributing to pathogen persistence.

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Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0015662

OSTI ID:: 1802756

Alternate ID(s):: OSTI ID: 1964128

Journal Information:: Frontiers in Microbiology, Vol. 10; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Title: New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells

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