Evolution-inspired design of multicolored photoswitches from a single cyanobacteriochrome scaffold

Fushimi, Keiji; Hasegawa, Masumi; Ito, Takeru; Rockwell, Nathan C.; Enomoto, Gen; -Win, Ni-Ni; Lagarias, J. Clark; Ikeuchi, Masahiko; Narikawa, Rei

doi:10.1073/pnas.2004273117

Evolution-inspired design of multicolored photoswitches from a single cyanobacteriochrome scaffold

Journal Article · Mon Jun 22 00:00:00 EDT 2020 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.2004273117· OSTI ID:1634234

Fushimi, Keiji ^[1]; Hasegawa, Masumi ^[2]; Ito, Takeru ^[2]; Rockwell, Nathan C. ^[3]; Enomoto, Gen ^[4]; -Win, Ni-Ni ^[5]; ^[3]; Ikeuchi, Masahiko ^[4]; ^[6]

Department of Biological Science, Faculty of Science, Shizuoka University, Suruga, Shizuoka 422-8529, Japan,, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan,
Department of Biological Science, Faculty of Science, Shizuoka University, Suruga, Shizuoka 422-8529, Japan,
Department of Molecular and Cellular Biology, University of California, Davis, CA 95616,
Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan,, Graduate School of Arts and Sciences, University of Tokyo, Meguro, Tokyo 153-8902, Japan,
Graduate School of Arts and Sciences, University of Tokyo, Meguro, Tokyo 153-8902, Japan,
Department of Biological Science, Faculty of Science, Shizuoka University, Suruga, Shizuoka 422-8529, Japan,, Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan,, Research Institute of Green Science and Technology, Shizuoka University, Suruga, Shizuoka 422-8529, Japan

Significance

Cyanobacteriochromes (CBCRs) are small cyanobacterial photoreceptors which are highly diversified and categorized into many lineages based on their primary sequences. In this study, we identified an atypical CBCR exhibiting an orange/green reversible photocycle. Step-by-step site-directed mutagenesis was performed on this native CBCR, and seven new photoconvertible variants were created. During this process, we identified residues crucial for each color tuning event. These seven molecules covering the shorter-wavelength blue-to-orange region would contribute to the future development of multicolored optogenetic tools and are complementary to recently developed molecules sensing longer wavelengths of light.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0002395; SC0002395

OSTI ID:: 1634234

Alternate ID(s):: OSTI ID: 1801575

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 27 Vol. 117; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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