Identification and analysis of stem‐specific promoters from sugarcane and energy cane for oil accumulation in their stems

Wang, Jiang; Li, Yaxin; Wai, Ching Man; Beuchat, Gabriel; Chen, Li‐Qing

doi:10.1111/gcbb.12872

Title: Identification and analysis of stem‐specific promoters from sugarcane and energy cane for oil accumulation in their stems

Journal Article · Sun Jun 27 00:00:00 EDT 2021 · Global Change Biology. Bioenergy

DOI:https://doi.org/10.1111/gcbb.12872· OSTI ID:1798910

^[1]; Li, Yaxin ^[2]; Wai, Ching Man ^[3]; Beuchat, Gabriel ^[2];

^[1]

Carl R. Woese Institute for Genomic Biology University of Illinois at Urbana‐Champaign Urbana IL USA, Department of Plant Biology University of Illinois at Urbana‐Champaign Urbana IL USA
Department of Plant Biology University of Illinois at Urbana‐Champaign Urbana IL USA
Department of Horticulture Michigan State University East Lansing MI USA

Abstract Considerable recent progress has been achieved in bioengineering oil accumulation in the vegetative tissues of plants, opening an opportunity for large scale production of biodiesel, jet fuel, lubricants, and high‐value lipid bioproducts. For the highly productive C4 crops, such as sugarcane, energy cane, Miscanthus, and fiber sorghums, the bulk of the biomass is the stem. However, little success has been made in accumulating oil in the stem. Since engineering a trait with a constitutive promoter often results in pleiotropic effects that counter trait improvement, identification of stem parenchyma‐specific promoters is a prerequisite for efficient use of the ample photoassimilates stored in mature stem parenchyma cells. In this study, we first identified two TST genes encoding homologues of tonoplast sugar transporters that were strongly and almost exclusively expressed in the stems of canes via a combination of RNA‐seq atlas analysis, in silico analysis of a sugarcane genome, phylogenetic analysis, and quantitative PCR analysis. They were further confirmed in the pith parenchyma cells of the mature stem by RNA in situ hybridization. When fused with the β‐Glucuronidase (GUS) reporter gene, the promoters of two alleles, TST2b‐1A and TST2b‐1C , from one TST gene demonstrated that they could drive the GUS expression exclusively in the stem in Arabidopsis.

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Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018254

OSTI ID:: 1798910

Alternate ID(s):: OSTI ID: 1798911; OSTI ID: 1819189

Journal Information:: Global Change Biology. Bioenergy, Journal Name: Global Change Biology. Bioenergy Vol. 13 Journal Issue: 9; ISSN 1757-1693

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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09 BIOMASS FUELS
biofuel
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Title: Identification and analysis of stem‐specific promoters from sugarcane and energy cane for oil accumulation in their stems

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References (48)

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