Repurposing a microfluidic formulation device for automated DNA construction

Goyal, Garima; Elsbree, Nick; Fero, Michael; Hillson, Nathan J.; Linshiz, Gregory

doi:10.1371/journal.pone.0242157

Repurposing a microfluidic formulation device for automated DNA construction

Journal Article · Tue Nov 10 23:00:00 EST 2020 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0242157· OSTI ID:1798206

^[1]; Elsbree, Nick ^[2]; ^[2]; Hillson, Nathan J. ^[1]; Linshiz, Gregory ^[1]

Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems & Engineering Division
TeselaGen Biotechnology, San Francisco, CA (United States)

Microfluidic applications have expanded greatly over the past decade. For the most part, however, each microfluidics platform is developed with a specific task in mind, rather than as a general-purpose device with a wide-range of functionality. Here, we show how a microfluidic system, originally developed to investigate protein phase behavior, can be modified and repurposed for another application, namely DNA construction. We added new programable controllers to direct the flow of reagents across the chip. We designed the assembly of a combinatorial Golden Gate DNA library using TeselaGen DESIGN software and used the repurposed microfluidics platform to assemble the designed library from off-chip prepared DNA assembly pieces. Further experiments verified the sequences and function of the on-chip assembled DNA constructs.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1798206

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 11 Vol. 15; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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