Degradation of aromatic compounds and lignin by marine protist Thraustochytrium striatum

Li, Xiang; Li, Mi; Pu, Yunqiao Joseph; Ragauskas, Arthur J.; Tharayil, Nishanth; Huang, Jiale; Zheng, Yi

doi:10.1016/j.procbio.2021.05.006

Degradation of aromatic compounds and lignin by marine protist Thraustochytrium striatum

Journal Article · Wed May 12 00:00:00 EDT 2021 · Process Biochemistry

DOI:https://doi.org/10.1016/j.procbio.2021.05.006· OSTI ID:1787927

Li, Xiang ^[1]; ^[2]; ^[3]; ^[2]; Tharayil, Nishanth ^[4]; Huang, Jiale ^[5]; Zheng, Yi ^[6]

Kansas State Univ., Manhattan, KS (United States); Chinese Academy of Sciences (CAS), Shanghai (China)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Clemson Univ., SC (United States)
Xiamen Univ. (China)
Kansas State Univ., Manhattan, KS (United States)

This paper aimed at studying a novel high biomass strain (HBS) of marine protist, Thraustochytrium striatum, for lignin valorization to lipids and carotenoids. In the screening of 15 aromatic compounds, T. striatum HBS can utilize most compounds while achieving the best growth on 3,4-dihydroxybenzoic acid (3,4-DHBA) and 4-hydroxybenzoic acid (4-HBA). The maximum cell mass concentration reached 2-3 g/L under 5 g/L of 3,4-DHBA or 2 g/L of 4-HBA, which was comparable to that under equivalent concentration of glucose. The long-chain fatty acids content was 13 % and 7 % with 3,4-DHBA and 4-HBA, respectively. The adoption of fed-batch fermentation mode improved the degradation of carbon source and prevented the inhibitory effect caused by overloading of aromatic compounds. Further, both final cellular fatty acids and carotenoids contents were doubled in the fermentation of 3,4-DHBA. T. striatum HBS exhibited limited lignin depolymerization (~24 %) ability on technical lignins.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1787927

Journal Information:: Process Biochemistry, Journal Name: Process Biochemistry Vol. 107; ISSN 1359-5113

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (19)

Limited life cycle and cost assessment for the bioconversion of lignin‐derived aromatics into adipic acid Duuren, Jozef B. J. H.; Wild, Paul J.; Starck, Sören Biotechnology and Bioengineering, Vol. 117, Issue 5 https://doi.org/10.1002/bit.27299	journal	February 2020
Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil Margesin, Rosa; Volgger, Georg; Wagner, Andreas O. Applied Microbiology and Biotechnology, Vol. 105, Issue 7 https://doi.org/10.1007/s00253-021-11215-z	journal	March 2021
Production of Lipids and Proteome Variation in a Chilean Thraustochytrium striatum Strain Cultured under Different Growth Conditions Shene, Carolina; Garcés, Marcelo; Vergara, Daniela Marine Biotechnology, Vol. 21, Issue 1 https://doi.org/10.1007/s10126-018-9863-z	journal	November 2018
Investigation on the effects of cultivation conditions, fed-batch operation, and enzymatic hydrolysate of corn stover on the astaxanthin production by Thraustochytrium striatum Xiao, Rui; Li, Xiang; Leonard, Elizabeth Algal Research, Vol. 39 https://doi.org/10.1016/j.algal.2019.101475	journal	May 2019
Cultivation of microalgae for oil production with a cultivation strategy of urea limitation Hsieh, Chih-Hung; Wu, Wen-Teng Bioresource Technology, Vol. 100, Issue 17 https://doi.org/10.1016/j.biortech.2009.03.019	journal	September 2009
Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation Cheirsilp, Benjamas; Torpee, Salwa Bioresource Technology, Vol. 110 https://doi.org/10.1016/j.biortech.2012.01.125	journal	April 2012
Lignin-enzyme interaction: Mechanism, mitigation approach, modeling, and research prospects Li, Xiang; Zheng, Yi Biotechnology Advances, Vol. 35, Issue 4 https://doi.org/10.1016/j.biotechadv.2017.03.010	journal	July 2017
Permeabilization of Haematococcus pluvialis and solid-liquid extraction of astaxanthin by CO2-based alkyl carbamate ionic liquids Khoo, Kuan Shiong; Ooi, Chien Wei; Chew, Kit Wayne Chemical Engineering Journal, Vol. 411 https://doi.org/10.1016/j.cej.2021.128510	journal	May 2021
Directed bioconversion of Kraft lignin to polyhydroxyalkanoate by Cupriavidus basilensis B-8 without any pretreatment Shi, Yan; Yan, Xu; Li, Qiang Process Biochemistry, Vol. 52 https://doi.org/10.1016/j.procbio.2016.10.004	journal	January 2017
Inhibitory effects of lignin on enzymatic hydrolysis: The role of lignin chemistry and molecular weight Li, Xiang; Li, Mi; Pu, Yunqiao Renewable Energy, Vol. 123 https://doi.org/10.1016/j.renene.2018.02.079	journal	August 2018
Screening of Marine Bacteria To Synthesize Polyhydroxyalkanoate from Lignin: Contribution of Lignin Derivatives to Biosynthesis by Oceanimonas doudoroffii Numata, Keiji; Morisaki, Kumiko ACS Sustainable Chemistry & Engineering, Vol. 3, Issue 4 https://doi.org/10.1021/acssuschemeng.5b00031	journal	February 2015
Understanding the Limitations in the Biosynthesis of Polyhydroxyalkanoate (PHA) from Lignin Derivatives Tomizawa, Satoshi; Chuah, Jo-Ann; Matsumoto, Keiji ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 5 https://doi.org/10.1021/sc500066f	journal	March 2014
Bioconversion of oxygen-pretreated Kraft lignin to microbial lipid with oleaginous Rhodococcus opacus DSM 1069 Wei, Zhen; Zeng, Guangming; Huang, Fang Green Chemistry, Vol. 17, Issue 5 https://doi.org/10.1039/C5GC00422E	journal	January 2015
Synergistic enzymatic and microbial lignin conversion Zhao, Cheng; Xie, Shangxian; Pu, Yunqiao Green Chemistry, Vol. 18, Issue 5 https://doi.org/10.1039/C5GC01955A	journal	January 2016
Lignin to lipid bioconversion by oleaginous Rhodococci Kosa, Matyas; Ragauskas, Arthur J. Green Chemistry, Vol. 15, Issue 8 https://doi.org/10.1039/c3gc40434j	journal	January 2013
Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin Salvachúa, Davinia; Rydzak, Thomas; Auwae, Raquel Microbial Biotechnology, Vol. 13, Issue 1 https://doi.org/10.1111/1751-7915.13481	journal	January 2020
Isolation of bacterial strains able to metabolize lignin from screening of environmental samples Taylor, C. R.; Hardiman, E. M.; Ahmad, M. Journal of Applied Microbiology, Vol. 113, Issue 3 https://doi.org/10.1111/j.1365-2672.2012.05352.x	journal	June 2012
Bacillus amyloliquefaciens CotA degradation of the lignin model compound guaiacylglycerol-β-guaiacyl ether Yang, J.; Gao, M. Y.; Li, M. Letters in Applied Microbiology, Vol. 67, Issue 5 https://doi.org/10.1111/lam.13060	journal	September 2018
Metabolic engineering of Rhodococcus jostii RHA1 for production of pyridine-dicarboxylic acids from lignin Spence, Edward M.; Calvo-Bado, Leonides; Mines, Paul Microbial Cell Factories, Vol. 20, Issue 1 https://doi.org/10.1186/s12934-020-01504-z	journal	January 2021

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59 BASIC BIOLOGICAL SCIENCES
Aromatic compound
Carotenoid
Fatty acid
Fermentation
Lignin bioconversion
Thraustochytrium striatum

Degradation of aromatic compounds and lignin by marine protist Thraustochytrium striatum

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References (19)

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