Testing at scale during the COVID-19 pandemic

Mercer, Tim R.; Salit, Marc

doi:10.1038/s41576-021-00360-w

Testing at scale during the COVID-19 pandemic

Journal Article · Tue May 04 00:00:00 EDT 2021 · Nature Reviews. Genetics

DOI:https://doi.org/10.1038/s41576-021-00360-w· OSTI ID:1787603

Mercer, Tim R. ^[1]; Salit, Marc ^[2]

Univ. of Queensland, Brisbane, QLD (Australia); Garvan Institute of Medical Research, Sydney, NSW (Australia); Univ. of New South Wales, Sydney, NSW (Australia)
Stanford Univ., CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States)

Assembly and publication of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome in January 2020 enabled the immediate development of tests to detect the new virus. This began the largest global testing programme in history, in which hundreds of millions of individuals have been tested to date. The unprecedented scale of testing has driven innovation in the strategies, technologies and concepts that govern testing in public health. In this Review, we describe the changing role of testing during the COVID-19 pandemic, including the use of genomic surveillance to track SARS-CoV-2 transmission around the world, the use of contact tracing to contain disease outbreaks and testing for the presence of the virus circulating in the environment. Despite these efforts, widespread community transmission has become entrenched in many countries and has required the testing of populations to identify and isolate infected individuals, many of whom are asymptomatic. The diagnostic and epidemiological principles that underpin such population-scale testing are also considered, as are the high-throughput and point-of-care technologies that make testing feasible on a massive scale.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1787603

Journal Information:: Nature Reviews. Genetics, Journal Name: Nature Reviews. Genetics Journal Issue: 7 Vol. 22; ISSN 1471-0056

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

References (140)

Response to COVID-19 in Taiwan: Big Data Analytics, New Technology, and Proactive Testing Wang, C. Jason; Ng, Chun Y.; Brook, Robert H. JAMA, Vol. 323, Issue 14 https://doi.org/10.1001/jama.2020.3151	journal	April 2020
The Promise and Peril of Antibody Testing for COVID-19 Abbasi, Jennifer JAMA, Vol. 323, Issue 19 https://doi.org/10.1001/jama.2020.6170	journal	May 2020
Interpreting Diagnostic Tests for SARS-CoV-2 Sethuraman, Nandini; Jeremiah, Sundararaj Stanleyraj; Ryo, Akihide JAMA, Vol. 323, Issue 22 https://doi.org/10.1001/jama.2020.8259	journal	June 2020
Assessment of SARS-CoV-2 Screening Strategies to Permit the Safe Reopening of College Campuses in the United States Paltiel, A. David; Zheng, Amy; Walensky, Rochelle P. JAMA Network Open, Vol. 3, Issue 7 https://doi.org/10.1001/jamanetworkopen.2020.16818	journal	July 2020
Data, disease and diplomacy: GISAID's innovative contribution to global health: Data, Disease and Diplomacy Elbe, Stefan; Buckland-Merrett, Gemma Global Challenges, Vol. 1, Issue 1 https://doi.org/10.1002/gch2.1018	journal	January 2017
Assessing the dilution effect of specimen pooling on the sensitivity of SARS‐CoV‐2 PCR tests Bateman, Allen C.; Mueller, Shanna; Guenther, Kyley Journal of Medical Virology, Vol. 93, Issue 3 https://doi.org/10.1002/jmv.26519	journal	September 2020
Developing antibody tests for SARS-CoV-2 Petherick, Anna The Lancet, Vol. 395, Issue 10230 https://doi.org/10.1016/S0140-6736(20)30788-1	journal	April 2020
Prevalence of SARS-CoV-2 in Spain (ENE-COVID): a nationwide, population-based seroepidemiological study Pollán, Marina; Pérez-Gómez, Beatriz; Pastor-Barriuso, Roberto The Lancet, Vol. 396, Issue 10250 https://doi.org/10.1016/S0140-6736(20)31483-5	journal	August 2020
Slovakia to test all adults for SARS-CoV-2 Holt, Ed The Lancet, Vol. 396, Issue 10260 https://doi.org/10.1016/S0140-6736(20)32261-3	journal	October 2020
Buyer beware: inflated claims of sensitivity for rapid COVID-19 tests Fitzpatrick, Meagan C.; Pandey, Abhishek; Wells, Chad R. The Lancet, Vol. 397, Issue 10268 https://doi.org/10.1016/S0140-6736(20)32635-0	journal	January 2021
The impact of the COVID-19 pandemic on cancer deaths due to delays in diagnosis in England, UK: a national, population-based, modelling study Maringe, Camille; Spicer, James; Morris, Melanie The Lancet Oncology, Vol. 21, Issue 8 https://doi.org/10.1016/S1470-2045(20)30388-0	journal	August 2020
Viral load of SARS-CoV-2 in clinical samples Pan, Yang; Zhang, Daitao; Yang, Peng The Lancet Infectious Diseases, Vol. 20, Issue 4 https://doi.org/10.1016/S1473-3099(20)30113-4	journal	April 2020
Early dynamics of transmission and control of COVID-19: a mathematical modelling study Kucharski, Adam J.; Russell, Timothy W.; Diamond, Charlie The Lancet Infectious Diseases, Vol. 20, Issue 5 https://doi.org/10.1016/S1473-3099(20)30144-4	journal	May 2020
Effectiveness of isolation, testing, contact tracing, and physical distancing on reducing transmission of SARS-CoV-2 in different settings: a mathematical modelling study Kucharski, Adam J.; Klepac, Petra; Conlan, Andrew J. K. The Lancet Infectious Diseases, Vol. 20, Issue 10 https://doi.org/10.1016/S1473-3099(20)30457-6	journal	October 2020
Rapid implementation of SARS-CoV-2 sequencing to investigate cases of health-care associated COVID-19: a prospective genomic surveillance study Meredith, Luke W.; Hamilton, William L.; Warne, Ben The Lancet Infectious Diseases, Vol. 20, Issue 11 https://doi.org/10.1016/S1473-3099(20)30562-4	journal	November 2020
The temporal association of introducing and lifting non-pharmaceutical interventions with the time-varying reproduction number (R) of SARS-CoV-2: a modelling study across 131 countries Li, You; Campbell, Harry; Kulkarni, Durga The Lancet Infectious Diseases, Vol. 21, Issue 2 https://doi.org/10.1016/S1473-3099(20)30785-4	journal	February 2021
Scaling up COVID-19 rapid antigen tests: promises and challenges Peeling, Rosanna W.; Olliaro, Piero L.; Boeras, Debrah I. The Lancet Infectious Diseases https://doi.org/10.1016/S1473-3099(21)00048-7	journal	February 2021
Feasibility of controlling COVID-19 outbreaks by isolation of cases and contacts Hellewell, Joel; Abbott, Sam; Gimma, Amy The Lancet Global Health, Vol. 8, Issue 4 https://doi.org/10.1016/S2214-109X(20)30074-7	journal	April 2020
Prolonged presence of SARS-CoV-2 viral RNA in faecal samples Wu, Yongjian; Guo, Cheng; Tang, Lantian The Lancet Gastroenterology & Hepatology, Vol. 5, Issue 5 https://doi.org/10.1016/S2468-1253(20)30083-2	journal	May 2020
SARS-CoV-2 in wastewater: potential health risk, but also data source Lodder, Willemijn; de Roda Husman, Ana Maria The Lancet Gastroenterology & Hepatology, Vol. 5, Issue 6 https://doi.org/10.1016/S2468-1253(20)30087-X	journal	June 2020
Impact of delays on effectiveness of contact tracing strategies for COVID-19: a modelling study Kretzschmar, Mirjam E.; Rozhnova, Ganna; Bootsma, Martin C. J. The Lancet Public Health, Vol. 5, Issue 8 https://doi.org/10.1016/S2468-2667(20)30157-2	journal	August 2020
The challenges of informative wastewater sampling for SARS-CoV-2 must be met: lessons from polio eradication O'Reilly, Kathleen M.; Allen, David J.; Fine, Paul The Lancet Microbe, Vol. 1, Issue 5 https://doi.org/10.1016/S2666-5247(20)30100-2	journal	September 2020
A locally sustainable approach to COVID-19 testing in Africa Songok, Elijah The Lancet Microbe, Vol. 1, Issue 5 https://doi.org/10.1016/S2666-5247(20)30118-X	journal	September 2020
Humoral response and PCR positivity in patients with COVID-19 in the New York City region, USA: an observational study Wajnberg, Ania; Mansour, Mayce; Leven, Emily The Lancet Microbe, Vol. 1, Issue 7 https://doi.org/10.1016/S2666-5247(20)30120-8	journal	November 2020
SARS-CoV-2, SARS-CoV, and MERS-CoV viral load dynamics, duration of viral shedding, and infectiousness: a systematic review and meta-analysis Cevik, Muge; Tate, Matthew; Lloyd, Ollie The Lancet Microbe, Vol. 2, Issue 1 https://doi.org/10.1016/S2666-5247(20)30172-5	journal	January 2021
Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020 Liu, Rui; Han, Huan; Liu, Fang Clinica Chimica Acta, Vol. 505 https://doi.org/10.1016/j.cca.2020.03.009	journal	June 2020
Coast-to-Coast Spread of SARS-CoV-2 during the Early Epidemic in the United States Fauver, Joseph R.; Petrone, Mary E.; Hodcroft, Emma B. Cell, Vol. 181, Issue 5 https://doi.org/10.1016/j.cell.2020.04.021	journal	May 2020
Genomic Epidemiology of SARS-CoV-2 in Guangdong Province, China Lu, Jing; du Plessis, Louis; Liu, Zhe Cell, Vol. 181, Issue 5 https://doi.org/10.1016/j.cell.2020.04.023	journal	May 2020
Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus Korber, Bette; Fischer, Will M.; Gnanakaran, Sandrasegaram Cell, Vol. 182, Issue 4 https://doi.org/10.1016/j.cell.2020.06.043	journal	August 2020
Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19 Rodda, Lauren B.; Netland, Jason; Shehata, Laila Cell, Vol. 184, Issue 1 https://doi.org/10.1016/j.cell.2020.11.029	journal	January 2021
Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity Thomson, Emma C.; Rosen, Laura E.; Shepherd, James G. Cell, Vol. 184, Issue 5 https://doi.org/10.1016/j.cell.2021.01.037	journal	March 2021
Low performance of rapid antigen detection test as frontline testing for COVID-19 diagnosis Scohy, Anaïs; Anantharajah, Ahalieyah; Bodéus, Monique Journal of Clinical Virology, Vol. 129 https://doi.org/10.1016/j.jcv.2020.104455	journal	August 2020
Evaluation of rapid antigen test for detection of SARS-CoV-2 virus Mak, Gannon CK; Cheng, Peter KC; Lau, Stephen SY Journal of Clinical Virology, Vol. 129 https://doi.org/10.1016/j.jcv.2020.104500	journal	August 2020
Diagnostic tool or screening programme? Asymptomatic testing for SARS-CoV-2 needs clear goals and protocols Skittrall, Jordan P.; Fortune, Mary D.; Jalal, Hamid The Lancet Regional Health - Europe, Vol. 1 https://doi.org/10.1016/j.lanepe.2020.100002	journal	February 2021
The Interpretation of SARS-CoV-2 Diagnostic Tests Stites, Edward C.; Wilen, Craig B. Med, Vol. 1, Issue 1 https://doi.org/10.1016/j.medj.2020.08.001	journal	December 2020
SalivaDirect: A simplified and flexible platform to enhance SARS-CoV-2 testing capacity Vogels, Chantal B. F.; Watkins, Anne E.; Harden, Christina A. Med, Vol. 2, Issue 3 https://doi.org/10.1016/j.medj.2020.12.010	journal	March 2021
Contamination of SARS-CoV-2 RT-PCR probes at the oligonucleotide manufacturer Wang, Claire Y. T.; Buckley, Cameron; Bletchly, Cheryl Pathology, Vol. 52, Issue 7 https://doi.org/10.1016/j.pathol.2020.08.002	journal	December 2020
First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community Ahmed, Warish; Angel, Nicola; Edson, Janette Science of The Total Environment, Vol. 728 https://doi.org/10.1016/j.scitotenv.2020.138764	journal	August 2020
COVID-19 containment on a college campus via wastewater-based epidemiology, targeted clinical testing and an intervention Betancourt, Walter Q.; Schmitz, Bradley W.; Innes, Gabriel K. Science of The Total Environment, Vol. 779 https://doi.org/10.1016/j.scitotenv.2021.146408	journal	July 2021
SARS-CoV-2 RNA in Wastewater Settled Solids Is Associated with COVID-19 Cases in a Large Urban Sewershed Graham, Katherine E.; Loeb, Stephanie K.; Wolfe, Marlene K. Environmental Science & Technology, Vol. 55, Issue 1 https://doi.org/10.1021/acs.est.0c06191	journal	December 2020
Presence of SARS-Coronavirus-2 RNA in Sewage and Correlation with Reported COVID-19 Prevalence in the Early Stage of the Epidemic in The Netherlands Medema, Gertjan; Heijnen, Leo; Elsinga, Goffe Environmental Science & Technology Letters, Vol. 7, Issue 7 https://doi.org/10.1021/acs.estlett.0c00357	journal	May 2020
Coronavirus and the race to distribute reliable diagnostics Sheridan, Cormac Nature Biotechnology, Vol. 38, Issue 4 https://doi.org/10.1038/d41587-020-00002-2	journal	February 2020
Establishment and cryptic transmission of Zika virus in Brazil and the Americas Faria, N. R.; Quick, J.; Claro, I. M. Nature, Vol. 546, Issue 7658 https://doi.org/10.1038/nature22401	journal	May 2017
Towards a genomics-informed, real-time, global pathogen surveillance system Gardy, Jennifer L.; Loman, Nicholas J. Nature Reviews Genetics, Vol. 19, Issue 1 https://doi.org/10.1038/nrg.2017.88	journal	November 2017
Tracking the COVID-19 pandemic in Australia using genomics Seemann, Torsten; Lane, Courtney R.; Sherry, Norelle L. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-18314-x	journal	September 2020
SARS-CoV-2 seroprevalence and neutralizing activity in donor and patient blood Ng, Dianna L.; Goldgof, Gregory M.; Shy, Brian R. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-18468-8	journal	September 2020
Full genome viral sequences inform patterns of SARS-CoV-2 spread into and within Israel Miller, Danielle; Martin, Michael A.; Harel, Noam Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-19248-0	journal	November 2020
No evidence for increased transmissibility from recurrent mutations in SARS-CoV-2 van Dorp, Lucy; Richard, Damien; Tan, Cedric C. S. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-19818-2	journal	November 2020
Anomalous collapses of Nares Strait ice arches leads to enhanced export of Arctic sea ice Moore, G. W. K.; Howell, S. E. L.; Brady, M. Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-020-20314-w	journal	January 2021
SARS-CoV-2 antibody prevalence in England following the first peak of the pandemic Ward, Helen; Atchison, Christina; Whitaker, Matthew Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21237-w	journal	February 2021
Global supply-chain effects of COVID-19 control measures Guan, Dabo; Wang, Daoping; Hallegatte, Stephane Nature Human Behaviour, Vol. 4, Issue 6 https://doi.org/10.1038/s41562-020-0896-8	journal	June 2020
Diagnostics for SARS-CoV-2 infections Kevadiya, Bhavesh D.; Machhi, Jatin; Herskovitz, Jonathan Nature Materials, Vol. 20, Issue 5 https://doi.org/10.1038/s41563-020-00906-z	journal	February 2021
Phylogenetic interpretation during outbreaks requires caution Villabona-Arenas, Ch. Julián; Hanage, William P.; Tully, Damien C. Nature Microbiology, Vol. 5, Issue 7 https://doi.org/10.1038/s41564-020-0738-5	journal	May 2020
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets Vogels, Chantal B. F.; Brito, Anderson F.; Wyllie, Anne L. Nature Microbiology, Vol. 5, Issue 10 https://doi.org/10.1038/s41564-020-0761-6	journal	July 2020
The trinity of COVID-19: immunity, inflammation and intervention Tay, Matthew Zirui; Poh, Chek Meng; Rénia, Laurent Nature Reviews Immunology, Vol. 20, Issue 6 https://doi.org/10.1038/s41577-020-0311-8	journal	April 2020
Origin and evolution of pathogenic coronaviruses Cui, Jie; Li, Fang; Shi, Zheng-Li Nature Reviews Microbiology, Vol. 17, Issue 3 https://doi.org/10.1038/s41579-018-0118-9	journal	December 2018
Considerations for diagnostic COVID-19 tests Vandenberg, Olivier; Martiny, Delphine; Rochas, Olivier Nature Reviews Microbiology, Vol. 19, Issue 3 https://doi.org/10.1038/s41579-020-00461-z	journal	October 2020
Underdetection of cases of COVID-19 in France threatens epidemic control Pullano, Giulia; Di Domenico, Laura; Sabbatini, Chiara E. Nature, Vol. 590, Issue 7844 https://doi.org/10.1038/s41586-020-03095-6	journal	December 2020
A new coronavirus associated with human respiratory disease in China Wu, Fan; Zhao, Su; Yu, Bin Nature, Vol. 579, Issue 7798 https://doi.org/10.1038/s41586-020-2008-3	journal	February 2020
A pneumonia outbreak associated with a new coronavirus of probable bat origin Zhou, Peng; Yang, Xing-Lou; Wang, Xian-Guang Nature, Vol. 579, Issue 7798 https://doi.org/10.1038/s41586-020-2012-7	journal	February 2020
Virological assessment of hospitalized patients with COVID-2019 Wölfel, Roman; Corman, Victor M.; Guggemos, Wolfgang Nature, Vol. 581, Issue 7809 https://doi.org/10.1038/s41586-020-2196-x	journal	April 2020
Estimating the effects of non-pharmaceutical interventions on COVID-19 in Europe Flaxman, Seth; Mishra, Swapnil; Gandy, Axel Nature, Vol. 584, Issue 7820 https://doi.org/10.1038/s41586-020-2405-7	journal	June 2020
Suppression of a SARS-CoV-2 outbreak in the Italian municipality of Vo’ Lavezzo, Enrico; Franchin, Elisa; Ciavarella, Constanze Nature, Vol. 584, Issue 7821 https://doi.org/10.1038/s41586-020-2488-1	journal	June 2020
A pooled testing strategy for identifying SARS-CoV-2 at low prevalence Mutesa, Leon; Ndishimye, Pacifique; Butera, Yvan Nature, Vol. 589, Issue 7841 https://doi.org/10.1038/s41586-020-2885-5	journal	October 2020
Spike mutation D614G alters SARS-CoV-2 fitness Plante, Jessica A.; Liu, Yang; Liu, Jianying Nature https://doi.org/10.1038/s41586-020-2895-3	journal	October 2020
Detection of a SARS-CoV-2 variant of concern in South Africa Tegally, Houriiyah; Wilkinson, Eduan; Giovanetti, Marta Nature, Vol. 592, Issue 7854 https://doi.org/10.1038/s41586-021-03402-9	journal	March 2021
CRISPR–Cas12-based detection of SARS-CoV-2 Broughton, James P.; Deng, Xianding; Yu, Guixia Nature Biotechnology, Vol. 38, Issue 7 https://doi.org/10.1038/s41587-020-0513-4	journal	April 2020
Diagnostics and the coronavirus: don’t let the standards slip Page, Mark; Almond, Neil; Rose, Nicola J. Nature Biotechnology, Vol. 38, Issue 6 https://doi.org/10.1038/s41587-020-0558-4	journal	May 2020
Pandemic preparedness in Taiwan Wu, Wei-Kai; Liou, Jyh-Ming; Hsu, Cheng-Chih Nature Biotechnology, Vol. 38, Issue 8 https://doi.org/10.1038/s41587-020-0630-0	journal	July 2020
The COVID-19 XPRIZE and the need for scalable, fast, and widespread testing MacKay, Matthew J.; Hooker, Anna C.; Afshinnekoo, Ebrahim Nature Biotechnology, Vol. 38, Issue 9 https://doi.org/10.1038/s41587-020-0655-4	journal	August 2020
Evaluation of SARS-CoV-2 serology assays reveals a range of test performance Whitman, Jeffrey D.; Hiatt, Joseph; Mowery, Cody T. Nature Biotechnology, Vol. 38, Issue 10 https://doi.org/10.1038/s41587-020-0659-0	journal	August 2020
Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamics Peccia, Jordan; Zulli, Alessandro; Brackney, Doug E. Nature Biotechnology, Vol. 38, Issue 10 https://doi.org/10.1038/s41587-020-0684-z	journal	September 2020
Antibody responses to SARS-CoV-2 in patients with COVID-19 Long, Quan-Xin; Liu, Bai-Zhong; Deng, Hai-Jun Nature Medicine, Vol. 26, Issue 6 https://doi.org/10.1038/s41591-020-0897-1	journal	April 2020
A serological assay to detect SARS-CoV-2 seroconversion in humans Amanat, Fatima; Stadlbauer, Daniel; Strohmeier, Shirin Nature Medicine, Vol. 26, Issue 7 https://doi.org/10.1038/s41591-020-0913-5	journal	May 2020
Clinical and immunological assessment of asymptomatic SARS-CoV-2 infections Long, Quan-Xin; Tang, Xiao-Jun; Shi, Qiu-Lin Nature Medicine https://doi.org/10.1038/s41591-020-0965-6	journal	June 2020
Rapid SARS-CoV-2 whole-genome sequencing and analysis for informed public health decision-making in the Netherlands Oude Munnink, Bas B.; Nieuwenhuijse, David F.; Stein, Mart Nature Medicine, Vol. 26, Issue 9 https://doi.org/10.1038/s41591-020-0997-y	journal	July 2020
Revealing COVID-19 transmission in Australia by SARS-CoV-2 genome sequencing and agent-based modeling Rockett, Rebecca J.; Arnott, Alicia; Lam, Connie Nature Medicine, Vol. 26, Issue 9 https://doi.org/10.1038/s41591-020-1000-7	journal	July 2020
A cross-country database of COVID-19 testing Hasell, Joe; Mathieu, Edouard; Beltekian, Diana Scientific Data, Vol. 7, Issue 1 https://doi.org/10.1038/s41597-020-00688-8	journal	October 2020
False Positive Alarm Frasier, Sarah Lewin Scientific American, Vol. 323, Issue 1 https://doi.org/10.1038/scientificamerican0720-12	journal	July 2020
Aerosol and Surface Stability of SARS-CoV-2 as Compared with SARS-CoV-1 van Doremalen, Neeltje; Bushmaker, Trenton; Morris, Dylan H. New England Journal of Medicine, Vol. 382, Issue 16 https://doi.org/10.1056/NEJMc2004973	journal	April 2020
Rapid Response to an Outbreak in Qingdao, China Xing, Yuhan; Wong, Gary W. K.; Ni, Wei New England Journal of Medicine, Vol. 383, Issue 23 https://doi.org/10.1056/NEJMc2032361	journal	December 2020
Asymptomatic Transmission, the Achilles’ Heel of Current Strategies to Control Covid-19 Gandhi, Monica; Yokoe, Deborah S.; Havlir, Diane V. New England Journal of Medicine, Vol. 382, Issue 22 https://doi.org/10.1056/NEJMe2009758	journal	May 2020
Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus–Infected Pneumonia Li, Qun; Guan, Xuhua; Wu, Peng New England Journal of Medicine, Vol. 382, Issue 13 https://doi.org/10.1056/NEJMoa2001316	journal	March 2020
Spread of SARS-CoV-2 in the Icelandic Population Gudbjartsson, Daniel F.; Helgason, Agnar; Jonsson, Hakon New England Journal of Medicine, Vol. 382, Issue 24 https://doi.org/10.1056/NEJMoa2006100	journal	June 2020
Humoral Immune Response to SARS-CoV-2 in Iceland Gudbjartsson, Daniel F.; Norddahl, Gudmundur L.; Melsted, Pall New England Journal of Medicine, Vol. 383, Issue 18 https://doi.org/10.1056/NEJMoa2026116	journal	October 2020
Covid-19 Molecular Diagnostic Testing — Lessons Learned Shuren, Jeffrey; Stenzel, Timothy New England Journal of Medicine, Vol. 383, Issue 17 https://doi.org/10.1056/NEJMp2023830	journal	October 2020
Rethinking Covid-19 Test Sensitivity — A Strategy for Containment Mina, Michael J.; Parker, Roy; Larremore, Daniel B. New England Journal of Medicine, Vol. 383, Issue 22 https://doi.org/10.1056/NEJMp2025631	journal	November 2020
The Missing Piece — SARS-CoV-2 Testing and School Reopening Rafiei, Yasmin; Mello, Michelle M. New England Journal of Medicine, Vol. 383, Issue 23 https://doi.org/10.1056/NEJMp2028209	journal	December 2020
Rapid Scaling Up of Covid-19 Diagnostic Testing in the United States — The NIH RADx Initiative Tromberg, Bruce J.; Schwetz, Tara A.; Pérez-Stable, Eliseo J. New England Journal of Medicine, Vol. 383, Issue 11 https://doi.org/10.1056/NEJMsr2022263	journal	September 2020
Impact of international travel and border control measures on the global spread of the novel 2019 coronavirus outbreak Wells, Chad R.; Sah, Pratha; Moghadas, Seyed M. Proceedings of the National Academy of Sciences, Vol. 117, Issue 13 https://doi.org/10.1073/pnas.2002616117	journal	March 2020
Phylogenetic network analysis of SARS-CoV-2 genomes Forster, Peter; Forster, Lucy; Renfrew, Colin Proceedings of the National Academy of Sciences, Vol. 117, Issue 17 https://doi.org/10.1073/pnas.2004999117	journal	April 2020
Identifying airborne transmission as the dominant route for the spread of COVID-19 Zhang, Renyi; Li, Yixin; Zhang, Annie L. Proceedings of the National Academy of Sciences, Vol. 117, Issue 26 https://doi.org/10.1073/pnas.2009637117	journal	June 2020
Nextstrain: real-time tracking of pathogen evolution Hadfield, James; Megill, Colin; Bell, Sidney M. Bioinformatics, Vol. 34, Issue 23 https://doi.org/10.1093/bioinformatics/bty407	journal	May 2018
Clinical Performance of Two SARS-CoV-2 Serologic Assays Tang, Mei San; Hock, Karl G.; Logsdon, Nicole M. Clinical Chemistry, Vol. 66, Issue 8 https://doi.org/10.1093/clinchem/hvaa120	journal	June 2020
Are Seroprevalence Estimates for Severe Acute Respiratory Syndrome Coronavirus 2 Biased? Takahashi, Saki; Greenhouse, Bryan; Rodríguez-Barraquer, Isabel The Journal of Infectious Diseases, Vol. 222, Issue 11 https://doi.org/10.1093/infdis/jiaa523	journal	August 2020
The promise of direct-to-consumer COVID-19 testing: ethical and regulatory issues Kalokairinou, Louiza; Zettler, Patricia J.; Nagappan, Ashwini Journal of Law and the Biosciences, Vol. 7, Issue 1 https://doi.org/10.1093/jlb/lsaa069	journal	January 2020
Loop-mediated isothermal amplification of DNA Notomi, T. Nucleic Acids Research, Vol. 28, Issue 12 https://doi.org/10.1093/nar/28.12.e63	journal	June 2000
Cold-chain food contamination as the possible origin of COVID-19 resurgence in Beijing Pang, Xinghuo; Ren, Lili; Wu, Shuangsheng National Science Review, Vol. 7, Issue 12 https://doi.org/10.1093/nsr/nwaa264	journal	October 2020
Efficient high-throughput SARS-CoV-2 testing to detect asymptomatic carriers Shental, Noam; Levy, Shlomia; Wuvshet, Vered Science Advances, Vol. 6, Issue 37 https://doi.org/10.1126/sciadv.abc5961	journal	August 2020
Test sensitivity is secondary to frequency and turnaround time for COVID-19 screening Larremore, Daniel B.; Wilder, Bryan; Lester, Evan Science Advances, Vol. 7, Issue 1 https://doi.org/10.1126/sciadv.abd5393	journal	November 2020
Genomic surveillance elucidates Ebola virus origin and transmission during the 2014 outbreak Gire, S. K.; Goba, A.; Andersen, K. G. Science, Vol. 345, Issue 6202 https://doi.org/10.1126/science.1259657	journal	August 2014
The effect of travel restrictions on the spread of the 2019 novel coronavirus (COVID-19) outbreak Chinazzi, Matteo; Davis, Jessica T.; Ajelli, Marco Science, Vol. 368, Issue 6489 https://doi.org/10.1126/science.aba9757	journal	March 2020
Substantial undocumented infection facilitates the rapid dissemination of novel coronavirus (SARS-CoV-2) Li, Ruiyun; Pei, Sen; Chen, Bin Science, Vol. 368, Issue 6490 https://doi.org/10.1126/science.abb3221	journal	March 2020
The effect of human mobility and control measures on the COVID-19 epidemic in China Kraemer, Moritz U. G.; Yang, Chia-Hung; Gutierrez, Bernardo Science, Vol. 368, Issue 6490 https://doi.org/10.1126/science.abb4218	journal	March 2020
Projecting the transmission dynamics of SARS-CoV-2 through the postpandemic period Kissler, Stephen M.; Tedijanto, Christine; Goldstein, Edward Science, Vol. 368, Issue 6493 https://doi.org/10.1126/science.abb5793	journal	April 2020
Quantifying SARS-CoV-2 transmission suggests epidemic control with digital contact tracing Ferretti, Luca; Wymant, Chris; Kendall, Michelle Science, Vol. 368, Issue 6491 https://doi.org/10.1126/science.abb6936	journal	March 2020
Changes in contact patterns shape the dynamics of the COVID-19 outbreak in China Zhang, Juanjuan; Litvinova, Maria; Liang, Yuxia Science, Vol. 368, Issue 6498 https://doi.org/10.1126/science.abb8001	journal	April 2020
Genomic surveillance reveals multiple introductions of SARS-CoV-2 into Northern California Deng, Xianding; Gu, Wei; Federman, Scot Science, Vol. 369, Issue 6503 https://doi.org/10.1126/science.abb9263	journal	June 2020
Cryptic transmission of SARS-CoV-2 in Washington state Bedford, Trevor; Greninger, Alexander L.; Roychoudhury, Pavitra Science, Vol. 370, Issue 6516 https://doi.org/10.1126/science.abc0523	journal	September 2020
Antibody surveys suggesting vast undercount of coronavirus infections may be unreliable Vogel, Gretchen Science https://doi.org/10.1126/science.abc3831	journal	April 2020
The emergence of SARS-CoV-2 in Europe and North America Worobey, Michael; Pekar, Jonathan; Larsen, Brendan B. Science, Vol. 370, Issue 6516 https://doi.org/10.1126/science.abc8169	journal	September 2020
Phylogenetic analysis of SARS-CoV-2 in Boston highlights the impact of superspreading events Lemieux, Jacob E.; Siddle, Katherine J.; Shaw, Bennett M. Science, Vol. 371, Issue 6529 https://doi.org/10.1126/science.abe3261	journal	December 2020
Transmission of SARS-CoV-2 on mink farms between humans and mink and back to humans Oude Munnink, Bas B.; Sikkema, Reina S.; Nieuwenhuijse, David F. Science, Vol. 371, Issue 6525 https://doi.org/10.1126/science.abe5901	journal	November 2020
The impact of population-wide rapid antigen testing on SARS-CoV-2 prevalence in Slovakia Pavelka, Martin; Van-Zandvoort, Kevin; Abbott, Sam Science, Vol. 372, Issue 6542 https://doi.org/10.1126/science.abf9648	journal	March 2021
Estimated transmissibility and impact of SARS-CoV-2 lineage B.1.1.7 in England Davies, Nicholas G.; Abbott, Sam; Barnard, Rosanna C. Science, Vol. 372, Issue 6538 https://doi.org/10.1126/science.abg3055	journal	March 2021
Estimation of polio infection prevalence from environmental surveillance data Berchenko, Yakir; Manor, Yossi; Freedman, Laurence S. Science Translational Medicine, Vol. 9, Issue 383 https://doi.org/10.1126/scitranslmed.aaf6786	journal	March 2017
A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples Dao Thi, Viet Loan; Herbst, Konrad; Boerner, Kathleen Science Translational Medicine, Vol. 12, Issue 556 https://doi.org/10.1126/scitranslmed.abc7075	journal	July 2020
Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests Barak, Netta; Ben-Ami, Roni; Sido, Tal Science Translational Medicine, Vol. 13, Issue 589 https://doi.org/10.1126/scitranslmed.abf2823	journal	February 2021
Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit Nalla, Arun K.; Casto, Amanda M.; Huang, Meei-Li W. Journal of Clinical Microbiology, Vol. 58, Issue 6 https://doi.org/10.1128/JCM.00557-20	journal	April 2020
Evaluation of a Rapid Diagnostic Assay for Detection of SARS-CoV-2 Antigen in Nasopharyngeal Swabs Lambert-Niclot, Sidonie; Cuffel, Alexis; Le Pape, Samuel Journal of Clinical Microbiology, Vol. 58, Issue 8 https://doi.org/10.1128/JCM.00977-20	journal	July 2020
A Recurrent Mutation at Position 26340 of SARS-CoV-2 Is Associated with Failure of the E Gene Quantitative Reverse Transcription-PCR Utilized in a Commercial Dual-Target Diagnostic Assay Artesi, Maria; Bontems, Sébastien; Göbbels, Paul Journal of Clinical Microbiology, Vol. 58, Issue 10 https://doi.org/10.1128/JCM.01598-20	journal	September 2020
Genomic Characterization of a Novel SARS-CoV-2 Lineage from Rio de Janeiro, Brazil Voloch, Carolina M.; da Silva Francisco, Ronaldo; de Almeida, Luiz G. P. Journal of Virology, Vol. 95, Issue 10 https://doi.org/10.1128/JVI.00119-21	journal	April 2021
Monitoring COVID-19 Transmission Risks by Quantitative Real-Time PCR Tracing of Droplets in Hospital and Living Environments Piana, Andrea; Colucci, Maria Eugenia; Valeriani, Federica mSphere, Vol. 6, Issue 1 https://doi.org/10.1128/mSphere.01070-20	journal	February 2021
Covid-19 mass testing programmes Raffle, Angela E.; Pollock, Allyson M.; Harding-Edgar, Louisa BMJ https://doi.org/10.1136/bmj.m3262	journal	August 2020
Covid-19: Mass population testing is rolled out in Liverpool Iacobucci, Gareth BMJ https://doi.org/10.1136/bmj.m4268	journal	November 2020
Covid-19: Lateral flow tests miss over half of cases, Liverpool pilot data show Wise, Jacqui BMJ https://doi.org/10.1136/bmj.m4848	journal	December 2020
Asymptomatic transmission of covid-19 Pollock, Allyson M.; Lancaster, James BMJ https://doi.org/10.1136/bmj.m4851	journal	December 2020
Information and rational decision-making: explanations to patients and citizens about personal risk of COVID-19 McCartney, Margaret; Sullivan, Frank; Heneghan, Carl BMJ Evidence-Based Medicine https://doi.org/10.1136/bmjebm-2020-111541	journal	October 2020
Reconstructing the early global dynamics of under-ascertained COVID-19 cases and infections Russell, Timothy W.; Golding, Nick; Hellewell, Joel BMC Medicine, Vol. 18, Issue 1 https://doi.org/10.1186/s12916-020-01790-9	journal	October 2020
Response to COVID-19 in South Korea and implications for lifting stringent interventions Dighe, Amy; Cattarino, Lorenzo; Cuomo-Dannenburg, Gina BMC Medicine, Vol. 18, Issue 1 https://doi.org/10.1186/s12916-020-01791-8	journal	October 2020
DNA Detection Using Recombination Proteins Piepenburg, Olaf; Williams, Colin H.; Stemple, Derek L. PLoS Biology, Vol. 4, Issue 7 https://doi.org/10.1371/journal.pbio.0040204	journal	June 2006
Superspreading events in the transmission dynamics of SARS-CoV-2: Opportunities for interventions and control Althouse, Benjamin M.; Wenger, Edward A.; Miller, Joel C. PLOS Biology, Vol. 18, Issue 11 https://doi.org/10.1371/journal.pbio.3000897	journal	November 2020
SARS-CoV-2 infection in farmed minks, the Netherlands, April and May 2020 Oreshkova, Nadia; Molenaar, Robert Jan; Vreman, Sandra Eurosurveillance, Vol. 25, Issue 23 https://doi.org/10.2807/1560-7917.ES.2020.25.23.2001005	journal	June 2020
SARS-CoV-2 samples may escape detection because of a single point mutation in the N gene Ziegler, Katharina; Steininger, Philipp; Ziegler, Renate Eurosurveillance, Vol. 25, Issue 39 https://doi.org/10.2807/1560-7917.ES.2020.25.39.2001650	journal	October 2020
Estimating the false-negative test probability of SARS-CoV-2 by RT-PCR Wikramaratna, Paul S.; Paton, Robert S.; Ghafari, Mahan Eurosurveillance, Vol. 25, Issue 50 https://doi.org/10.2807/1560-7917.ES.2020.25.50.2000568	journal	December 2020
Complexity of the Basic Reproduction Number (R ₀ ) Delamater, Paul L.; Street, Erica J.; Leslie, Timothy F. Emerging Infectious Diseases, Vol. 25, Issue 1 https://doi.org/10.3201/eid2501.171901	journal	January 2019
Delayed Laboratory Response to COVID-19 Caused by Molecular Diagnostic Contamination Mögling, Ramona; Meijer, Adam; Berginc, Natasa Emerging Infectious Diseases, Vol. 26, Issue 8 https://doi.org/10.3201/eid2608.201843	journal	August 2020
Coronavirus Disease Exposure and Spread from Nightclubs, South Korea Kang, Cho Ryok; Lee, Jin Yong; Park, Yoojin Emerging Infectious Diseases, Vol. 26, Issue 10 https://doi.org/10.3201/eid2610.202573	journal	October 2020
On Pilot Massive COVID-19 Testing by Antigen Tests in Europe. Case Study: Slovakia Frnda, Jaroslav; Durica, Marek Infectious Disease Reports, Vol. 13, Issue 1 https://doi.org/10.3390/idr13010007	journal	January 2021
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Lessons Learnt From the COVID-19 Pandemic

Journal Article · Sun Aug 01 20:00:00 EDT 2021 · Frontiers in Public Health · OSTI ID:1811574

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