The distribution, diversity and function of predominant Thermoproteales in high‐temperature environments of Yellowstone National Park

Jay, Zackary J.; Beam, Jacob P.; Kozubal, Mark A.; Jennings, Ryan deM.; Rusch, Douglas B.; Inskeep, William P.

doi:10.1111/1462-2920.13366

Title: The distribution, diversity and function of predominant Thermoproteales in high‐temperature environments of Yellowstone National Park

Journal Article · Mon May 30 00:00:00 EDT 2016 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.13366· OSTI ID:1787073

Jay, Zackary J. ^[1]; Beam, Jacob P. ^[1]; Kozubal, Mark A. ^[1]; Jennings, Ryan deM. ^[1]; Rusch, Douglas B. ^[2]; Inskeep, William P. ^[1]

Department of Land Resources and Environmental Sciences and Thermal Biology Institute Montana State University Bozeman MT 59175‐3120 USA
Center for Genomics and Bioinformatics, Indiana University Bloomington IN USA

Summary High‐temperature environments (> 70°C) contain diverse and abundant members of the crenarchaeal order Thermoproteales. However, a comprehensive study of the distribution and function of diverse members of this group across different habitat types has not been conducted. Consequently, the goals of this study were to determine the distribution of different Thermoproteales genera across geochemically distinct geothermal habitats of Yellowstone National Park, and to identify key functional attributes of major genera that correlate with environmental parameters. Curated sequence assemblies belonging to five genera were characterized in replicate samples of 11 high‐temperature communities ranging in pH from 3 to 9. Thermocladium , Vulcanisaeta and Caldivirga spp. were the primary Thermoproteales populations present in low pH (pH < 5) habitats, whereas Thermoproteus populations were found in mildly‐acidic (pH 5–6) sulfur sediments, and Pyrobaculum populations were confined to higher pH (pH > 6) sulfur sediments and/or filamentous ‘streamer’ communities. Metabolic reconstruction and comparative genomics among assemblies show that these populations are primarily chemoorganotrophs that utilize different electron acceptors depending on geochemical conditions. The presence of potential CO ₂ fixation pathways in some Thermoproteales populations appears to be linked with NiFe hydrogenases, which combined with high levels of H ₂ in many sulfidic systems, may provide the energy required to fix inorganic C.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: 112443; 254840; CSP 787081; CSP 701

OSTI ID:: 1787073

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Vol. 18 Journal Issue: 12; ISSN 1462-2912

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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