Thermophilic microbial deconstruction and conversion of natural and transgenic lignocellulose

Bing, Ryan G.; Sulis, Daniel B.; Wang, Jack P.; Adams, Michael W. W.; Kelly, Robert M.

doi:10.1111/1758-2229.12943

Thermophilic microbial deconstruction and conversion of natural and transgenic lignocellulose

Journal Article · Tue Mar 16 00:00:00 EDT 2021 · Environmental Microbiology Reports

DOI:https://doi.org/10.1111/1758-2229.12943· OSTI ID:1784353

Bing, Ryan G. ^[1]; Sulis, Daniel B. ^[2]; Wang, Jack P. ^[2]; Adams, Michael W. W. ^[3]; ^[1]

Department of Chemical and Biomolecular Engineering North Carolina State University Raleigh NC 27695 USA
Department of Forestry and Environmental Resources North Carolina State University Raleigh NC 27695 USA
Department of Biochemistry and Molecular Biology University of Georgia Athens GA 30602 USA

Summary

The potential to convert renewable plant biomasses into fuels and chemicals by microbial processes presents an attractive, less environmentally intense alternative to conventional routes based on fossil fuels. This would best be done with microbes that natively deconstruct lignocellulose and concomitantly form industrially relevant products, but these two physiological and metabolic features are rarely and simultaneously observed in nature. Genetic modification of both plant feedstocks and microbes can be used to increase lignocellulose deconstruction capability and generate industrially relevant products. Separate efforts on plants and microbes are ongoing, but these studies lack a focus on optimal, complementary combinations of these disparate biological systems to obtain a convergent technology. Improving genetic tools for plants have given rise to the generation of low‐lignin lines that are more readily solubilized by microorganisms. Most focus on the microbiological front has involved thermophilic bacteria from the genera Caldicellulosiruptor and Clostridium , given their capacity to degrade lignocellulose and to form bio‐products through metabolic engineering strategies enabled by ever‐improving molecular genetics tools. Bioengineering plant properties to better fit the deconstruction capabilities of candidate consolidated bioprocessing microorganisms has potential to achieve the efficient lignocellulose deconstruction needed for industrial relevance.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0019391

OSTI ID:: 1784353

Alternate ID(s):: OSTI ID: 1853181

Journal Information:: Environmental Microbiology Reports, Journal Name: Environmental Microbiology Reports Journal Issue: 3 Vol. 13; ISSN 1758-2229

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United States

Language:: English

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