Dimer Asymmetry and Light Activation Mechanism in Brucella Blue-Light Sensor Histidine Kinase

Rinaldi, Jimena; Fernández, Ignacio; Shin, Heewhan; Sycz, Gabriela; Gunawardana, Semini; Kumarapperuma, Indika; Paz, Juan M.; Otero, Lisandro H.; Cerutti, María L.; Zorreguieta, Ángeles; Ren, Zhong; Klinke, Sebastián; Yang, Xiaojing; Goldbaum, Fernando A.

doi:10.1128/mbio.00264-21

Dimer Asymmetry and Light Activation Mechanism in Brucella Blue-Light Sensor Histidine Kinase

Journal Article · Tue Apr 20 00:00:00 EDT 2021 · mBio (Online)

DOI:https://doi.org/10.1128/mbio.00264-21· OSTI ID:1783844

Rinaldi, Jimena ^[1]; Fernández, Ignacio ^[1]; Shin, Heewhan ^[2]; Sycz, Gabriela ^[1]; Gunawardana, Semini ^[2]; Kumarapperuma, Indika ^[2]; Paz, Juan M. ^[1]; Otero, Lisandro H. ^[3]; Cerutti, María L. ^[3]; Zorreguieta, Ángeles ^[1]; Ren, Zhong ^[2]; ^[3]; ^[2]; ^[3]

IIBBA-CONICET (Argentina)
Univ. of Illinois, Chicago, IL (United States)
IIBBA-CONICET (Argentina); Plataforma Argentina de Biología Estructural y Metabolómica (PLABEM) (Argentina)

The ability to sense and respond to environmental cues is essential for adaptation and survival in living organisms. In bacteria, this process is accomplished by multidomain sensor histidine kinases that undergo autophosphorylation in response to specific stimuli, thereby triggering downstream signaling cascades. However, the molecular mechanism of allosteric activation is not fully understood in these important sensor proteins. Here, we report the full-length crystal structure of a blue light photoreceptor LOV histidine kinase (LOV-HK) involved in light-dependent virulence modulation in the pathogenic bacterium Brucella abortus. Joint analyses of dark and light structures determined in different signaling states have shown that LOV-HK transitions from a symmetric dark structure to a highly asymmetric light state. The initial local and subtle structural signal originated in the chromophore-binding LOV domain alters the dimer asymmetry via a coiled-coil rotary switch and helical bending in the helical spine. These amplified structural changes result in enhanced conformational flexibility and large-scale rearrangements that facilitate the phosphoryl transfer reaction in the HK domain.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1783844

Journal Information:: mBio (Online), Journal Name: mBio (Online) Journal Issue: 2 Vol. 12; ISSN 2150-7511

Publisher:: American Society for Microbiology (ASM)Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
crystallography
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Dimer Asymmetry and Light Activation Mechanism in Brucella Blue-Light Sensor Histidine Kinase

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