Rapid Response of Nitrogen Cycling Gene Transcription to Labile Carbon Amendments in a Soil Microbial Community

Chuckran, Peter F.; Fofanov, Viacheslav; Hungate, Bruce A.; Morrissey, Ember M.; Schwartz, Egbert; Walkup, Jeth; Dijkstra, Paul; McClure, ed., Ryan

doi:10.1128/mSystems.00161-21

Title: Rapid Response of Nitrogen Cycling Gene Transcription to Labile Carbon Amendments in a Soil Microbial Community

Journal Article · Tue Jun 29 00:00:00 EDT 2021 · mSystems

DOI:https://doi.org/10.1128/mSystems.00161-21· OSTI ID:1782385

^[1]; Fofanov, Viacheslav ^[2];

^[1]; Morrissey, Ember M. ^[3]; Schwartz, Egbert ^[1]; Walkup, Jeth ^[3]; Dijkstra, Paul ^[1]; McClure, ed., Ryan

Center for Ecosystem Science and Society (ECOSS), Northern Arizona University, Flagstaff, Arizona, USA, Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, USA
Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, USA, School of Informatics, Computing and Cyber Systems, Northern Arizona University, Flagstaff, Arizona, USA
Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia, USA

Episodic inputs of labile carbon (C) to soil can rapidly stimulate nitrogen (N) immobilization by soil microorganisms. However, the transcriptional patterns that underlie this process remain unclear. In order to better understand the regulation of N cycling in soil microbial communities, we conducted a 48-h laboratory incubation with agricultural soil where we stimulated the uptake of inorganic N by amending the soil with glucose. We analyzed the metagenome and metatranscriptome of the microbial communities at four time points that corresponded with changes in N availability. The relative abundances of genes remained largely unchanged throughout the incubation. In contrast, glucose addition rapidly increased the transcription of genes encoding ammonium and nitrate transporters, enzymes responsible for N assimilation into biomass, and genes associated with the N regulatory network. This upregulation coincided with an increase in transcripts associated with glucose breakdown and oxoglutarate production, demonstrating a connection between C and N metabolism. When concentrations of ammonium were low, we observed a transient upregulation of genes associated with the nitrogen-fixing enzyme nitrogenase. Transcripts for nitrification and denitrification were downregulated throughout the incubation, suggesting that dissimilatory transformations of N may be suppressed in response to labile C inputs in these soils. These results demonstrate that soil microbial communities can respond rapidly to changes in C availability by drastically altering the transcription of N cycling genes.

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Research Organization:: Univ. of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SCW1632; AC02-05CH11231

OSTI ID:: 1782385

Alternate ID(s):: OSTI ID: 1816183

Journal Information:: mSystems, Journal Name: mSystems Vol. 6 Journal Issue: 3; ISSN 2379-5077

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Rapid Response of Nitrogen Cycling Gene Transcription to Labile Carbon Amendments in a Soil Microbial Community

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