The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

Negretti, Nicholas M.; Gourley, Christopher R.; Talukdar, Prabhat K.; Clair, Geremy; Klappenbach, Courtney M.; Lauritsen, Cody J.; Adkins, Joshua N.; Konkel, Michael E.

doi:10.1038/s41467-021-21579-5

The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

Journal Article · Fri Feb 26 00:00:00 EST 2021 · Nature Communications

DOI:https://doi.org/10.1038/s41467-021-21579-5· OSTI ID:1781820

^[1]; Gourley, Christopher R. ^[1]; ^[1]; ^[2]; ^[1]; Lauritsen, Cody J. ^[1]; ^[2]; ^[1]

Washington State Univ., Pullman, WA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Campylobacter jejuni is a foodborne pathogen that binds to and invades the epithelial cells lining the human intestinal tract. Maximal invasion of host cells by C. jejuni requires cell binding as well as delivery of the Cia proteins (Campylobacter invasion antigens) to the host cell cytosol via the flagellum. Here, we show that CiaD binds to the host cell protein IQGAP1 (a Ras GTPase-activating-like protein), thus displacing RacGAP1 from the IQGAP1 complex. This, in turn, leads to the unconstrained activity of the small GTPase Rac1, which is known to have roles in actin reorganization and internalization of C. jejuni. Our results represent the identification of a host cell protein targeted by a flagellar secreted effector protein and demonstrate that C. jejuni-stimulated Rac signaling is dependent on IQGAP1.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1781820

Report Number(s):: PNNL-SA--152634

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 12; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

References (79)

Structure and evolution of the actin crosslinking proteins Dubreuil, Ronald R. BioEssays, Vol. 13, Issue 5 https://doi.org/10.1002/bies.950130504	journal	May 1991
km23‐1/DYNLRB1 regulation of MEK/ERK signaling and R‐Ras in invasive human colorectal cancer cells Raza, Asif; Pandey, Madhu S.; Jin, Qunyan Cell Biology International, Vol. 44, Issue 1 https://doi.org/10.1002/cbin.11215	journal	August 2019
Self-made frits for nanoscale columns in proteomics Maiolica, Alessio; Borsotti, Dario; Rappsilber, Juri PROTEOMICS, Vol. 5, Issue 15 https://doi.org/10.1002/pmic.200402010	journal	October 2005
Membrance traffic wardens and protein secretion in Gram-negative bacteria Salmond, George P. C.; Reeves, Philip J. Trends in Biochemical Sciences, Vol. 18, Issue 1 https://doi.org/10.1016/0968-0004(93)90080-7	journal	January 1993
Prediction, Assessment and Validation of Protein Interaction Maps in Bacteria Wojcik, Jérôme; Boneca, Ivo G.; Legrain, Pierre Journal of Molecular Biology, Vol. 323, Issue 4 https://doi.org/10.1016/S0022-2836(02)01009-4	journal	November 2002
[34] Ras-Raf interaction: Two-hybrid analysis Vojtek, Anne B.; Hollenberg, Stanley M. Methods in Enzymology https://doi.org/10.1016/S0076-6879(95)55036-4	book	January 1995
Structural comparisons of calponin homology domains: implications for actin binding Bañuelos, Sonia; Saraste, Matti; Carugo, Kristina Djinović Structure, Vol. 6, Issue 11 https://doi.org/10.1016/S0969-2126(98)00141-5	journal	November 1998
Antibodies against gangliosides: a link between preceding infection and immunopathogenesis of GuillainBarré syndrome Schwerer, Beatrix Microbes and Infection, Vol. 4, Issue 3 https://doi.org/10.1016/S1286-4579(02)01550-2	journal	March 2002
Anti-Immunology: Evasion of the Host Immune System by Bacterial and Viral Pathogens Finlay, B. Brett; McFadden, Grant Cell, Vol. 124, Issue 4 https://doi.org/10.1016/j.cell.2006.01.034	journal	February 2006
The effector repertoire of enteropathogenic E. coli: ganging up on the host cell Dean, Paul; Kenny, Brendan Current Opinion in Microbiology, Vol. 12, Issue 1 https://doi.org/10.1016/j.mib.2008.11.006	journal	February 2009
Cell-Penetrating Bacterial Effector Proteins: Better Tools than Targets Rüter, Christian; Schmidt, M. Alexander Trends in Biotechnology, Vol. 35, Issue 2 https://doi.org/10.1016/j.tibtech.2016.08.002	journal	February 2017
Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry Kelly, Ryan T.; Page, Jason S.; Luo, Quanzhou Analytical Chemistry, Vol. 78, Issue 22, p. 7796-7801 https://doi.org/10.1021/ac061133r	journal	November 2006
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Laemmli, U. K. Nature, Vol. 227, Issue 5259, p. 680-685 https://doi.org/10.1038/227680a0	journal	August 1970
A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerization Moreau, Violaine; Frischknecht, Friedrich; Reckmann, Inge Nature Cell Biology, Vol. 2, Issue 7 https://doi.org/10.1038/35017080	journal	June 2000
The protein–protein interaction map of Helicobacter pylori Rain, Jean-Christophe; Selig, Luc; De Reuse, Hilde Nature, Vol. 409, Issue 6817 https://doi.org/10.1038/35051615	journal	January 2001
The fibronectin-binding motif within FlpA facilitates Campylobacter jejuni adherence to host cell and activation of host cell signaling Larson, Charles L.; Samuelson, Derrick R.; Eucker, Tyson P. Emerging Microbes & Infections, Vol. 2, Issue 1 https://doi.org/10.1038/emi.2013.65	journal	January 2013
MS-GF+ makes progress towards a universal database search tool for proteomics Kim, Sangtae; Pevzner, Pavel A. Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms6277	journal	October 2014
Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens Fromont-Racine, Micheline; Rain, Jean-Christophe; Legrain, Pierre Nature Genetics, Vol. 16, Issue 3 https://doi.org/10.1038/ng0797-277	journal	July 1997
Bacterial strategies for overcoming host innate and adaptive immune responses Hornef, Mathias W.; Wick, Mary Jo; Rhen, Mikael Nature Immunology, Vol. 3, Issue 11 https://doi.org/10.1038/ni1102-1033	journal	November 2002
Campylobacter jejuni: molecular biology and pathogenesis Young, Kathryn T.; Davis, Lindsay M.; DiRita, Victor J. Nature Reviews Microbiology, Vol. 5, Issue 9 https://doi.org/10.1038/nrmicro1718	journal	September 2007
Salmonella enterica serotype Typhimurium usurps the scaffold protein IQGAP1 to manipulate Rac1 and MAPK signalling Kim, Hugh; White, Colin D.; Li, Zhigang Biochemical Journal, Vol. 440, Issue 3 https://doi.org/10.1042/BJ20110419	journal	November 2011
Identification and molecular cloning of a gene encoding a fibronectin‐binding protein (CadF) from Campylobacter jejuni Konkel, Michael E.; Garvis, Steven G.; Tipton, Stephanie L. Molecular Microbiology, Vol. 24, Issue 5 https://doi.org/10.1046/j.1365-2958.1997.4031771.x	journal	June 1997
Bacterial secreted proteins are required for the internalization of Campylobacter jejuni into cultured mammalian cells Konkel, Michael E.; Kim, Bong J.; Rivera-Amill, Vanessa Molecular Microbiology, Vol. 32, Issue 4 https://doi.org/10.1046/j.1365-2958.1999.01376.x	journal	May 1999
Salmonella type III secretion-associated chaperones confer secretion-pathway specificity: Type III secretion chaperones Lee, Sang Ho; Galán, Jorge E. Molecular Microbiology, Vol. 51, Issue 2 https://doi.org/10.1046/j.1365-2958.2003.03840.x	journal	January 2004
Yersinia effectors target mammalian signalling pathways Juris, Stephen J.; Shao, Feng; Dixon, Jack E. Cellular Microbiology, Vol. 4, Issue 4 https://doi.org/10.1046/j.1462-5822.2002.00182.x	journal	April 2002
Characterization of the Interactions between the Small GTPase Cdc42 and Its GTPase-activating Proteins and Putative Effectors Zhang, Baolin; Wang, Zhi-Xin; Zheng, Yi Journal of Biological Chemistry, Vol. 272, Issue 35 https://doi.org/10.1074/jbc.272.35.21999	journal	August 1997
Injection of Flagellin into the Host Cell Cytosol by Salmonella enterica Serotype Typhimurium Sun, Yao-Hui; Rolán, Hortensia GarcÁa; Tsolis, Renée M. Journal of Biological Chemistry, Vol. 282, Issue 47 https://doi.org/10.1074/jbc.C700181200	journal	November 2007
Requirement for the Dynein Light Chain km23-1 in a Smad2-dependent Transforming Growth Factor-β Signaling Pathway Jin, Qunyan; Ding, Wei; Mulder, Kathleen M. Journal of Biological Chemistry, Vol. 282, Issue 26 https://doi.org/10.1074/jbc.M609915200	journal	June 2007
IQGAP1 Regulates Salmonella Invasion through Interactions with Actin, Rac1, and Cdc42 Brown, Matthew D.; Bry, Lynn; Li, Zhigang Journal of Biological Chemistry, Vol. 282, Issue 41 https://doi.org/10.1074/jbc.M702537200	journal	August 2007
Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics Fagerberg, Linn; Hallström, Björn M.; Oksvold, Per Molecular & Cellular Proteomics, Vol. 13, Issue 2 https://doi.org/10.1074/mcp.M113.035600	journal	December 2013
A porcine ligated loop model reveals new insight into the host immune response against Campylobacter jejuni Negretti, Nicholas M.; Ye, Yinyin; Malavasi, Lais M. Gut Microbes, Vol. 12, Issue 1 https://doi.org/10.1080/19490976.2020.1814121	journal	September 2020
Manipulation of kinase signaling by bacterial pathogens Krachler, Anne Marie; Woolery, Andrew R.; Orth, Kim Journal of Cell Biology, Vol. 195, Issue 7 https://doi.org/10.1083/jcb.201107132	journal	November 2011
RCP-driven α5β1 recycling suppresses Rac and promotes RhoA activity via the RacGAP1–IQGAP1 complex Jacquemet, Guillaume; Green, David M.; Bridgewater, Rebecca E. Journal of Cell Biology, Vol. 202, Issue 6 https://doi.org/10.1083/jcb.201302041	journal	September 2013
Secretion of the Virulence‐Associated Campylobacter Invasion Antigens from Campylobacter jejuni Requires a Stimulatory Signal Rivera‐Amill, Vanessa; Kim, Bong J.; Seshu, J. The Journal of Infectious Diseases, Vol. 183, Issue 11 https://doi.org/10.1086/320704	journal	June 2001
A Point-Source Outbreak of Campylobacteriosis Associated with Consumption of Raw Milk Korlath, J. A.; Osterholm, M. T.; Judy, L. A. Journal of Infectious Diseases, Vol. 152, Issue 3 https://doi.org/10.1093/infdis/152.3.592	journal	September 1985
Early Colonic Damage and Invasion of Campylobacter jejuni in Experimentally Challenged Infant Macaca mulatta Russell, Robert G.; O'Donnoghue, Michael; Blake, Daniel C. The Journal of Infectious Diseases, Vol. 168, Issue 1 https://doi.org/10.1093/infdis/168.1.210	journal	July 1993
Kinetic and Antigenic Characterization of Altered Protein Synthesis by Campylobacter jejuni during Cultivation with Human Epithelial Cells Konkel, M. E.; Mead, D. J.; Cieplak, W. Journal of Infectious Diseases, Vol. 168, Issue 4 https://doi.org/10.1093/infdis/168.4.948	journal	October 1993
The InterPro database, an integrated documentation resource for protein families, domains and functional sites Apweiler, R. Nucleic Acids Research, Vol. 29, Issue 1 https://doi.org/10.1093/nar/29.1.37	journal	January 2001
CDD: NCBI's conserved domain database Marchler-Bauer, Aron; Derbyshire, Myra K.; Gonzales, Noreen R. Nucleic Acids Research, Vol. 43, Issue D1 https://doi.org/10.1093/nar/gku1221	journal	November 2014
The eukaryotic linear motif resource – 2018 update Gouw, Marc; Michael, Sushama; Sámano-Sánchez, Hugo Nucleic Acids Research, Vol. 46, Issue D1 https://doi.org/10.1093/nar/gkx1077	journal	November 2017
The Pfam protein families database in 2019 El-Gebali, Sara; Mistry, Jaina; Bateman, Alex Nucleic Acids Research, Vol. 47, Issue D1 https://doi.org/10.1093/nar/gky995	journal	October 2018
Type III secretion systems: the bacterial flagellum and the injectisome Diepold, Andreas; Armitage, Judith P. Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 370, Issue 1679 https://doi.org/10.1098/rstb.2015.0020	journal	October 2015
Maximal adherence and invasion of INT 407 cells by Campylobacter jejuni requires the CadF outer-membrane protein and microfilament reorganization Monteville, M. R. Microbiology, Vol. 149, Issue 1 https://doi.org/10.1099/mic.0.25820-0	journal	January 2003
Ca2+ release from host intracellular stores and related signal transduction during Campylobacter jejuni 81-176 internalization into human intestinal cells Hu, Lan; Raybourne, Richard B.; Kopecko, Dennis J. Microbiology, Vol. 151, Issue 9 https://doi.org/10.1099/mic.0.27866-0	journal	September 2005
Campylobacter jejuni activates mitogen-activated protein kinases in Caco-2 cell monolayers and in vitro infected primary human colonic tissue MacCallum, Amanda; Haddock, Graham; Everest, Paul H. Microbiology, Vol. 151, Issue 8 https://doi.org/10.1099/mic.0.27979-0	journal	August 2005
Protein interaction mapping: A Drosophila case study Formstecher, E. Genome Research, Vol. 15, Issue 3 https://doi.org/10.1101/gr.2659105	journal	February 2005
Role of Microfilaments and Microtubules in the Invasion of INT-407 Cells by Campylobacter jejuni Biswas, Debabrata; Itoh, Kikuji; Sasakawa, Chihiro Microbiology and Immunology, Vol. 47, Issue 6 https://doi.org/10.1111/j.1348-0421.2003.tb03372.x	journal	June 2003
FlaC, a protein of Campylobacter jejuni TGH9011 (ATCC43431) secreted through the flagellar apparatus, binds epithelial cells and influences cell invasion: Secreted FlaC influences invasion Song, Y. C.; Jin, S.; Louie, H. Molecular Microbiology, Vol. 53, Issue 2 https://doi.org/10.1111/j.1365-2958.2004.04175.x	journal	June 2004
Identification of a fibronectin-binding domain within the Campylobacter jejuni CadF protein: C. jejuni fibronectin-binding protein Konkel, Michael E.; Christensen, Jeffrey E.; Keech, Amy M. Molecular Microbiology, Vol. 57, Issue 4 https://doi.org/10.1111/j.1365-2958.2005.04744.x	journal	July 2005
Identification and analysis of flagellar coexpressed determinants (Feds) of Campylobacter jejuni involved in colonization: Flagellar coexpressed determinants of C. jejuni Barrero-Tobon, Angelica M.; Hendrixson, David R. Molecular Microbiology, Vol. 84, Issue 2 https://doi.org/10.1111/j.1365-2958.2012.08027.x	journal	March 2012
Role of the small Rho GTPases Rac1 and Cdc42 in host cell invasion of Campylobacter jejuni Krause-Gruszczynska, Malgorzata; Rohde, Manfred; Hartig, Roland Cellular Microbiology, Vol. 9, Issue 10 https://doi.org/10.1111/j.1462-5822.2007.00971.x	journal	October 2007
Salmonella effectors: important players modulating host cell function during infection: Salmonella effectors Agbor, Terence A.; McCormick, Beth A. Cellular Microbiology, Vol. 13, Issue 12 https://doi.org/10.1111/j.1462-5822.2011.01701.x	journal	October 2011
The cooperative action of bacterial fibronectin-binding proteins and secreted proteins promote maximal Campylobacter jejuni invasion of host cells by stimulating membrane ruffling: C. jejuni invasion of epithelial cells Eucker, Tyson P.; Konkel, Michael E. Cellular Microbiology, Vol. 14, Issue 2 https://doi.org/10.1111/j.1462-5822.2011.01714.x	journal	November 2011
Crystal structure of the repetitive segments of spectrin Yan, Y.; Winograd, E.; Viel, A. Science, Vol. 262, Issue 5142 https://doi.org/10.1126/science.8266097	journal	December 1993
Global Epidemiology of Campylobacter Infection Kaakoush, Nadeem O.; Castaño-Rodríguez, Natalia; Mitchell, Hazel M. Clinical Microbiology Reviews, Vol. 28, Issue 3 https://doi.org/10.1128/CMR.00006-15	journal	June 2015
Identification of Campylobacter jejuni Genes Involved in Its Interaction with Epithelial Cells Novik, Veronica; Hofreuter, Dirk; Galán, Jorge E. Infection and Immunity, Vol. 78, Issue 8 https://doi.org/10.1128/IAI.00109-10	journal	June 2010
Heterogeneity of a Campylobacter jejuni Protein That Is Secreted through the Flagellar Filament Poly, Frédéric; Ewing, Cheryl; Goon, Scarlett Infection and Immunity, Vol. 75, Issue 8 https://doi.org/10.1128/IAI.00159-07	journal	May 2007
Host-Pathogen Interactions: Basic Concepts of Microbial Commensalism, Colonization, Infection, and Disease Casadevall, A.; Pirofski, L. -a. Infection and Immunity, Vol. 68, Issue 12 https://doi.org/10.1128/IAI.68.12.6511-6518.2000	journal	December 2000
Secretion of Flagellar Proteins by the Pseudomonas aeruginosa Type III Secretion-Injectisome System Ince, Dilek; Sutterwala, Fayyaz S.; Yahr, Timothy L. Journal of Bacteriology, Vol. 197, Issue 12 https://doi.org/10.1128/JB.00030-15	journal	April 2015
Campylobacter jejuni FlpA Binds Fibronectin and Is Required for Maximal Host Cell Adherence Konkel, M. E.; Larson, C. L.; Flanagan, R. C. Journal of Bacteriology, Vol. 192, Issue 1 https://doi.org/10.1128/JB.00969-09	journal	October 2009
YplA Is Exported by the Ysc, Ysa, and Flagellar Type III Secretion Systems of Yersinia enterocolitica Young, B. M.; Young, G. M. Journal of Bacteriology, Vol. 184, Issue 5 https://doi.org/10.1128/JB.184.5.1324-1334.2002	journal	March 2002
Secretion of Virulence Proteins from Campylobacter jejuni Is Dependent on a Functional Flagellar Export Apparatus Konkel, M. E.; Klena, J. D.; Rivera-Amill, V. Journal of Bacteriology, Vol. 186, Issue 11 https://doi.org/10.1128/JB.186.11.3296-3303.2004	journal	May 2004
IQGAP1 Is a Scaffold for Mitogen-Activated Protein Kinase Signaling Roy, Monideepa; Li, Zhigang; Sacks, David B. Molecular and Cellular Biology, Vol. 25, Issue 18 https://doi.org/10.1128/MCB.25.18.7940-7952.2005	journal	September 2005
Manipulation of Rab GTPase Function by Intracellular Bacterial Pathogens Brumell, John H.; Scidmore, Marci A. Microbiology and Molecular Biology Reviews, Vol. 71, Issue 4 https://doi.org/10.1128/MMBR.00023-07	journal	December 2007
Process of Protein Transport by the Type III Secretion System Ghosh, P. Microbiology and Molecular Biology Reviews, Vol. 68, Issue 4 https://doi.org/10.1128/MMBR.68.4.771-795.2004	journal	December 2004
Campylobacter colitis: histological immunohistochemical and ultrastructural findings. van Spreeuwel, J. P.; Duursma, G. C.; Meijer, C. J. Gut, Vol. 26, Issue 9 https://doi.org/10.1136/gut.26.9.945	journal	September 1985
A role for Campylobacter jejuni-induced enteritis in inflammatory bowel disease? Kalischuk, Lisa D.; Buret, Andre G. American Journal of Physiology-Gastrointestinal and Liver Physiology, Vol. 298, Issue 1 https://doi.org/10.1152/ajpgi.00193.2009	journal	January 2010
Transcriptome landscape of the human placenta Kim, Jinsil; Zhao, Keyan; Jiang, Peng BMC Genomics, Vol. 13, Issue 1 https://doi.org/10.1186/1471-2164-13-115	journal	January 2012
Invasion of epithelial cells by Campylobacter jejuni is independent of caveolae Konkel, Michael E.; Samuelson, Derrick R.; Eucker, Tyson P. Cell Communication and Signaling, Vol. 11, Issue 1 https://doi.org/10.1186/1478-811X-11-100	journal	January 2013
The Campylobacter jejuni CiaD effector protein activates MAP kinase signaling pathways and is required for the development of disease Samuelson, Derrick R.; Eucker, Tyson P.; Bell, Julia A. Cell Communication and Signaling, Vol. 11, Issue 1 https://doi.org/10.1186/1478-811X-11-79	journal	January 2013
Serine phosphorylation of cortactin is required for maximal host cell invasion by Campylobacter jejuni Samuelson, Derrick R.; Konkel, Michael E. Cell Communication and Signaling, Vol. 11, Issue 1 https://doi.org/10.1186/1478-811X-11-82	journal	January 2013
The signaling pathway of Campylobacter jejuni-induced Cdc42 activation: Role of fibronectin, integrin beta1, tyrosine kinases and guanine exchange factor Vav2 Krause-Gruszczynska, Malgorzata; Boehm, Manja; Rohde, Manfred Cell Communication and Signaling, Vol. 9, Issue 1 https://doi.org/10.1186/1478-811X-9-32	journal	January 2011
The DAVID Gene Functional Classification Tool: a novel biological module-centric algorithm to functionally analyze large gene lists Huang, Da; Sherman, Brad T.; Tan, Qina Genome Biology, Vol. 8, Issue 9 https://doi.org/10.1186/gb-2007-8-9-r183	journal	January 2007
Rac1 is deactivated at integrin activation sites through an IQGAP1–filamin-A–RacGAP1 pathway Jacquemet, Guillaume; Morgan, Mark R.; Byron, Adam Journal of Cell Science, Vol. 126, Issue 18 https://doi.org/10.1242/jcs.121988	journal	September 2013
The Salmonella SPI2 Effector SseI Mediates Long-Term Systemic Infection by Modulating Host Cell Migration McLaughlin, Laura M.; Govoni, Gregory R.; Gerke, Christiane PLoS Pathogens, Vol. 5, Issue 11 https://doi.org/10.1371/journal.ppat.1000671	journal	November 2009
A Novel Mouse Model of Campylobacter jejuni Gastroenteritis Reveals Key Pro-inflammatory and Tissue Protective Roles for Toll-like Receptor Signaling during Infection Stahl, Martin; Ries, Jenna; Vermeulen, Jenny PLoS Pathogens, Vol. 10, Issue 7 https://doi.org/10.1371/journal.ppat.1004264	journal	July 2014
The Campylobacter jejuni CiaC virulence protein is secreted from the flagellum and delivered to the cytosol of host cells Neal-McKinney, Jason M.; Konkel, Michael E. Frontiers in Cellular and Infection Microbiology, Vol. 2 https://doi.org/10.3389/fcimb.2012.00031	journal	January 2012
Campylobacter jejuni Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells Negretti, Nicholas M.; Clair, Geremy; Talukdar, Prabhat K. Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.00755	journal	April 2019
Cortactin: A multifunctional regulator of cellular invasiveness Kirkbride, Kellye C.; Sung, Bong Hwan; Sinha, Seema Cell Adhesion & Migration, Vol. 5, Issue 2 https://doi.org/10.4161/cam.5.2.14773	journal	March 2011

Similar Records

Campylobacter jejuni Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells

Journal Article · Wed Apr 10 20:00:00 EDT 2019 · Frontiers in Microbiology · OSTI ID:1572934

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Bacteria-host cell interactions
Cell invasion
Effector
Flagella
IQGAP1
Pathogenesis
RacGAP1
Rho GTPases
Type three secretion system

The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

Citation Formats

References (79)

Similar Records

Related Subjects