Engineering Clostridium cellulovorans for highly selective n ‐butanol production from cellulose in consolidated bioprocessing

Bao, Teng; Hou, Wenjie; Wu, Xuefeng; Lu, Li; Zhang, Xian; Yang, Shang‐Tian

doi:10.1002/bit.27789

Engineering Clostridium cellulovorans for highly selective n ‐butanol production from cellulose in consolidated bioprocessing

Journal Article · Fri Apr 23 00:00:00 EDT 2021 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/bit.27789· OSTI ID:1780198

Bao, Teng ^[1]; Hou, Wenjie ^[2]; Wu, Xuefeng ^[3]; Lu, Li ^[1]; Zhang, Xian ^[4]; ^[1]

William G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USA
William G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USA, College of Life Sciences Northwest A&,F University Yangling Shanxi China
William G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USA, School of Food and Biological Engineering Hefei University of Technology Hefei China
William G. Lowrie Department of Chemical and Biomolecular Engineering The Ohio State University Columbus Ohio USA, School of Biotechnology Jiangnan University Wuxi China

Abstract

Cellulosic n ‐butanol from renewable lignocellulosic biomass has gained increased interest. Previously, we have engineered Clostridium cellulovorans , a cellulolytic acidogen, to overexpress the bifunctional butyraldehyde/butanol dehydrogenase gene adh E2 from C. acetobutylicum for n ‐butanol production from crystalline cellulose. However, butanol production by this engineered strain had a relatively low yield of approximately 0.22 g/g cellulose due to the coproduction of ethanol and acids. We hypothesized that strengthening the carbon flux through the central butyryl‐CoA biosynthesis pathway and increasing intracellular NADH availability in C. cellulovorans adh E2 would enhance n ‐butanol production. In this study, thiolase ( thl A ^CA ) from C. acetobutylicum and 3‐hydroxybutyryl‐CoA dehydrogenase ( hbd ^CT ) from C. tyrobutyricum were overexpressed in C. cellulovorans adh E2 to increase the flux from acetyl‐CoA to butyryl‐CoA. In addition, ferredoxin‐NAD(P) ⁺ oxidoreductase ( fnr ), which can regenerate the intracellular NAD(P)H and thus increase butanol biosynthesis, was also overexpressed. Metabolic flux analyses showed that mutants overexpressing these genes had a significantly increased carbon flux toward butyryl‐CoA, which resulted in increased production of butyrate and butanol. The addition of methyl viologen as an electron carrier in batch fermentation further directed more carbon flux towards n ‐butanol biosynthesis due to increased reducing equivalent or NADH. The engineered strain C. cellulovorans adh E2‐ fnr ^CA ‐ thl A ^CA ‐ hbd ^CT produced n ‐butanol from cellulose at a 50% higher yield (0.34 g/g), the highest ever obtained in batch fermentation by any known bacterial strain. The engineered C. cellulovorans is thus a promising host for n ‐butanol production from cellulosic biomass in consolidated bioprocessing.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

Grant/Contract Number:: EE0007005

OSTI ID:: 1780198

Journal Information:: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 7 Vol. 118; ISSN 0006-3592

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United States

Language:: English

References (72)

Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations Sillers, Ryan; Al-Hinai, Mohab Ali; Papoutsakis, Eleftherios T. Biotechnology and Bioengineering, Vol. 102, Issue 1, p. 38-49 https://doi.org/10.1002/bit.22058	journal	January 2009
Thiolase engineering for enhanced butanol production in Clostridium acetobutylicum Mann, Miriam S.; Lütke-Eversloh, Tina Biotechnology and Bioengineering, Vol. 110, Issue 3 https://doi.org/10.1002/bit.24758	journal	November 2012
Metabolic process engineering of Clostridium tyrobutyricum Δ ack - adhE2 for enhanced n-butanol production from glucose: Effects of methyl viologen on NADH availability, flux distribution, and fermentation kinetics : Metabolic Process Engineering of Du, Yinming; Jiang, Wenyan; Yu, Mingrui Biotechnology and Bioengineering, Vol. 112, Issue 4 https://doi.org/10.1002/bit.25489	journal	December 2014
Metabolic engineering of Clostridium tyrobutyricum for n-butanol production through co-utilization of glucose and xylose : Metabolic Engineering of Yu, Le; Xu, Mengmeng; Tang, I-Ching Biotechnology and Bioengineering, Vol. 112, Issue 10 https://doi.org/10.1002/bit.25613	journal	June 2015
A novel in situ gas stripping-pervaporation process integrated with acetone-butanol-ethanol fermentation for hyper n-butanol production : Butanol production by an efficient recovery strategy Xue, Chuang; Liu, Fangfang; Xu, Mengmeng Biotechnology and Bioengineering, Vol. 113, Issue 1 https://doi.org/10.1002/bit.25666	journal	July 2015
Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum Fu, Hongxin; Lin, Meng; Tang, I‐Ching Biotechnology and Bioengineering, Vol. 118, Issue 2 https://doi.org/10.1002/bit.27602	journal	November 2020
Enzymatic investigations on butanol dehydrogenase and butyraldehyde dehydrogenase in extracts of Clostridium acetobutylicum D�rre, Peter; Kuhn, Anita; Gottwald, Matthias Applied Microbiology and Biotechnology, Vol. 26, Issue 3 https://doi.org/10.1007/BF00286322	journal	June 1987
Engineering Clostridium acetobutylicum with a histidine kinase knockout for enhanced n-butanol tolerance and production Xu, Mengmeng; Zhao, Jingbo; Yu, Le Applied Microbiology and Biotechnology, Vol. 99, Issue 2 https://doi.org/10.1007/s00253-014-6249-7	journal	December 2014
Metabolic engineering of Clostridium tyrobutyricum for n-butanol production: effects of CoA transferase Yu, Le; Zhao, Jingbo; Xu, Mengmeng Applied Microbiology and Biotechnology, Vol. 99, Issue 11 https://doi.org/10.1007/s00253-015-6566-5	journal	April 2015
Restriction modification system analysis and development of in vivo methylation for the transformation of Clostridium cellulovorans Yang, Xiaorui; Xu, Mengmeng; Yang, Shang-Tian Applied Microbiology and Biotechnology, Vol. 100, Issue 5 https://doi.org/10.1007/s00253-015-7141-9	journal	November 2015
Development of a shuttle plasmid without host restriction sites for efficient transformation and heterologous gene expression in Clostridium cellulovorans Bao, Teng; Zhao, Jingbo; Zhang, Qianxia Applied Microbiology and Biotechnology, Vol. 103, Issue 13 https://doi.org/10.1007/s00253-019-09882-0	journal	May 2019
Engineering Clostridium for improved solvent production: recent progress and perspective Cheng, Chi; Bao, Teng; Yang, Shang-Tian Applied Microbiology and Biotechnology, Vol. 103, Issue 14 https://doi.org/10.1007/s00253-019-09916-7	journal	May 2019
Improvement of butanol production in Clostridium acetobutylicum through enhancement of NAD(P)H availability Qi, Feng; Thakker, Chandresh; Zhu, Fayin Journal of Industrial Microbiology & Biotechnology, Vol. 45, Issue 11 https://doi.org/10.1007/s10295-018-2068-7	journal	August 2018
Cloning, Expression and Characterization of a Thiolase Gene from Clostridium pasteurianum Meng, Yonghong; Li, Jilun Biotechnology Letters, Vol. 28, Issue 16 https://doi.org/10.1007/s10529-006-9089-4	journal	June 2006
Recent advances in n-butanol and butyrate production using engineered Clostridium tyrobutyricum Bao, Teng; Feng, Jun; Jiang, Wenyan World Journal of Microbiology and Biotechnology, Vol. 36, Issue 9 https://doi.org/10.1007/s11274-020-02914-2	journal	August 2020
Comparative economic assessment of ABE fermentation based on cellulosic and non-cellulosic feedstocks Kumar, Manish; Goyal, Yogesh; Sarkar, Abhijit Applied Energy, Vol. 93 https://doi.org/10.1016/j.apenergy.2011.12.079	journal	May 2012
Butanol production from wood pulping hydrolysate in an integrated fermentation–gas stripping process Lu, Congcong; Dong, Jie; Yang, Shang-Tian Bioresource Technology, Vol. 143 https://doi.org/10.1016/j.biortech.2013.06.012	journal	September 2013
Enhancement of solvent production by overexpressing key genes of the acetone-butanol-ethanol fermentation pathway in Clostridium saccharoperbutylacetonicum N1-4 Wang, Shaohua; Dong, Sheng; Wang, Yi Bioresource Technology, Vol. 245 https://doi.org/10.1016/j.biortech.2017.09.024	journal	December 2017
Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose Cheng, Chi; Li, Weiming; Lin, Meng Bioresource Technology, Vol. 284 https://doi.org/10.1016/j.biortech.2019.03.145	journal	July 2019
n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases Bao, Teng; Zhao, Jingbo; Li, Jing Bioresource Technology, Vol. 285 https://doi.org/10.1016/j.biortech.2019.121316	journal	August 2019
n-Butanol production from lignocellulosic biomass hydrolysates without detoxification by Clostridium tyrobutyricum Δack-adhE2 in a fibrous-bed bioreactor Li, Jing; Du, Yinming; Bao, Teng Bioresource Technology, Vol. 289 https://doi.org/10.1016/j.biortech.2019.121749	journal	October 2019
Production of n-butanol from cassava bagasse hydrolysate by engineered Clostridium tyrobutyricum overexpressing adhE2: Kinetics and cost analysis Huang, Jin; Du, Yinming; Bao, Teng Bioresource Technology, Vol. 292 https://doi.org/10.1016/j.biortech.2019.121969	journal	November 2019
Co-valorization of paper mill sludge and corn steep liquor for enhanced n-butanol production with Clostridium tyrobutyricum Δcat1::adhE2 Cao, Xianshuang; Chen, Zhu; Liang, Liyan Bioresource Technology, Vol. 296 https://doi.org/10.1016/j.biortech.2019.122347	journal	January 2020
An application programming interface for CellNetAnalyzer Klamt, Steffen; von Kamp, Axel Biosystems, Vol. 105, Issue 2 https://doi.org/10.1016/j.biosystems.2011.02.002	journal	August 2011
Recent advances and state-of-the-art strategies in strain and process engineering for biobutanol production by Clostridium acetobutylicum Xue, Chuang; Zhao, Jingbo; Chen, Lijie Biotechnology Advances, Vol. 35, Issue 2 https://doi.org/10.1016/j.biotechadv.2017.01.007	journal	March 2017
Engineering clostridia for butanol production from biorenewable resources: from cells to process integration Wang, Jufang; Yang, Xiaorui; Chen, Chih-Chin Current Opinion in Chemical Engineering, Vol. 6 https://doi.org/10.1016/j.coche.2014.09.003	journal	November 2014
Recent progress in consolidated bioprocessing Olson, Daniel G.; McBride, John E.; Joe Shaw, A. Current Opinion in Biotechnology, Vol. 23, Issue 3, p. 396-405 https://doi.org/10.1016/j.copbio.2011.11.026	journal	June 2012
Decreased hydrogen production leads to selective butanol production in co-cultures of Clostridium thermocellum and Clostridium saccharoperbutylacetonicum strain N1-4 Nakayama, Shunichi; Bando, Yukiko; Ohnishi, Akihiro Journal of Bioscience and Bioengineering, Vol. 115, Issue 2 https://doi.org/10.1016/j.jbiosc.2012.08.020	journal	February 2013
Comparative proteomics analysis of high n-butanol producing metabolically engineered Clostridium tyrobutyricum Ma, Chao; Kojima, Kyoko; Xu, Ningning Journal of Biotechnology, Vol. 193 https://doi.org/10.1016/j.jbiotec.2014.10.036	journal	January 2015
Characterization of a highly thermostable ß-hydroxybutyryl CoA dehydrogenase from Clostridium acetobutylicum ATCC 824 Sommer, Bettina; Garbe, Daniel; Schrepfer, Patrick Journal of Molecular Catalysis B: Enzymatic, Vol. 98 https://doi.org/10.1016/j.molcatb.2013.10.014	journal	December 2013
Global transcriptional changes of Clostridium acetobutylicum cultures with increased butanol:acetone ratios Hönicke, Daniel; Janssen, Holger; Grimmler, Christina New Biotechnology, Vol. 29, Issue 4 https://doi.org/10.1016/j.nbt.2012.01.001	journal	May 2012
A novel strategy for sequential co-culture of Clostridium thermocellum and Clostridium beijerinckii to produce solvents from alkali extracted corn cobs Wen, Zhiqiang; Wu, Mianbin; Lin, Yijun Process Biochemistry, Vol. 49, Issue 11 https://doi.org/10.1016/j.procbio.2014.07.009	journal	November 2014
Process engineering of cellulosic n-butanol production from corn-based biomass using Clostridium cellulovorans Ou, Jianfa; Xu, Ningning; Ernst, Patrick Process Biochemistry, Vol. 62 https://doi.org/10.1016/j.procbio.2017.07.009	journal	November 2017
Intracellular metabolism analysis of Clostridium cellulovorans via modeling integrating proteomics, metabolomics and fermentation Ou, Jianfa; Bao, Teng; Ernst, Patrick Process Biochemistry, Vol. 89 https://doi.org/10.1016/j.procbio.2019.10.032	journal	February 2020
Enhanced direct fermentation from food waste to butanol and hydrogen by an amylolytic Clostridium Zhang, Chen; Li, Tinggang; Su, Guandong Renewable Energy, Vol. 153 https://doi.org/10.1016/j.renene.2020.01.151	journal	June 2020
Biobutanol Production from Crystalline Cellulose through Consolidated Bioprocessing Xin, Fengxue; Dong, Weiliang; Zhang, Wenming Trends in Biotechnology, Vol. 37, Issue 2 https://doi.org/10.1016/j.tibtech.2018.08.007	journal	February 2019
Designing Synthetic Microbial Consortia for Biofuel Production Jiang, Yujia; Dong, Weiliang; Xin, Fengxue Trends in Biotechnology, Vol. 38, Issue 8 https://doi.org/10.1016/j.tibtech.2020.02.002	journal	August 2020
Consolidated bioprocessing of cellulose to isobutanol using Clostridium thermocellum Lin, Paul P.; Mi, Luo; Morioka, Amy H. Metabolic Engineering, Vol. 31 https://doi.org/10.1016/j.ymben.2015.07.001	journal	September 2015
Metabolic and process engineering of Clostridium cellulovorans for biofuel production from cellulose Yang, Xiaorui; Xu, Mengmeng; Yang, Shang-Tian Metabolic Engineering, Vol. 32 https://doi.org/10.1016/j.ymben.2015.09.001	journal	November 2015
Enhanced solvent production by metabolic engineering of a twin-clostridial consortium Wen, Zhiqiang; Minton, Nigel P.; Zhang, Ying Metabolic Engineering, Vol. 39 https://doi.org/10.1016/j.ymben.2016.10.013	journal	January 2017
Engineering electron metabolism to increase ethanol production in Clostridium thermocellum Lo, Jonathan; Olson, Daniel G.; Murphy, Sean Jean-Loup Metabolic Engineering, Vol. 39 https://doi.org/10.1016/j.ymben.2016.10.018	journal	January 2017
Characterization of the Clostridium thermocellum AdhE, NfnAB, ferredoxin and Pfor proteins for their ability to support high titer ethanol production in Thermoanaerobacterium saccharolyticum Cui, Jingxuan; Olson, Daniel G.; Lynd, Lee R. Metabolic Engineering, Vol. 51 https://doi.org/10.1016/j.ymben.2018.09.006	journal	January 2019
High-Performance n -Butanol Recovery from Aqueous Solution by Pervaporation with a PDMS Mixed Matrix Membrane Filled with Zeolite Cheng, Chi; Liu, Fangfang; Yang, Hopen K. Industrial & Engineering Chemistry Research, Vol. 59, Issue 16 https://doi.org/10.1021/acs.iecr.9b06104	journal	March 2020
Metabolic Engineering of Clostridium cellulovorans to Improve Butanol Production by Consolidated Bioprocessing Wen, Zhiqiang; Ledesma-Amaro, Rodrigo; Lu, Minrui ACS Synthetic Biology, Vol. 9, Issue 2 https://doi.org/10.1021/acssynbio.9b00331	journal	December 2019
Opportunities and challenges for a sustainable energy future Chu, Steven; Majumdar, Arun Nature, Vol. 488, Issue 7411, p. 294-303 https://doi.org/10.1038/nature11475	journal	August 2012
How biotech can transform biofuels Lynd, Lee R.; Laser, Mark S.; Bransby, David Nature Biotechnology, Vol. 26, Issue 2, p. 169-172 https://doi.org/10.1038/nbt0208-169	journal	February 2008
Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum Kim, Sangwoo; Jang, Yu-Sin; Ha, Sung-Chul Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms9410	journal	September 2015
Reviving the Weizmann process for commercial n-butanol production Nguyen, Ngoc-Phuong-Thao; Raynaud, Céline; Meynial-Salles, Isabelle Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-05661-z	journal	September 2018
Comparative genomics of the mesophilic cellulosome‐producing Clostridium cellulovorans and its application to biofuel production via consolidated bioprocessing Tamaru, Yutaka; Miyake, Hideo; Kuroda, Kouichi Environmental Technology, Vol. 31, Issue 8-9 https://doi.org/10.1080/09593330.2010.490856	journal	July 2010
One hundred years of clostridial butanol fermentation Moon, Hyeon Gi; Jang, Yu-Sin; Cho, Changhee FEMS Microbiology Letters https://doi.org/10.1093/femsle/fnw001	journal	January 2016
Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum: Sporulation initiation in clostridia Steiner, Elisabeth; Dago, Angel E.; Young, Danielle I. Molecular Microbiology, Vol. 80, Issue 3 https://doi.org/10.1111/j.1365-2958.2011.07608.x	journal	March 2011
Purification and Properties of NADP-Dependent l(+)-3-Hydroxybutyryl-CoA Dehydrogenase from Clostridium kluyveri Madan, Vijay K.; Hillmer, Peter; Gottschalk, Gerhard European Journal of Biochemistry, Vol. 32, Issue 1 https://doi.org/10.1111/j.1432-1033.1973.tb02577.x	journal	January 1973
Unique genetic cassettes in a Thermoanaerobacterium contribute to simultaneous conversion of cellulose and monosugars into butanol Li, Tinggang; Zhang, Chen; Yang, Kun-Lin Science Advances, Vol. 4, Issue 3 https://doi.org/10.1126/sciadv.1701475	journal	March 2018
Butanol Production from Crystalline Cellulose by Cocultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4 Nakayama, Shunichi; Kiyoshi, Keiji; Kadokura, Toshimori Applied and Environmental Microbiology, Vol. 77, Issue 18 https://doi.org/10.1128/AEM.00706-11	journal	July 2011
Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose Higashide, Wendy; Li, Yongchao; Yang, Yunfeng Applied and Environmental Microbiology, Vol. 78, Issue 19 https://doi.org/10.1128/AEM.02335-12	journal	September 2012
Improved n -Butanol Production from Clostridium cellulovorans by Integrated Metabolic and Evolutionary Engineering Wen, Zhiqiang; Ledesma-Amaro, Rodrigo; Lin, Jianping Applied and Environmental Microbiology, Vol. 85, Issue 7 https://doi.org/10.1128/AEM.02560-18	journal	January 2019
The Issue of Secretion in Heterologous Expression of Clostridium cellulolyticum Cellulase-Encoding Genes in Clostridium acetobutylicum ATCC 824 Mingardon, Florence; Chanal, Angélique; Tardif, Chantal Applied and Environmental Microbiology, Vol. 77, Issue 9 https://doi.org/10.1128/AEM.03012-10	journal	March 2011
Isolation and Characterization of an Anaerobic, Cellulolytic Bacterium, Clostridium cellulovorans sp. nov Sleat, Robert; Mah, Robert A.; Robinson, Ralph Applied and Environmental Microbiology, Vol. 48, Issue 1 https://doi.org/10.1128/AEM.48.1.88-93.1984	journal	January 1984
Purification and properties of 3-hydroxybutyryl-coenzyme A dehydrogenase from Clostridium beijerinckii ("Clostridium butylicum") NRRL B593. Colby, G. D.; Chen, J. S. Applied and Environmental Microbiology, Vol. 58, Issue 10 https://doi.org/10.1128/AEM.58.10.3297-3302.1992	journal	January 1992
Heterologous Production, Assembly, and Secretion of a Minicellulosome by Clostridium acetobutylicum ATCC 824 Mingardon, F.; Perret, S.; Belaich, A. Applied and Environmental Microbiology, Vol. 71, Issue 3 https://doi.org/10.1128/AEM.71.3.1215-1222.2005	journal	March 2005
NADP+ Reduction with Reduced Ferredoxin and NADP+ Reduction with NADH Are Coupled via an Electron-Bifurcating Enzyme Complex in Clostridium kluyveri Wang, S.; Huang, H.; Moll, J. Journal of Bacteriology, Vol. 192, Issue 19 https://doi.org/10.1128/JB.00612-10	journal	July 2010
Genome Sequence of the Cellulosome-Producing Mesophilic Organism Clostridium cellulovorans 743B Tamaru, Y.; Miyake, H.; Kuroda, K. Journal of Bacteriology, Vol. 192, Issue 3 https://doi.org/10.1128/JB.01450-09	journal	November 2009
Production of Heterologous and Chimeric Scaffoldins by Clostridium acetobutylicum ATCC 824 Perret, S.; Casalot, L.; Fierobe, H. -P. Journal of Bacteriology, Vol. 186, Issue 1 https://doi.org/10.1128/JB.186.1.253-257.2004	journal	December 2003
Engineering Clostridial Aldehyde/Alcohol Dehydrogenase for Selective Butanol Production Cho, Changhee; Hong, Seungpyo; Moon, Hyeon Gi mBio, Vol. 10, Issue 1 https://doi.org/10.1128/mBio.02683-18	journal	January 2019
Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol Steen, Eric J.; Chan, Rossana; Prasad, Nilu Microbial Cell Factories, Vol. 7, Issue 1, Article No. 36 https://doi.org/10.1186/1475-2859-7-36	journal	January 2008
Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824 Kovács, Katalin; Willson, Benjamin J.; Schwarz, Katrin Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-117	journal	January 2013
Artificial symbiosis for acetone-butanol-ethanol (ABE) fermentation from alkali extracted deshelled corn cobs by co-culture of Clostridium beijerinckii and Clostridium cellulovorans Wen, Zhiqiang; Wu, Mianbin; Lin, Yijun Microbial Cell Factories, Vol. 13, Issue 1 https://doi.org/10.1186/s12934-014-0092-5	journal	July 2014
Metabolic engineering of Clostridium cellulolyticum for the production of n-butanol from crystalline cellulose Gaida, Stefan Marcus; Liedtke, Andrea; Jentges, Andreas Heinz Wilhelm Microbial Cell Factories, Vol. 15, Issue 1 https://doi.org/10.1186/s12934-015-0406-2	journal	January 2016
Developing a mesophilic co-culture for direct conversion of cellulose to butanol in consolidated bioprocess Wang, Zhenyu; Cao, Guangli; Zheng, Ju Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0266-3	journal	June 2015
Consolidated bioprocessing of butanol production from xylan by a thermophilic and butanologenic Thermoanaerobacterium sp. M5 Jiang, Yujia; Guo, Dong; Lu, Jiasheng Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1092-1	journal	April 2018
Metabolic engineering of Clostridium thermocellum for n-butanol production from cellulose Tian, Liang; Conway, Peter M.; Cervenka, Nicholas D. Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1524-6	journal	July 2019
Rebalancing Redox to Improve Biobutanol Production by Clostridium tyrobutyricum Ma, Chao; Ou, Jianfa; Xu, Ningning Bioengineering, Vol. 3, Issue 1 https://doi.org/10.3390/bioengineering3010002	journal	December 2015

Similar Records

n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases

Journal Article · Tue Apr 02 20:00:00 EDT 2019 · Bioresource Technology · OSTI ID:1572179

Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes

Journal Article · Thu Aug 06 20:00:00 EDT 2015 · Applied and Environmental Microbiology · OSTI ID:1470144

Engineering Clostridium cellulovorans for highly selective n ‐butanol production from cellulose in consolidated bioprocessing

Citation Formats

References (72)

Similar Records

Related Subjects