Double-stranded viral RNA persists in vitro and in vivo during prolonged infection of porcine reproductive and respiratory syndrome virus

Guo, Rui; Shang, Pengcheng; Carrillo, Celena A.; Sun, Zhi; Lakshmanappa, Yashavanth Shaan; Yan, Xingyu; Renukaradhya, Gourapura J.; McGill, Jodi; Jaing, Crystal J.; Niederwerder, Megan C.; Rowland, Raymond R.R.; Fang, Ying

doi:10.1016/j.virol.2018.08.006

Double-stranded viral RNA persists in vitro and in vivo during prolonged infection of porcine reproductive and respiratory syndrome virus

Journal Article · Mon Aug 27 00:00:00 EDT 2018 · Virology

DOI:https://doi.org/10.1016/j.virol.2018.08.006· OSTI ID:1779580

Guo, Rui ^[1]; Shang, Pengcheng ^[1]; Carrillo, Celena A. ^[2]; Sun, Zhi ^[3]; Lakshmanappa, Yashavanth Shaan ^[4]; Yan, Xingyu ^[1]; Renukaradhya, Gourapura J. ^[4]; McGill, Jodi ^[5]; Jaing, Crystal J. ^[2]; Niederwerder, Megan C. ^[1]; Rowland, Raymond R.R. ^[1]; Fang, Ying ^[6]

Kansas State Univ., Manhattan, KS (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
South Dakota State Univ., Brookings, SD (United States); Boehringer Ingelheim Asian Veterinary Research and Development Center, Shanghai (China)
The Ohio State Univ., Wooster, OH (United States). Food Animal Health Research Program (FAHRP), OARDC
Kansas State Univ., Manhattan, KS (United States); Iowa State Univ., Ames, IA (United States)
Kansas State Univ., Manhattan, KS (United States); South Dakota State Univ., Brookings, SD (United States)

In order to study the mechanism of PRRSV persistence, an in vitro model of persistence was developed by serially passaging PRRSV-infected MARC-145 cells 109 times. Viral persistence was detected to be associated with increased double-stranded (dsRNA) in the infected cells. In PRRSV infected pigs, reduced ratio of plus to minus strands of viral RNA was observed in lymphoid tissues from PRRSV persistent pigs at 52 days post infection. Viral dsRNA was mostly detected in the germinal center during persistent infection compared to the localization of dsRNA in the inter-follicular zones during acute infection. RNA array analysis of antiviral cytokines in persistently infected lymph nodes showed that the presence of dsRNA did not stimulate antiviral immunity. These results suggest that PRRSV dsRNA functions as a mediator for viral persistence. The localization of PRRSV dsRNA in the germinal center of lymphoid tissues reveals a novel mechanism for PRRSV persistence.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDA; USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1779580

Alternate ID(s):: OSTI ID: 23094988

Report Number(s):: LLNL-JRNL--820790; 1032457

Journal Information:: Virology, Journal Name: Virology Vol. 524; ISSN 0042-6822

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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