Development of a thermophilic coculture for corn fiber conversion to ethanol

Beri, Dhananjay; York, William S.; Lynd, Lee R.; Peña, Maria J.; Herring, Christopher D.

doi:10.1038/s41467-020-15704-z

Development of a thermophilic coculture for corn fiber conversion to ethanol

Journal Article · Wed Apr 22 00:00:00 EDT 2020 · Nature Communications

DOI:https://doi.org/10.1038/s41467-020-15704-z· OSTI ID:1779515

; ; Lynd, Lee R.; ; Herring, Christopher D.

Abstract

The fiber in corn kernels, currently unutilized in the corn to ethanol process, represents an opportunity for introduction of cellulose conversion technology. We report here that Clostridium thermocellum can solubilize over 90% of the carbohydrate in autoclaved corn fiber, including its hemicellulose component glucuronoarabinoxylan (GAX). However, Thermoanaerobacterium thermosaccharolyticum or several other described hemicellulose-fermenting thermophilic bacteria can only partially utilize this GAX. We describe the isolation of a previously undescribed organism, Herbinix spp . strain LL1355, from a thermophilic microbiome that can consume 85% of the recalcitrant GAX. We sequence its genome, and based on structural analysis of the GAX, identify six enzymes that hydrolyze GAX linkages. Combinations of up to four enzymes are successfully expressed in T. thermosaccharolyticum . Supplementation with these enzymes allows T. thermosaccharolyticum to consume 78% of the GAX compared to 53% by the parent strain and increases ethanol yield from corn fiber by 24%.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231; AC05-00OR22725

OSTI ID:: 1779515

Alternate ID(s):: OSTI ID: 1629027

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 11; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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