Nutrient Exposure Alters Microbial Composition, Structure, and Mercury Methylating Activity in Periphyton in a Contaminated Watershed

Carrell, Alyssa A.; Schwartz, Grace E.; Cregger, Melissa; Gionfriddo, Caitlin; Elias, Dwayne A.; Wilpiszeski, Regina; Klingeman, Dawn Marie; Wymore, Ann; Muller, Katherine A.; Brooks, Scott C.

doi:10.3389/fmicb.2021.647861

Title: Nutrient Exposure Alters Microbial Composition, Structure, and Mercury Methylating Activity in Periphyton in a Contaminated Watershed

Journal Article · Fri Mar 19 00:00:00 EDT 2021 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2021.647861· OSTI ID:1777727

Carrell, Alyssa A. ^[1]; Schwartz, Grace E. ^[2];

^[1];

^[3];

^[1]; Wilpiszeski, Regina ^[1];

^[1]; Wymore, Ann ^[1];

^[4];

^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Wofford College, Spartanburg, SC (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Smithsonian Environmental Research Center, Edgewater, MD (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

The conversion of mercury (Hg) to monomethylmercury (MMHg) is a critical area of concern in global Hg cycling. Periphyton biofilms may harbor significant amounts of MMHg but little is known about the Hg-methylating potential of the periphyton microbiome. Therefore, we used high-throughput amplicon sequencing of the 16S rRNA gene, ITS2 region, and Hg methylation gene pair (hgcAB) to characterize the archaea/bacteria, fungi, and Hg-methylating microorganisms in periphyton communities grown in a contaminated watershed in East Tennessee (United States). Furthermore, we examined how nutrient amendments (nitrate and/or phosphate) altered periphyton community structure and function. We found that bacterial/archaeal richness in experimental conditions decreased in summer and increased in autumn relative to control treatments, while fungal diversity generally increased in summer and decreased in autumn relative to control treatments. Interestingly, the Hg-methylating communities were dominated by Proteobacteria followed by Candidatus Atribacteria across both seasons. Surprisingly, Hg methylation potential correlated with numerous bacterial families that do not contain hgcAB, suggesting that the overall microbiome structure of periphyton communities influences rates of Hg transformation within these microbial mats. To further explore these complex community interactions, we performed a microbial network analysis and found that the nitrate-amended treatment resulted in the highest number of hub taxa that also corresponded with enhanced Hg methylation potential. This work provides insight into community interactions within the periphyton microbiome that may contribute to Hg cycling and will inform future research that will focus on establishing mixed microbial consortia to uncover mechanisms driving shifts in Hg cycling within periphyton habitats.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725; AC05-76RL01830

OSTI ID:: 1777727

Alternate ID(s):: OSTI ID: 1787306

Report Number(s):: PNNL-SA-158901

Journal Information:: Frontiers in Microbiology, Vol. 12, Issue 12; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

References (72)

Periphyton–water quality relationships in fertilized fishponds with artificial substrates Azim, M. E.; Milstein, A.; Wahab, M. A. Aquaculture, Vol. 228, Issue 1-4 https://doi.org/10.1016/S0044-8486(03)00319-3	journal	December 2003
Methanogens: Principal Methylators of Mercury in Lake Periphyton Hamelin, Stéphanie; Amyot, Marc; Barkay, Tamar Environmental Science & Technology, Vol. 45, Issue 18 https://doi.org/10.1021/es2010072	journal	September 2011
Development and Validation of Broad-Range Qualitative and Clade-Specific Quantitative Molecular Probes for Assessing Mercury Methylation in the Environment Christensen, Geoff A.; Wymore, Ann M.; King, Andrew J. Applied and Environmental Microbiology, Vol. 82, Issue 19 https://doi.org/10.1128/AEM.01271-16	journal	July 2016
Importance of sulfate reducing bacteria in mercury methylation and demethylation in periphyton from Bolivian Amazon region Achá, Darío; Hintelmann, Holger; Yee, Janet Chemosphere, Vol. 82, Issue 6 https://doi.org/10.1016/j.chemosphere.2010.10.050	journal	February 2011
Scoring Species for Synthetic Community Design: Network Analyses of Functional Core Microbiomes Toju, Hirokazu; Abe, Masato S.; Ishii, Chiharu Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.01361	journal	June 2020
Ecosystem Controls on Methylmercury Production by Periphyton Biofilms in a Contaminated Stream: Implications for Predictive Modeling Schwartz, Grace E.; Olsen, Todd A.; Muller, Katherine A. Environmental Toxicology and Chemistry, Vol. 38, Issue 11 https://doi.org/10.1002/etc.4551	journal	September 2019
Copper accumulation and toxicity in fluvial periphyton: The influence of exposure history Serra, A.; Corcoll, N.; Guasch, H. Chemosphere, Vol. 74, Issue 5 https://doi.org/10.1016/j.chemosphere.2008.10.036	journal	February 2009
Association of a Specific Algal Group with Methylmercury Accumulation in Periphyton of a Tropical High-Altitude Andean Lake Lanza, William G.; Achá, Darío; Point, David Archives of Environmental Contamination and Toxicology, Vol. 72, Issue 1 https://doi.org/10.1007/s00244-016-0324-2	journal	November 2016
Impacts of phosphorus concentration and light intensity on river periphyton biomass and community structure McCall, Stephanie J.; Hale, Michelle S.; Smith, James T. Hydrobiologia, Vol. 792, Issue 1 https://doi.org/10.1007/s10750-016-3067-1	journal	February 2017
Network hubs in root-associated fungal metacommunities Toju, Hirokazu; Tanabe, Akifumi S.; Sato, Hirotoshi Microbiome, Vol. 6, Issue 1 https://doi.org/10.1186/s40168-018-0497-1	journal	June 2018
Velocity and Sediment Disturbance of Periphyton in Headwater Streams: Biomass and Metabolism Smith, Robert A.; Duncan, Maurice J. Journal of the North American Benthological Society, Vol. 18, Issue 2 https://doi.org/10.2307/1468462	journal	June 1999
Mercury Methylation by Novel Microorganisms from New Environments Gilmour, Cynthia C.; Podar, Mircea; Bullock, Allyson L. Environmental Science & Technology, Vol. 47, Issue 20 https://doi.org/10.1021/es403075t	journal	September 2013
Mercury cycling in freshwater systems - An updated conceptual model Branfireun, Brian A.; Cosio, Claudia; Poulain, Alexandre J. Science of The Total Environment, Vol. 745 https://doi.org/10.1016/j.scitotenv.2020.140906	journal	November 2020
Trimmomatic: a flexible trimmer for Illumina sequence data Bolger, Anthony M.; Lohse, Marc; Usadel, Bjoern Bioinformatics, Vol. 30, Issue 15 https://doi.org/10.1093/bioinformatics/btu170	journal	April 2014
Seasonal variation in nutrient limitation of microbial biofilms colonizing organic and inorganic substrata in streams Hoellein, Timothy J.; Tank, Jennifer L.; Kelly, John J. Hydrobiologia, Vol. 649, Issue 1 https://doi.org/10.1007/s10750-010-0276-x	journal	May 2010
Interactions between atrazine and phosphorus in aquatic systems: Effects on phytoplankton and periphyton Baxter, Leilan R.; Sibley, Paul K.; Solomon, Keith R. Chemosphere, Vol. 90, Issue 3 https://doi.org/10.1016/j.chemosphere.2012.09.011	journal	January 2013
A novel method to assess effects of chemical stressors on natural biofilm structure and function Costello, David M.; Rosi-Marshall, Emma J.; Shaw, Lawton E. Freshwater Biology, Vol. 61, Issue 12 https://doi.org/10.1111/fwb.12641	journal	December 2015
Periphytic algae colonization driven by variable environmental components in a temperate floodplain lake Pfeiffer, Tanja Žuna; Mihaljević, Melita; Stević, Filip Annales de Limnologie - International Journal of Limnology, Vol. 49, Issue 3 https://doi.org/10.1051/limn/2013050	journal	January 2013
Diurnal Cycle of Oxygen and Sulfide Microgradients and Microbial Photosynthesis in a Cyanobacterial Mat Sediment Jørgensen, Bo B.; Revsbech, Niels P.; Blackburn, T. Henry Applied and Environmental Microbiology, Vol. 38, Issue 1 https://doi.org/10.1128/AEM.38.1.46-58.1979	journal	January 1979
Seasonal variations in response of periphytic algal community to nutrient enrichment in the river Ganga (Varanasi, India) Yadav, Arpana; Kumar, Dhananjay; Singh, Ram Sharan Annales de Limnologie - International Journal of Limnology, Vol. 54 https://doi.org/10.1051/limn/2018025	journal	January 2018
Proposing a resolution to debates on diversity partitioning Chao, Anne; Chiu, Chun-Huo; Hsieh, T. C. Ecology, Vol. 93, Issue 9 https://doi.org/10.1890/11-1817.1	journal	September 2012
Expanded Phylogenetic Diversity and Metabolic Flexibility of Mercury-Methylating Microorganisms McDaniel, Elizabeth A.; Peterson, Benjamin D.; Stevens, Sarah L. R. mSystems, Vol. 5, Issue 4 https://doi.org/10.1128/mSystems.00299-20	journal	August 2020
Seasonal changes in peryphytic microbial metabolism determining mercury methylation in a tropical wetland Lázaro, Wilkinson L.; Díez, Sergi; da Silva, Carolina J. Science of The Total Environment, Vol. 627 https://doi.org/10.1016/j.scitotenv.2018.01.186	journal	June 2018
Establishing nutrient criteria in streams Dodds, Walter K. K.; Welch, Eugene B. Journal of the North American Benthological Society, Vol. 19, Issue 1 https://doi.org/10.2307/1468291	journal	March 2000
Erythrolic acids A–E, Meroterpenoids from a Marine-Derived Erythrobacter sp. Hu, Youcai; Legako, Aaron G.; Espindola, Ana Paula D. M. The Journal of Organic Chemistry, Vol. 77, Issue 7 https://doi.org/10.1021/jo300197z	journal	March 2012
Short-term Effects of Elevated Velocity and Sediment Abrasion on Benthic Algal Communities Francoeur, Steven N.; Biggs, Barry J. F. Hydrobiologia, Vol. 561, Issue 1 https://doi.org/10.1007/s10750-005-1604-4	journal	May 2006
The Genetic Basis for Bacterial Mercury Methylation Parks, J. M.; Johs, A.; Podar, M. Science, Vol. 339, Issue 6125, p. 1332-1335 https://doi.org/10.1126/science.1230667	journal	February 2013
Global prevalence and distribution of genes and microorganisms involved in mercury methylation Podar, Mircea; Gilmour, Cynthia C.; Brandt, Craig C. Science Advances, Vol. 1, Issue 9 https://doi.org/10.1126/sciadv.1500675	journal	October 2015
Biotic formation of methylmercury: A bio–physico–chemical conundrum Bravo, Andrea G.; Cosio, Claudia Limnology and Oceanography, Vol. 65, Issue 5 https://doi.org/10.1002/lno.11366	journal	November 2019
Kinetics of Methylmercury Production Revisited Olsen, Todd A.; Muller, Katherine A.; Painter, Scott L. Environmental Science & Technology, Vol. 52, Issue 4 https://doi.org/10.1021/acs.est.7b05152	journal	January 2018
Nutrient and light limitation of periphyton in the River Thames: Implications for catchment management Bowes, M. J.; Ings, N. L.; McCall, S. J. Science of The Total Environment, Vol. 434 https://doi.org/10.1016/j.scitotenv.2011.09.082	journal	September 2012
History of mercury use and environmental contamination at the Oak Ridge Y-12 Plant Brooks, Scott C.; Southworth, George R. Environmental Pollution, Vol. 159, Issue 1 https://doi.org/10.1016/j.envpol.2010.09.009	journal	January 2011
Cyanobacteria as regulators of methylmercury production in periphyton Lázaro, Wilkinson L.; Díez, Sergi; Bravo, Andrea G. Science of The Total Environment, Vol. 668 https://doi.org/10.1016/j.scitotenv.2019.02.233	journal	June 2019
Syntrophic pathways for microbial mercury methylation Yu, Ri-Qing; Reinfelder, John R.; Hines, Mark E. The ISME Journal, Vol. 12, Issue 7 https://doi.org/10.1038/s41396-018-0106-0	journal	March 2018
Artifact formation of methyl mercury during aqueous distillation and alternative techniques for the extraction of methyl mercury from environmental samples Bloom, N. S.; Colman, John A.; Barber, Lee Fresenius' Journal of Analytical Chemistry, Vol. 358, Issue 3 https://doi.org/10.1007/s002160050432	journal	June 1997
Sediment Microbial Community Composition and Methylmercury Pollution at Four Mercury Mine?Impacted Sites Batten, K. M.; Scow, K. M. Microbial Ecology, Vol. 46, Issue 4 https://doi.org/10.1007/s00248-003-1005-z	journal	December 2003
Mercury methylation in periphyton of the Florida Everglades Cleckner, Lisa B.; Gilmour, Cynthia C.; Hurley, James P. Limnology and Oceanography, Vol. 44, Issue 7 https://doi.org/10.4319/lo.1999.44.7.1815	journal	October 1999
VSEARCH: a versatile open source tool for metagenomics Rognes, Torbjørn; Flouri, Tomáš; Nichols, Ben PeerJ, Vol. 4 https://doi.org/10.7717/peerj.2584	journal	January 2016
The potential of Cycloclasticus and Altererythrobacter strains for use in bioremediation of petroleum-aromatic-contaminated tropical marine environments Teramoto, Maki; Suzuki, Masahito; Hatmanti, Ariani Journal of Bioscience and Bioengineering, Vol. 110, Issue 1 https://doi.org/10.1016/j.jbiosc.2009.12.008	journal	July 2010
Contribution of Aerobic Photoheterotrophic Bacteria to the Carbon Cycle in the Ocean Kolber, Z. S. Science, Vol. 292, Issue 5526 https://doi.org/10.1126/science.1059707	journal	June 2001
Global mapping of freshwater nutrient enrichment and periphyton growth potential McDowell, R. W.; Noble, A.; Pletnyakov, P. Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-60279-w	journal	February 2020
Periphyton Biofilms Influence Net Methylmercury Production in an Industrially Contaminated System Olsen, Todd A.; Brandt, Craig C.; Brooks, Scott C. Environmental Science & Technology, Vol. 50, Issue 20 https://doi.org/10.1021/acs.est.6b01538	journal	September 2016
Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 Bolyen, Evan; Rideout, Jai Ram; Dillon, Matthew R. Nature Biotechnology, Vol. 37, Issue 8 https://doi.org/10.1038/s41587-019-0209-9	journal	July 2019
Effects of Cr III and Pb on the bioaccumulation and toxicity of Cd in tropical periphyton communities: Implications of pulsed metal exposures Bere, Taurai; Chia, Mathias Ahii; Tundisi, José Galizia Environmental Pollution, Vol. 163 https://doi.org/10.1016/j.envpol.2011.12.028	journal	April 2012
Molecular Characterization of Mercury Binding Ligands Released by Freshwater Algae Grown at Three Photoperiods Mangal, Vaughn; Phung, Ta; Nguyen, Thien Q. Frontiers in Environmental Science, Vol. 6 https://doi.org/10.3389/fenvs.2018.00155	journal	January 2019
Evidence for Regulation of Monomethyl Mercury by Nitrate in a Seasonally Stratified, Eutrophic Lake Todorova, Svetoslava G.; Driscoll, Charles T.; Matthews, David A. Environmental Science & Technology, Vol. 43, Issue 17 https://doi.org/10.1021/es900887b	journal	September 2009
Diverse Communities of hgcAB ⁺ Microorganisms Methylate Mercury in Freshwater Sediments Subjected to Experimental Sulfate Loading Jones, Daniel S.; Johnson, Nathan W.; Mitchell, Carl P. J. Environmental Science & Technology, Vol. 54, Issue 22 https://doi.org/10.1021/acs.est.0c02513	journal	November 2020
Carbon Amendments Alter Microbial Community Structure and Net Mercury Methylation Potential in Sediments Christensen, Geoff A.; Somenahally, Anil C.; Moberly, James G. Applied and Environmental Microbiology, Vol. 84, Issue 3 https://doi.org/10.1128/AEM.01049-17	journal	November 2017
Shifts in mercury methylation across a peatland chronosequence: From sulfate reduction to methanogenesis and syntrophy Hu, Haiyan; Wang, Baolin; Bravo, Andrea G. Journal of Hazardous Materials, Vol. 387 https://doi.org/10.1016/j.jhazmat.2019.121967	journal	April 2020
DADA2: High-resolution sample inference from Illumina amplicon data Callahan, Benjamin J.; McMurdie, Paul J.; Rosen, Michael J. Nature Methods, Vol. 13, Issue 7 https://doi.org/10.1038/nmeth.3869	journal	May 2016
The Populus holobiont: dissecting the effects of plant niches and genotype on the microbiome Cregger, M. A.; Veach, A. M.; Yang, Z. K. Microbiome, Vol. 6, Issue 1 https://doi.org/10.1186/s40168-018-0413-8	journal	February 2018
Cadmium speciation and accumulation in periphyton in a small stream with dynamic concentration variations Bradac, Philippe; Wagner, Bettina; Kistler, David Environmental Pollution, Vol. 158, Issue 3 https://doi.org/10.1016/j.envpol.2009.10.031	journal	March 2010
Influence of experimental, environmental, and geographic factors on nutrient-diffusing substrate experiments in running waters Beck, Whitney S.; Rugenski, Amanda T.; Poff, N. LeRoy Freshwater Biology, Vol. 62, Issue 10 https://doi.org/10.1111/fwb.12989	journal	August 2017
Communal metabolism by Methylococcaceae and Methylophilaceae is driving rapid aerobic methane oxidation in sediments of a shallow seep near Elba, Italy Taubert, Martin; Grob, Carolina; Crombie, Andrew Environmental Microbiology, Vol. 21, Issue 10 https://doi.org/10.1111/1462-2920.14728	journal	July 2019
Seasonal Changes in Chlorophyll a Response to Nutrient Amendments in a North Shore Tributary of Lake Superior Allen, Nan Sparr; Hershey, Anne E. Journal of the North American Benthological Society, Vol. 15, Issue 2 https://doi.org/10.2307/1467946	journal	June 1996
High-yield synthesis of milligram amounts of isotopically enriched methylmercury(CH3198HgCl) Bancon-Montigny, Chrystelle; Yang, Lu; Sturgeon, Ralph E. Applied Organometallic Chemistry, Vol. 18, Issue 2 https://doi.org/10.1002/aoc.572	journal	January 2004
Sulfate-Reducing Bacteria Methylate Mercury at Variable Rates in Pure Culture and in Marine Sediments King, J. K.; Kostka, J. E.; Frischer, M. E. Applied and Environmental Microbiology, Vol. 66, Issue 6, p. 2430-2437 https://doi.org/10.1128/AEM.66.6.2430-2437.2000	journal	June 2000
Microbial Hub Taxa Link Host and Abiotic Factors to Plant Microbiome Variation Agler, Matthew T.; Ruhe, Jonas; Kroll, Samuel PLOS Biology, Vol. 14, Issue 1 https://doi.org/10.1371/journal.pbio.1002352	journal	January 2016
Confounding factors in algal phosphorus limitation experiments Beck, Whitney S.; Hall, Ed K. PLOS ONE, Vol. 13, Issue 10 https://doi.org/10.1371/journal.pone.0205684	journal	October 2018
Periphyton as an important source of methylmercury in Everglades water and food web Xiang, Yuping; Liu, Guangliang; Yin, Yongguang Journal of Hazardous Materials, Vol. 410 https://doi.org/10.1016/j.jhazmat.2020.124551	journal	May 2021
Microelectrode studies of the photosynthesis and O ₂ , H ₂ S, and pH profiles of a microbial mat1: O ₂ , H ₂ S, and pH in microbial mat Revsbech, Niels Peter; Jorgensen, Bo Barker; Blackburn, T. Henry Limnology and Oceanography, Vol. 28, Issue 6 https://doi.org/10.4319/lo.1983.28.6.1062	journal	November 1983
Paludibacter propionicigenes gen. nov., sp. nov., a novel strictly anaerobic, Gram-negative, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil in Japan Ueki, A. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 56, Issue 1 https://doi.org/10.1099/ijs.0.63896-0	journal	January 2006
Isolation of a member of the candidate phylum ‘Atribacteria’ reveals a unique cell membrane structure Katayama, Taiki; Nobu, Masaru K.; Kusada, Hiroyuki Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-20149-5	journal	December 2020
A Hidden Pitfall in the Preparation of Agar Media Undermines Microorganism Cultivability Tanaka, Tomohiro; Kawasaki, Kosei; Daimon, Serina Applied and Environmental Microbiology, Vol. 80, Issue 24 https://doi.org/10.1128/AEM.02741-14	journal	October 2014
Diversity and Evenness: A Unifying Notation and Its Consequences Hill, M. O. Ecology, Vol. 54, Issue 2 https://doi.org/10.2307/1934352	journal	March 1973
Abundance of kinless hubs within soil microbial networks are associated with high functional potential in agricultural ecosystems Shi, Yu; Delgado-Baquerizo, Manuel; Li, Yuntao Environment International, Vol. 142 https://doi.org/10.1016/j.envint.2020.105869	journal	September 2020
Mechanisms of removing pollutants from aqueous solutions by microorganisms and their aggregates: A review Wu, Yonghong; Li, Tianling; Yang, Linzhang Bioresource Technology, Vol. 107 https://doi.org/10.1016/j.biortech.2011.12.088	journal	March 2012
Mercury Methylation Genes Identified across Diverse Anaerobic Microbial Guilds in a Eutrophic Sulfate-Enriched Lake Peterson, Benjamin D.; McDaniel, Elizabeth A.; Schmidt, Anna G. Environmental Science & Technology, Vol. 54, Issue 24 https://doi.org/10.1021/acs.est.0c05435	journal	November 2020
Interpretation of Shotgun Proteomic Data: The Protein Inference Problem Nesvizhskii, Alexey I.; Aebersold, Ruedi Molecular & Cellular Proteomics, Vol. 4, Issue 10 https://doi.org/10.1074/mcp.R500012-MCP200	journal	July 2005
Aerobic and Anaerobic Bacterial Mercury Uptake is Driven by Algal Organic Matter Composition and Molecular Weight Mangal, Vaughn; Stenzler, Benjamin R.; Poulain, Alexandre J. Environmental Science & Technology, Vol. 53, Issue 1 https://doi.org/10.1021/acs.est.8b04909	journal	November 2018
Algal and cyanobacterial secondary metabolites in freshwaters: a comparison of allelopathic compounds and toxins Leflaive, Jose´Phine; Ten-Hage, LoÏC Freshwater Biology, Vol. 52, Issue 2 https://doi.org/10.1111/j.1365-2427.2006.01689.x	journal	February 2007
Isolation and characterization of Erythrobacter sp. strains from the upper ocean Koblížek, Michal; Béjà, Oded; Bidigare, Robert R. Archives of Microbiology, Vol. 180, Issue 5 https://doi.org/10.1007/s00203-003-0596-6	journal	September 2003

Similar Records

Distribution of mercury-cycling genes in the Arctic and equatorial Pacific Oceans and their relationship to mercury speciation

Journal Article · Mon Aug 26 00:00:00 EDT 2019 · Limnology and Oceanography · OSTI ID:1777727

Bowman, Katlin; Collins, Eric; Agather, Alison; +6 more

Incorporating concentration-dependent sediment microbial activity into methylmercury production kinetics modeling

Journal Article · Fri Oct 29 00:00:00 EDT 2021 · Environmental Science: Processes & Impacts · OSTI ID:1777727

Schwartz, Grace E.; Muller, Katherine A.; Rathore, Saubhagya S.; +7 more

Ecosystem Controls on Methylmercury Production by Periphyton Biofilms in a Contaminated Stream: Implications for Predictive Modeling

Journal Article · Fri Nov 01 00:00:00 EDT 2019 · Environmental Toxicology and Chemistry · OSTI ID:1777727

Schwartz, Grace E.; Olsen, Todd A.; Muller, Katherine A.; +1 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
periphyton
mercury
microbiome
methylmercury
nutrient addition

Title: Nutrient Exposure Alters Microbial Composition, Structure, and Mercury Methylating Activity in Periphyton in a Contaminated Watershed

Citation Formats

References (72)

Similar Records

Related Subjects