Surface chemistry-mediated modulation of adsorbed albumin folding state specifies nanocarrier clearance by distinct macrophage subsets

Vincent, Michael P.; Bobbala, Sharan; Karabin, Nicholas B.; Frey, Molly; Liu, Yugang; Navidzadeh, Justin O.; Stack, Trevor; Scott, Evan A.

doi:10.1038/s41467-020-20886-7

Surface chemistry-mediated modulation of adsorbed albumin folding state specifies nanocarrier clearance by distinct macrophage subsets

Journal Article · Wed Jan 27 23:00:00 EST 2021 · Nature Communications

DOI:https://doi.org/10.1038/s41467-020-20886-7· OSTI ID:1777295

Vincent, Michael P. ^[1]; Bobbala, Sharan ^[1]; Karabin, Nicholas B. ^[1]; Frey, Molly ^[1]; ^[1]; Navidzadeh, Justin O. ^[1]; Stack, Trevor ^[1]; ^[1]

Northwestern Univ., Evanston, IL (United States)

Controlling nanocarrier interactions with the immune system requires a thorough understanding of the surface properties that modulate protein adsorption in biological fluids, since the resulting protein corona redefines cellular interactions with nanocarrier surfaces. Albumin is initially one of the dominant proteins to adsorb to nanocarrier surfaces, a process that is considered benign or beneficial by minimizing opsonization or inflammation. Here, we demonstrate the surface chemistry of a model nanocarrier can be engineered to stabilize or denature the three-dimensional conformation of adsorbed albumin, which respectively promotes evasion or non-specific clearance in vivo. Interestingly, certain common chemistries that have long been considered to convey stealth properties denature albumin to promote nanocarrier recognition by macrophage class A1 scavenger receptors, providing a means for their eventual removal from systemic circulation. We establish that the surface chemistry of nanocarriers can be specified to modulate adsorbed albumin structure and thereby tune clearance by macrophage scavenger receptors.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI ID:: 1777295

Alternate ID(s):: OSTI ID: 1779012

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 12; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Surface chemistry-mediated modulation of adsorbed albumin folding state specifies nanocarrier clearance by distinct macrophage subsets

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