Subacute safety assessment of recombinant Lactococcus lactis on the gut microbiota of male Sprague–Dawley rats

Chen, Guowei; Yang, Zixin; Cao, Chunting; Xiao, Xue; Huang, Yadong; Tian, Lingmin; Bai, Weibin

doi:10.1002/jsfa.11231

Subacute safety assessment of recombinant Lactococcus lactis on the gut microbiota of male Sprague–Dawley rats

Journal Article · Fri Apr 09 00:00:00 EDT 2021 · Journal of the Science of Food and Agriculture

DOI:https://doi.org/10.1002/jsfa.11231· OSTI ID:1775556

Chen, Guowei ^[1]; Yang, Zixin ^[1]; Cao, Chunting ^[1]; Xiao, Xue ^[2]; Huang, Yadong ^[2]; ^[1]; Bai, Weibin ^[1]

Department of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection Jinan University Guangzhou China
Department of Cell Biology, College of Life Science and Technology Jinan University Guangzhou China

Abstract BACKGROUND

Lactococcus lactis strain pGSMT/MG1363 is a genetically modified microorganism (GMM) that constitutively expresses human metallothionein‐I fusion protein to combine with intracellular lead. Unlike traditional probiotics, pGSMT/MG1363 lacks a history of safe use in food. Administration of microorganism could influence the gut microbial community and consequently confer health benefits or cause disadvantages to the host. To date, little has been done to assess the influence of recombinant strain pGSMT/MG1363 on the stability of gut microbiota.

RESULTS

Liver, testis and kidney sections of male Sprague–Dawley rats orally administered pGSMT/MG1363 for 6 weeks showed normal structure and no pathological damage. There were no adverse effects on the analyzed serum biochemical parameters between the pGSMT/MG1363 group and the MG1363 group. Principal coordinate analysis showed that, compared with the MG1363 group, the 6‐week‐old fecal gut microbiota of rats fed with pGSMT/MG1363 was not significantly different (Adonis, P = 0.802). pGSMT/MG1363 treatment for 6 weeks did not significantly change the relative abundance of gut microbiota at the phylum and genus levels in comparison with MG1363 treatment.

CONCLUSION

Compared to the non‐GM strain MG1363 group, administration of the recombinant strain pGSMT/MG1363 for 6 weeks showed no adverse effects on the analyzed physiological parameters and gut microbial compositions of male Sprague–Dawley rats. The results suggested that, in terms of gut microbiota stability, pGSMT/MG1363 could be considered as safe as MG1363, at least for short‐term intake. Further toxicological evaluations still need to be considered before drawing a definite conclusion concerning the safe use of pGSMT/MG1363. © 2021 Society of Chemical Industry.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

OSTI ID:: 1775556

Journal Information:: Journal of the Science of Food and Agriculture, Journal Name: Journal of the Science of Food and Agriculture Journal Issue: 14 Vol. 101; ISSN 0022-5142

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

References (30)

The effect of genetically modified Lactobacillus plantarum 590 on the gut health of sprague-dawley rats Liu, Hai-Yan; Xu, Wen-Tao; Yuan, Yan-Fang IUBMB Life, Vol. 64, Issue 7 https://doi.org/10.1002/iub.1027	journal	May 2012
The effects of RecO deficiency in Lactococcus lactis NZ9000 on resistance to multiple environmental stresses: Effects of RecO deficiency in L. lactis on resistance to environmental stresses Zhang, Mengru; Chen, Jian; Zhang, Juan Journal of the Science of Food and Agriculture, Vol. 94, Issue 15 https://doi.org/10.1002/jsfa.6662	journal	May 2014
Chromium(VI) bioremediation by probiotics: Chromium(VI) bioremediation by probiotics Younan, Soraia; Sakita, Gabriel Z.; Albuquerque, Talita R. Journal of the Science of Food and Agriculture, Vol. 96, Issue 12 https://doi.org/10.1002/jsfa.7725	journal	April 2016
Liver Function of Male Rats Exposed to Manganese at Different Time Points Zhu, Xiaonian; Yang, Lin; He, Yonghua Biological Trace Element Research, Vol. 198, Issue 1 https://doi.org/10.1007/s12011-020-02067-1	journal	February 2020
A Phase I Trial With Transgenic Bacteria Expressing Interleukin-10 in Crohn’s Disease Braat, Henri; Rottiers, Pieter; Hommes, Daniel W. Clinical Gastroenterology and Hepatology, Vol. 4, Issue 6 https://doi.org/10.1016/j.cgh.2006.03.028	journal	June 2006
Safety assessment of genetically modified milk containing human beta-defensin-3 on rats by a 90-day feeding study Chen, Xin; Gao, Ming-Qing; Liang, Dong Food and Chemical Toxicology, Vol. 100 https://doi.org/10.1016/j.fct.2016.12.012	journal	February 2017
Effects of cyanidin-3-O-glucoside on 3-chloro-1,2-propanediol induced intestinal microbiota dysbiosis in rats Chen, Guowei; Wang, Gang; Zhu, Cuijuan Food and Chemical Toxicology, Vol. 133 https://doi.org/10.1016/j.fct.2019.110767	journal	November 2019
Safety and risk assessment of the genetically modified Lactococci on rats intestinal bacterial flora Lee, Kai-Chien; Liu, Chin-Feng; Lin, Tzu-Hsing International Journal of Food Microbiology, Vol. 142, Issue 1-2 https://doi.org/10.1016/j.ijfoodmicro.2010.06.018	journal	August 2010
Hepatoprotective effect of total flavonoids of Mallotus apelta (Lour.) Muell.Arg. leaf against carbon tetrachloride-induced liver fibrosis in rats via modulation of TGF-β1/Smad and NF-κB signaling pathways Zhang, Bo; Lai, Ling; Tan, Yanjun Journal of Ethnopharmacology, Vol. 254 https://doi.org/10.1016/j.jep.2020.112714	journal	May 2020
Advances in metallothionein structure and functions Vašák, Milan Journal of Trace Elements in Medicine and Biology, Vol. 19, Issue 1 https://doi.org/10.1016/j.jtemb.2005.03.003	journal	September 2005
Gut–liver axis: The impact of gut microbiota on non alcoholic fatty liver disease Compare, D.; Coccoli, P.; Rocco, A. Nutrition, Metabolism and Cardiovascular Diseases, Vol. 22, Issue 6 https://doi.org/10.1016/j.numecd.2012.02.007	journal	June 2012
An insight into the complex roles of metallothioneins in malignant diseases with emphasis on (sub)isoforms/isoforms and epigenetics phenomena Krizkova, Sona; Kepinska, Marta; Emri, Gabriella Pharmacology & Therapeutics, Vol. 183 https://doi.org/10.1016/j.pharmthera.2017.10.004	journal	March 2018
Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration Wang, J.; Zhou, G.; Chen, C. Toxicology Letters, Vol. 168, Issue 2 https://doi.org/10.1016/j.toxlet.2006.12.001	journal	January 2007
Control of pathogens and pathobionts by the gut microbiota Kamada, Nobuhiko; Chen, Grace Y.; Inohara, Naohiro Nature Immunology, Vol. 14, Issue 7 https://doi.org/10.1038/ni.2608	journal	June 2013
Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria Wells, Jerry M.; Mercenier, Annick Nature Reviews Microbiology, Vol. 6, Issue 5 https://doi.org/10.1038/nrmicro1840	journal	May 2008
Comparison of Circulating Tumour Cells and Circulating Cell-Free Epstein-Barr Virus DNA in Patients with Nasopharyngeal Carcinoma Undergoing Radiotherapy Vo, Jess Honganh; Nei, Wen Long; Hu, Min Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/s41598-016-0006-3	journal	December 2016
Metabolism of anthocyanins and consequent effects on the gut microbiota Tian, Lingmin; Tan, Yisha; Chen, Guowei Critical Reviews in Food Science and Nutrition, Vol. 59, Issue 6 https://doi.org/10.1080/10408398.2018.1533517	journal	November 2018
Alterations of intestinal barrier and microbiota in chronic kidney disease Sabatino, A.; Regolisti, G.; Brusasco, I. Nephrology Dialysis Transplantation, Vol. 30, Issue 6 https://doi.org/10.1093/ndt/gfu287	journal	September 2014
Breast Milk Jaundice: Effect of Bacteria Present in Breast Milk and Infant Feces Tuzun, Funda; Kumral, Abdullah; Duman, Nuray Journal of Pediatric Gastroenterology & Nutrition, Vol. 56, Issue 3 https://doi.org/10.1097/MPG.0b013e31827a964b	journal	January 2013
Gut microbiota: Role in pathogen colonization, immune responses, and inflammatory disease Pickard, Joseph M.; Zeng, Melody Y.; Caruso, Roberta Immunological Reviews, Vol. 279, Issue 1 https://doi.org/10.1111/imr.12567	journal	August 2017
Safety evaluation of subchronic feeding of nisI transformed Lactobacillus plantarum in Sprague-Dawley rats Liu, Haiyan; He, Xiaoyun; Xu, Wentao Journal of Food Safety, Vol. 38, Issue 2 https://doi.org/10.1111/jfs.12427	journal	December 2017
Genome Sequences of Lactococcus lactis MG1363 (Revised) and NZ9000 and Comparative Physiological Studies Linares, Daniel M.; Kok, Jan; Poolman, Bert Journal of Bacteriology, Vol. 192, Issue 21 https://doi.org/10.1128/JB.00533-10	journal	July 2010
Impairment of spermatogenesis and sperm motility by the high-fat diet-induced dysbiosis of gut microbes Ding, Ning; Zhang, Xin; Zhang, Xue Di Gut, Vol. 69, Issue 9 https://doi.org/10.1136/gutjnl-2019-319127	journal	January 2020
Structural modulation of gut microbiota during alleviation of non-alcoholic fatty liver disease with Gynostemma pentaphyllum in rats Shen, Shu-Hua; Zhong, Ting-Yan; Peng, Cui BMC Complementary Medicine and Therapies, Vol. 20, Issue 1 https://doi.org/10.1186/s12906-020-2835-7	journal	February 2020
Probiotics and microbiota composition Sanders, Mary Ellen BMC Medicine, Vol. 14, Issue 1 https://doi.org/10.1186/s12916-016-0629-z	journal	June 2016
A review on Lactococcus lactis: from food to factory Song, Adelene Ai-Lian; In, Lionel L. A.; Lim, Swee Hua Erin Microbial Cell Factories, Vol. 16, Issue 1 https://doi.org/10.1186/s12934-017-0669-x	journal	April 2017
Microbiome–metabolome reveals the contribution of gut–kidney axis on kidney disease Chen, Yuan-Yuan; Chen, Dan-Qian; Chen, Lin Journal of Translational Medicine, Vol. 17, Issue 1 https://doi.org/10.1186/s12967-018-1756-4	journal	January 2019
Lactobacillus plantarum TW1-1 Alleviates Diethylhexylphthalate-Induced Testicular Damage in Mice by Modulating Gut Microbiota and Decreasing Inflammation Tian, Xiaozhu; Yu, Zhengsheng; Feng, Pengya Frontiers in Cellular and Infection Microbiology, Vol. 9 https://doi.org/10.3389/fcimb.2019.00221	journal	June 2019
Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview Deckers, Marie; Deforce, Dieter; Fraiture, Marie-Alice Foods, Vol. 9, Issue 3 https://doi.org/10.3390/foods9030326	journal	March 2020
Role of the normal gut microbiota Jandhyala, Sai Manasa World Journal of Gastroenterology, Vol. 21, Issue 29 https://doi.org/10.3748/wjg.v21.i29.8787	journal	January 2015

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