Structure of supported DPPC/cholesterol bilayers studied via X-ray reflectivity

Vega, Michael; Lurio, Laurence; Lal, Jyotsana; Karapetrova, Evguenia A.; Gaillard, Elizabeth R.

doi:10.1039/D0CP01834A

Structure of supported DPPC/cholesterol bilayers studied via X-ray reflectivity

Journal Article · Thu Aug 06 00:00:00 EDT 2020 · Physical Chemistry Chemical Physics. PCCP

DOI:https://doi.org/10.1039/D0CP01834A· OSTI ID:1774548

^[1]; Lurio, Laurence ^[1]; Lal, Jyotsana ^[1]; Karapetrova, Evguenia A. ^[2]; ^[1]

Northern Illinois Univ., DeKalb, IL (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)

The electron density profile of bilayers of DPPC/cholesterol mixtures supported on semiconductor grade silicon substrates were studied with the objective of determining how the proximity of a solid interface modifies the phase diagram of mixed bilayers. The bilayers were studiedin situimmersed in waterviasynchrotron X-ray reflectivity (XRR). Measurements were performed as a function of temperature through the main phase transition and cholesterol mole fractions up to 40%. Analysis of XRR yields the bilayer thickness, roughness and leaflet asymmetry. We find that the structure of the pure DPPC bilayers in the gel phase is in agreement with previous X-ray measurements of supported bilayers depositedviavesicle fusion and multilamellar vesicles but show more clearly defined features than measurements made on films formed using Langmuir-Blodget Langmuir-Shaffer (LB) deposition. Examination of bilayer thicknessvs.temperature shows that the melting temperature for supported bilayers is shifted upwards by approximately 4 degrees C relative to multilamellar vesicles and that the melting temperature decreases with increasing cholesterol content up to 20%. For pure DPPC bilayers the leaflets melt in two stages with the distal leaflet melting first. For cholesterol concentrations of 10% and 20% there is no clear indication of separate melting. Finally. for 33% and 40% cholesterol content no clear transition is seen in the bilayer thickness, but an abrupt change in roughness indicates possible microdomain formation in the 40% cholesterol sample.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1774548

Journal Information:: Physical Chemistry Chemical Physics. PCCP, Journal Name: Physical Chemistry Chemical Physics. PCCP Journal Issue: 34 Vol. 22; ISSN 1463-9076

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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