Lactobacillus casei as a biocatalyst for biofuel production

Vinay-Lara, Elena; Wang, Song; Bai, Lina; Phrommao, Ekkarat; Broadbent, Jeff R.; Steele, James L.

doi:10.1007/s10295-016-1797-8

Lactobacillus casei as a biocatalyst for biofuel production

Journal Article · Thu Sep 01 00:00:00 EDT 2016 · Journal of Industrial Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s10295-016-1797-8· OSTI ID:1773515

Vinay-Lara, Elena ^[1]; Wang, Song ^[2]; Bai, Lina ^[3]; Phrommao, Ekkarat ^[1]; Broadbent, Jeff R. ^[4]; Steele, James L. ^[1]

grid.14003.36 0000000121673675 Department of Food Science University of Wisconsin-Madison 127B Babcock Hall, 1605 Linden Drive 53706 Madison WI USA
grid.412243.2 0000000417601136 College of Food Science Northeast Agricultural University Harbin Heilongjiang China
grid.35155.37 0000000417904137 College of Animal Science and Technology Huazhong Agricultural University 430070 Wuhan Hubei China
grid.53857.3c 0000000121858768 Department of Nutrition, Dietetics, and Food Sciences Utah State University 84322-8700 Logan Utah USA

Abstract

Microbial fermentation of sugars from plant biomass to alcohols represents an alternative to petroleum-based fuels. The optimal biocatalyst for such fermentations needs to overcome hurdles such as high concentrations of alcohols and toxic compounds. Lactic acid bacteria, especially lactobacilli, have high innate alcohol tolerance and are remarkably adaptive to harsh environments. This study assessed the potential of five Lactobacillus casei strains as biocatalysts for alcohol production. L. casei 12A was selected based upon its innate alcohol tolerance, high transformation efficiency and ability to utilize plant-derived carbohydrates. A 12A derivative engineered to produce ethanol (L. casei E1) was compared to two other bacterial biocatalysts. Maximal growth rate, maximal optical density and ethanol production were determined under conditions similar to those present during alcohol production from lignocellulosic feedstocks. L. casei E1 exhibited higher innate alcohol tolerance, better growth in the presence of corn stover hydrolysate stressors, and resulted in higher ethanol yields.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1773515

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Issue: 9 Vol. 43; ISSN 1367-5435

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Germany

Language:: English

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