Isolation and structural characterization of sugarcane bagasse lignin after dilute phosphoric acid plus steam explosion pretreatment and its effect on cellulose hydrolysis

Zeng, Jijiao; Tong, Zhaohui; Wang, Letian; Zhu, J. Y.; Ingram, Lonnie

doi:10.1016/j.biortech.2013.12.072

Title: Isolation and structural characterization of sugarcane bagasse lignin after dilute phosphoric acid plus steam explosion pretreatment and its effect on cellulose hydrolysis

Journal Article · Mon Dec 23 00:00:00 EST 2013 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2013.12.072· OSTI ID:1772560

Zeng, Jijiao ^[1]; Tong, Zhaohui ^[1]; Wang, Letian ^[1]; Zhu, J. Y. ^[2]; Ingram, Lonnie ^[1]

Univ. of Florida, Gainesville, FL (United States)
USDA Forest Service, Madison, WI (United States)

The structure of lignin after dilute phosphoric acid plus steam explosion pretreatment process of sugarcane bagasse in a pilot scale and the effect of the lignin extracted by ethanol on subsequent cellulose hydrolysis were investigated. Here, the lignin structural changes caused by pretreatment were identified using advanced nondestructive techniques such as gel permeation chromatography (GPC), quantitative ¹³C, and 2-D nuclear magnetic resonance (NMR). The structural analysis revealed that ethanol extractable lignin preserved basic lignin structure, but had relatively lower amount of β-O-4 linkages, syringyl/guaiacyl units ratio (S/G), p-coumarate/ferulate ratio, and other ending structures. The results also indicated that approximately 8% of mass weight was extracted by pure ethanol. The bagasse after ethanol extraction had an approximate 22% higher glucose yield after enzyme hydrolysis compared to pretreated bagasse without extraction.

Cite

Export

Save

Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of International Affairs (IA)

DOE Contract Number:: PI0000031

OSTI ID:: 1772560

Journal Information:: Bioresource Technology, Vol. 154; ISSN 0960-8524

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

References (31)

Chemical transformations of Populus trichocarpa during dilute acid pretreatment Cao, Shilin; Pu, Yunqiao; Studer, Michael RSC Advances, Vol. 2, Issue 29 https://doi.org/10.1039/c2ra22045h	journal	January 2012
Revisiting the Mechanism of β - O -4 Bond Cleavage during Acidolysis of Lignin. Part 2: Detailed Reaction Mechanism of a Non-Phenolic C ₆ -C ₂ Type Model Compound Yokoyama, Tomoya; Matsumoto, Yuji Journal of Wood Chemistry and Technology, Vol. 30, Issue 3 https://doi.org/10.1080/02773811003675288	journal	August 2010
Optimizing cellulase usage for improved mixing and rheological properties of acid-pretreated sugarcane bagasse Geddes, Claudia C.; Peterson, James J.; Mullinnix, Michael T. Bioresource Technology, Vol. 101, Issue 23 https://doi.org/10.1016/j.biortech.2010.07.040	journal	December 2010
Surface Characterization of Dilute Acid Pretreated Populus deltoides by ToF-SIMS Jung, Seokwon; Foston, Marcus; Sullards, M. Cameron Energy & Fuels, Vol. 24, Issue 2 https://doi.org/10.1021/ef901062p	journal	February 2010
Phenolic acid bridges between polysaccharides and lignin in wheat internodes Iiyama, Kenji; Lam, Thi Bach Tuyet; Stone, Bruce A. Phytochemistry, Vol. 29, Issue 3 https://doi.org/10.1016/0031-9422(90)80009-6	journal	January 1990
Ferulate cross-linking in cell walls isolated from maize cell suspensions Grabber, John H.; Hatfield, Ronald D.; Ralph, John Phytochemistry, Vol. 40, Issue 4, p. 1077-1082 https://doi.org/10.1016/0031-9422(95)00413-2	journal	November 1995
Lignin content in natural Populus variants affects sugar release Studer, M. H.; DeMartini, J. D.; Davis, M. F. Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305 https://doi.org/10.1073/pnas.1009252108	journal	March 2011
Optimizing the saccharification of sugar cane bagasse using dilute phosphoric acid followed by fungal cellulases Geddes, C. C.; Peterson, J. J.; Roslander, C. Bioresource Technology, Vol. 101, Issue 6, p. 1851-1857 https://doi.org/10.1016/j.biortech.2009.09.070	journal	March 2010
Organosolv Ethanol Lignin from Hybrid Poplar as a Radical Scavenger: Relationship between Lignin Structure, Extraction Conditions, and Antioxidant Activity Pan, Xuejun; Kadla, John F.; Ehara, Katsunobu Journal of Agricultural and Food Chemistry, Vol. 54, Issue 16 https://doi.org/10.1021/jf0605392	journal	August 2006
Lignin-ferulate cross-links in grasses: active incorporation of ferulate polysaccharide esters into ryegrass lignins Ralph, John; Grabber, John H.; Hatfield, Ronald D. Carbohydrate Research, Vol. 275, Issue 1, p. 167-178 https://doi.org/10.1016/0008-6215(95)00237-N	journal	September 1995
Adsorption of Cellulase on Cellulolytic Enzyme Lignin from Lodgepole Pine Tu, Maobing; Pan, Xuejun; Saddler, Jack N. Journal of Agricultural and Food Chemistry, Vol. 57, Issue 17 https://doi.org/10.1021/jf901031m	journal	September 2009
Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment Chundawat, Shishir P. S.; Donohoe, Bryon S.; da Costa Sousa, Leonardo Energy & Environmental Science, Vol. 4, Issue 3, 973 https://doi.org/10.1039/c0ee00574f	journal	January 2011
Quantification of Wheat Straw Lignin Structure by Comprehensive NMR Analysis Zeng, Jijiao; Helms, Gregory L.; Gao, Xin Journal of Agricultural and Food Chemistry, Vol. 61, Issue 46 https://doi.org/10.1021/jf4030486	journal	November 2013
Structural Characterization and Comparison of Switchgrass Ball-milled Lignin Before and After Dilute Acid Pretreatment Samuel, Reichel; Pu, Yunqiao; Raman, Babu Applied Biochemistry and Biotechnology, Vol. 162, Issue 1 https://doi.org/10.1007/s12010-009-8749-y	journal	August 2009
Lignin in straw of herbaceous crops Buranov, Anvar U.; Mazza, G. Industrial Crops and Products, Vol. 28, Issue 3 https://doi.org/10.1016/j.indcrop.2008.03.008	journal	November 2008
Characterization of lignin derived from water-only flowthrough pretreatment of Miscanthus Laskar, Dhrubojyoti D.; Zeng, Jijiao; Yan, Lishi Industrial Crops and Products, Vol. 50 https://doi.org/10.1016/j.indcrop.2013.08.002	journal	October 2013
Chemical and Spatial Differentiation of Syringyl and Guaiacyl Lignins in Poplar Wood via Time-of-Flight Secondary Ion Mass Spectrometry Zhou, Chuanzhen; Li, Quanzi; Chiang, Vincent L. Analytical Chemistry, Vol. 83, Issue 18 https://doi.org/10.1021/ac200903y	journal	September 2011
Simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant Escherichia coli strain MM160 Geddes, C. C.; Mullinnix, M. T.; Nieves, I. U. Bioresource Technology, Vol. 102, Issue 3, p. 2702-2711 https://doi.org/10.1016/j.biortech.2010.10.143	journal	February 2011
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production Himmel, M. E.; Ding, S.-Y.; Johnson, D. K. Science, Vol. 315, Issue 5813, p. 804-807 https://doi.org/10.1126/science.1137016	journal	February 2007
The Path Forward for Biofuels and Biomaterials Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H. Science, Vol. 311, Issue 5760, p. 484-489 https://doi.org/10.1126/science.1114736	journal	January 2006
Deposition of Lignin Droplets Produced During Dilute Acid Pretreatment of Maize Stems Retards Enzymatic Hydrolysis of Cellulose Selig, M. J.; Viamajala, S.; Decker, S. R. Biotechnology Progress, Vol. 23, Issue 6 https://doi.org/10.1021/bp0702018	journal	December 2007
Organosolv pretreatment of lignocellulosic biomass for enzymatic hydrolysis Zhao, Xuebing; Cheng, Keke; Liu, Dehua Applied Microbiology and Biotechnology, Vol. 82, Issue 5 https://doi.org/10.1007/s00253-009-1883-1	journal	February 2009
Biomass Fractionation for the Biorefinery: Heteronuclear Multiple Quantum Coherence–Nuclear Magnetic Resonance Investigation of Lignin Isolated from Solvent Fractionation of Switchgrass Bozell, Joseph J.; O'Lenick, C. J.; Warwick, Stacy Journal of Agricultural and Food Chemistry, Vol. 59, Issue 17 https://doi.org/10.1021/jf201850b	journal	September 2011
Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d ₆ Kim, Hoon; Ralph, John; Akiyama, Takuya BioEnergy Research, Vol. 1, Issue 1, p. 56-66 https://doi.org/10.1007/s12155-008-9004-z	journal	March 2008
Quantitative Structures and Thermal Properties of Birch Lignins after Ionic Liquid Pretreatment Wen, Jia-Long; Sun, Shao-Long; Xue, Bai-Liang Journal of Agricultural and Food Chemistry, Vol. 61, Issue 3 https://doi.org/10.1021/jf3051939	journal	January 2013
Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption Rahikainen, Jenni L.; Martin-Sampedro, Raquel; Heikkinen, Harri Bioresource Technology, Vol. 133 https://doi.org/10.1016/j.biortech.2013.01.075	journal	April 2013
Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals Chundawat, Shishir P. S.; Beckham, Gregg T.; Himmel, Michael E. Annual Review of Chemical and Biomolecular Engineering, Vol. 2, Issue 1, p. 121-145 https://doi.org/10.1146/annurev-chembioeng-061010-114205	journal	July 2011
The isolation, characterization and effect of lignin isolated from steam pretreated Douglas-fir on the enzymatic hydrolysis of cellulose Nakagame, Seiji; Chandra, Richard P.; Kadla, John F. Bioresource Technology, Vol. 102, Issue 6 https://doi.org/10.1016/j.biortech.2010.12.082	journal	March 2011
Lignosulfonate To Enhance Enzymatic Saccharification of Lignocelluloses: Role of Molecular Weight and Substrate Lignin Zhou, Haifeng; Lou, Hongming; Yang, Dongjie Industrial & Engineering Chemistry Research, Vol. 52, Issue 25 https://doi.org/10.1021/ie401085k	journal	June 2013
Structural Characterization of Wheat Straw Lignin as Revealed by Analytical Pyrolysis, 2D-NMR, and Reductive Cleavage Methods del Río, José C.; Rencoret, Jorge; Prinsen, Pepijn Journal of Agricultural and Food Chemistry, Vol. 60, Issue 23 https://doi.org/10.1021/jf301002n	journal	December 2011
Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine Zhu, J. Y.; Pan, X. J.; Wang, G. S. Bioresource Technology, Vol. 100, Issue 8 https://doi.org/10.1016/j.biortech.2008.10.057	journal	April 2009

Similar Records

Characteristics of Lignin Fractions from Dilute Acid Pretreated Switchgrass and Their Effect on Cellobiohydrolase from Trichoderma longibrachiatum

Journal Article · Tue Feb 06 00:00:00 EST 2018 · Frontiers in Energy Research · OSTI ID:1772560

Yao, Lan; Yang, Haitao; Yoo, Chang Geun; +4 more

Comparison of autohydrolysis and ionic liquid 1-butyl-3-methylimidazolium acetate pretreatment to enhance enzymatic hydrolysis of sugarcane bagasse

Journal Article · Wed Nov 02 00:00:00 EDT 2016 · Bioresource Technology · OSTI ID:1772560

Hashim, Muzna; Sun, Qining; Tao, Jingming; +3 more

Physico-chemical properties of lignin fractions from acid pretreated corn stover and their effects on enzymatic hydrolysis of microcrystalline cellulose

Journal Article · Mon May 11 00:00:00 EDT 2020 · BioResources · OSTI ID:1772560

Yang, Haitao; Zhang, Yuhang; Yoo, Chang Geun; +4 more

Related Subjects

Lignin structure
Soluble lignin
Nuclear magnetic resonance (NMR)
Cellulose hydrolysis

Title: Isolation and structural characterization of sugarcane bagasse lignin after dilute phosphoric acid plus steam explosion pretreatment and its effect on cellulose hydrolysis

Citation Formats

References (31)

Similar Records

Related Subjects