Fusion constructs as protein over-expression vectors

Melis, Anastasios; Formighieri, Cinzia

Fusion constructs as protein over-expression vectors

Patent · 29 December 2020

OSTI ID:1771762

Melis, Anastasios; Formighieri, Cinzia

This invention provides compositions and methods for providing high product yield of transgenes expressed in cyanobacteria and microalgae.

Research Organization:: Univ. of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AR0000204

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 10,876,124

Application Number:: 15/739,101

OSTI ID:: 1771762

Country of Publication:: United States

Language:: English

References (11)

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Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants Formighieri, Cinzia; Melis, Anastasios Planta, Vol. 240, Issue 2 https://doi.org/10.1007/s00425-014-2080-8	journal	May 2014
Heterologous Expression of the Mevalonic Acid Pathway in Cyanobacteria Enhances Endogenous Carbon Partitioning to Isoprene Bentley, Fiona K.; Zurbriggen, Andreas; Melis, Anastasios Molecular Plant, Vol. 7, Issue 1 https://doi.org/10.1093/mp/sst134	journal	January 2014
Paradigm of Monoterpene (β-phellandrene) Hydrocarbons Production via Photosynthesis in Cyanobacteria Bentley, Fiona K.; García-Cerdán, Jose Gines; Chen, Hsu-Ching BioEnergy Research, Vol. 6, Issue 3 https://doi.org/10.1007/s12155-013-9325-4	journal	March 2013
The Firm In Search Of Its Nature Grandori, Anna European Management Review, Vol. 16, Issue 1 https://doi.org/10.1111/emre.12178	journal	May 2018
Automated overexpression and isotopic labelling of biologically active oncoproteins in the cyanobacterium Anabaena sp. PCC 7120 Desplancq, Dominique; Rinaldi, Anne-Sophie; Hörzer, Helen Biotechnology and Applied Biochemistry, Vol. 51, Issue 1 https://doi.org/10.1042/BA20070276	journal	September 2008
Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast Muto, Machiko; Henry, Ryan E.; Mayfield, Stephen P. BMC Biotechnology, Vol. 9, Issue 1 https://doi.org/10.1186/1472-6750-9-26	journal	March 2009
Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size Kirst, Henning; Formighieri, Cinzia; Melis, Anastasios Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 10 https://doi.org/10.1016/j.bbabio.2014.07.009	journal	October 2014
Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria Zhou, Jie; Zhang, Haifeng; Meng, Hengkai Scientific Reports, Vol. 4, Issue 1 https://doi.org/10.1038/srep04500	journal	March 2014
Strong and regulated promoters in the cyanobacterium Anabaena PCC 7120 Elhai, Jeff FEMS Microbiology Letters, Vol. 114, Issue 2 https://doi.org/10.1111/j.1574-6968.1993.tb06570.x	journal	December 1993
Synthetic Biology Toolbox for Controlling Gene Expression in the Cyanobacterium Synechococcus sp. strain PCC 7002 Markley, Andrew L.; Begemann, Matthew B.; Clarke, Ryan E. ACS Synthetic Biology, Vol. 4, Issue 5 https://doi.org/10.1021/sb500260k	journal	September 2014

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