Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium

Sawisit, Apichai; Jantama, Kaemwich; Zheng, Huabao; Yomano, Lorraine P.; York, Sean W.; Shanmugam, Keelnatham T.; Ingram, Lonnie O.

doi:10.1016/j.biortech.2015.06.108

Title: Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium

Journal Article · Thu Jun 25 00:00:00 EDT 2015 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2015.06.108· OSTI ID:1771326

Sawisit, Apichai ^[1]; Jantama, Kaemwich ^[1]; Zheng, Huabao ^[2]; Yomano, Lorraine P. ^[2]; York, Sean W. ^[2]; Shanmugam, Keelnatham T. ^[2]; Ingram, Lonnie O. ^[2]

Suranaree Univ. of Technology, Nakhon Ratchasima (Thailand)
Univ. of Florida, Gainesville, FL (United States)

Escherichia coli KJ122 was engineered to produce succinate from glucose using the wild type GalP for glucose uptake instead of the native phosphotransferase system (ptsI mutation). This strain now ferments 10% xylose poorly. Mutants were selected by serial transfers in AM1 mineral salts medium with 10% xylose. Clones from this population all exhibited a similar improvement, co-fermentation of an equal mixture of xylose and glucose. One of these, AS1600a, produced 84.26 ± 1.37 g/L succinate, equivalent to that produced by the parent (KJ122) from 10% glucose (85.46 ± 1.78 g/L). AS1600a was sequenced and found to contain a mutation in galactose permease (GalP, G236D). This mutation was shown to be responsible for the improvement in fermentation using KJΔgalP as the host and expression vectors with native galP and with mutant galP*. Strain AS1600a and KJΔgalP(pLOI5746; galP*) also co-fermented a mixture of glucose, xylose, arabinose, and galactose in sugarcane bagasse hydrolysate using mineral salts medium.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of International Affairs (IA); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; U.S. Department of Agriculture; Thailand Research Fund (TRF)

Grant/Contract Number:: PI0000031; PHD/0135/2553

OSTI ID:: 1771326

Alternate ID(s):: OSTI ID: 1250363; OSTI ID: 1661478

Journal Information:: Bioresource Technology, Vol. 193; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 26 works

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ATP-Based Ratio Regulation of Glucose and Xylose Improved Succinate Production Zhang, Fengyu; Li, Jiaojiao; Liu, Huaiwei PLOS ONE, Vol. 11, Issue 6 https://doi.org/10.1371/journal.pone.0157775	journal	June 2016
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Title: Mutation in galP improved fermentation of mixed sugars to succinate using engineered Escherichia coli AS1600a and AM1 mineral salts medium

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