Accurate determination of genotypic variance of cell wall characteristics of a&nbsp;Populus trichocarpa&nbsp;pedigree using high-throughput pyrolysis-molecular beam mass spectrometry

Harman-Ware, Anne E.; Macaya-Sanz, David; Abeyratne, Chanaka Roshan; Doeppke, Crissa; Haiby, Kathleen; Tuskan, Gerald A.; Stanton, Brian; DiFazio, Stephen P.; Davis, Mark F.

doi:10.1186/s13068-021-01908-y

Accurate determination of genotypic variance of cell wall characteristics of a Populus trichocarpa pedigree using high-throughput pyrolysis-molecular beam mass spectrometry

Journal Article · Sat Mar 06 00:00:00 EST 2021 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-021-01908-y· OSTI ID:1769477

; Macaya-Sanz, David; Abeyratne, Chanaka Roshan; Doeppke, Crissa; Haiby, Kathleen; Tuskan, Gerald A.; Stanton, Brian; DiFazio, Stephen P.; Davis, Mark F.

Abstract Background

Pyrolysis-molecular beam mass spectrometry (py-MBMS) analysis of a pedigree of Populus trichocarpa was performed to study the phenotypic plasticity and heritability of lignin content and lignin monomer composition. Instrumental and microspatial environmental variability were observed in the spectral features and corrected to reveal underlying genetic variance of biomass composition.

Results

Lignin-derived ions (including m/z 124, 154, 168, 194, 210 and others) were highly impacted by microspatial environmental variation which demonstrates phenotypic plasticity of lignin composition in Populus trichocarpa biomass. Broad-sense heritability of lignin composition after correcting for microspatial and instrumental variation was determined to be H ² = 0.56 based on py-MBMS ions known to derive from lignin. Heritability of lignin monomeric syringyl/guaiacyl ratio ( S / G ) was H ² = 0.81. Broad-sense heritability was also high (up to H ² = 0.79) for ions derived from other components of the biomass including phenolics (e.g., salicylates) and C5 sugars (e.g., xylose). Lignin and phenolic ion abundances were primarily driven by maternal effects, and paternal effects were either similar or stronger for the most heritable carbohydrate-derived ions.

Conclusions

We have shown that many biopolymer-derived ions from py-MBMS show substantial phenotypic plasticity in response to microenvironmental variation in plantations. Nevertheless, broad-sense heritability for biomass composition can be quite high after correcting for spatial environmental variation. This work outlines the importance in accounting for instrumental and microspatial environmental variation in biomass composition data for applications in heritability measurements and genomic selection for breeding poplar for renewable fuels and materials.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: U.S. Department of Transportation (DOT); USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC36-08GO28308

Other Award/Contract Number:: Western Sun Grant program (T0013A)

OSTI ID:: 1769477

Alternate ID(s):: OSTI ID: 1772442

Report Number(s):: NREL-JA--2800-74879; 59; PII: 1908

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 14; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Accurate determination of genotypic variance of cell wall characteristics of a Populus trichocarpa pedigree using high-throughput pyrolysis-molecular beam mass spectrometry

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