Understanding the adsorption and potential tear film stability properties of recombinant human lubricin and bovine submaxillary mucins in an in vitro tear film model

Rabiah, Noelle I.; Sato, Yasunori; Kannan, Aadithya; Kress, Wolfgang; Straube, Frank; Fuller, Gerald G.

doi:10.1016/j.colsurfb.2020.111257

Understanding the adsorption and potential tear film stability properties of recombinant human lubricin and bovine submaxillary mucins in an in vitro tear film model

Journal Article · Sat Jul 18 00:00:00 EDT 2020 · Colloids and Surfaces. B, Biointerfaces

DOI:https://doi.org/10.1016/j.colsurfb.2020.111257· OSTI ID:1769032

^[1]; Sato, Yasunori ^[2]; ^[1]; Kress, Wolfgang ^[3]; Straube, Frank ^[3]; ^[1]

Stanford Univ., CA (United States)
Nagaoka Univ. of Technology (Japan)
Novartis Pharma AG, Basel (Switzerland)

The wetting and adsorption properties for two glycoproteins, recombinant human lubricin and bovine submaxillary mucins (BSM) were evaluated on hydrophilic and hydrophobic glass dome surfaces in a simplified in vitro tear film model. Herein, we show that both recombinant human lubricin (rh-lubricin) and BSM solutions render surfaces hydrophilic and when the fluid films reach 500 nm or less, the fluids resist evaporation-driven breakup through a volumetric flux across the surface, which we believe is due to evaporation-driven solutocapillary flows. rh-Lubricin was able to maintain a wet film without spontaneous breakup for longer periods of time than BSM at lower concentrations, which we attribute to differences in adsorption properties, measured by QCM-D, that result from surface charge and structural differences (confirmed by zeta potential, DLS, and SAXS measurements).

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1769032

Alternate ID(s):: OSTI ID: 1778402

Journal Information:: Colloids and Surfaces. B, Biointerfaces, Journal Name: Colloids and Surfaces. B, Biointerfaces Vol. 195; ISSN 0927-7765

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Understanding the adsorption and potential tear film stability properties of recombinant human lubricin and bovine submaxillary mucins in an in vitro tear film model

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