Removing chiral contamination of lactate solutions by selective metabolism of the D-enantiomer

Chauliac, Diane; Pullammanappallil, P. C.; Ingram, L. O.; Shanmugam, K. T.

doi:10.1007/s10529-015-1924-z

Removing chiral contamination of lactate solutions by selective metabolism of the D-enantiomer

Journal Article · Fri Aug 14 00:00:00 EDT 2015 · Biotechnology Letters

DOI:https://doi.org/10.1007/s10529-015-1924-z· OSTI ID:1766806

Chauliac, Diane ^[1]; Pullammanappallil, P. C. ^[2]; Ingram, L. O. ^[2]; Shanmugam, K. T. ^[2]

Univ. of Florida, Gainesville, FL (United States); University of Florida
Univ. of Florida, Gainesville, FL (United States)

A bio-based process is appealing for purification of L-lactic acid, the major enantiomer of polylactic acid syrup, generated by thermochemical processes at the end of life of PLA-based plastics, from its chiral impurity, d-lactic acid, before reuse. Polylactic acid (PLA), a renewable alternative to petroleum-derived plastics, contains a mixture of l- and d-lactic acid (LA) isomers with the l-isomer dominating (up to 95 %). A novel bio-based process was developed to produce chirally pure l-LA from syrup produced during recycling of PLA-plastics. This process utilizes an engineered Escherichia coli (strain DC1001) containing novel gene deletions (lld, ykg) that eliminated the oxidative metabolism of L-lactate, leaving the membrane-bound d-lactate dehydrogenases to selectively metabolize the d-isomer. Strain DC1001 removed 8.7 g d-lactate l^–1 from a PLA-syrup containing 135 g total lactic acid l^–1 in 24 h. Average rates of removal of D-lactic acid were 0.25 g D-lactate h^–1 (g cell dry weight)^–1 and 0.36 g D-lactate l^–1 h^–1. Bio-based purification of PLA-syrup utilizing E. coli strain DC1001 is an attractive process step during recycling of PLA-plastics. Here, this selective oxidation process can also be used to remove chiral contamination of L-lactate in medical applications.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE Office of International Affairs (IA)

Grant/Contract Number:: PI0000031

OSTI ID:: 1766806

Alternate ID(s):: OSTI ID: 1771310

Journal Information:: Biotechnology Letters, Journal Name: Biotechnology Letters Journal Issue: 12 Vol. 37; ISSN 0141-5492

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Removing chiral contamination of lactate solutions by selective metabolism of the D-enantiomer

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