KS4A-Omics1.0_FspDS682

Dataset · Tue Apr 20 00:00:00 EDT 2021

DOI:https://doi.org/10.25584/KSOmicsFspDS682/1766303· OSTI ID:1766303

Anderson, Lindsey N  ^[1]; Bhattacharjee, Arunima  ^[1]; Richardson, Jocelyn R  ^[2]; Bramer, Lisa M  ^[1]; Qafoku, Odeta  ^[3]; Zhu, Zihua  ^[3]; Bowden, Mark E  ^[3]; Engelhard, Mark H  ^[3]; Nelson, William C  ^[1]; Hofmockel, Kirsten S  ^[1]; Jansson, Janet K  ^[1]; Anderton, Christopher R  ^[1]

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1. Pacific Northwest National Laboratory, Biological Sciences Division (EBSD), PNNL Soil Microbiome SFA Project
2. SLAC National Accelerator Laboratory
3. Pacific Northwest National Laboratory, EMSL

Contributors:

Other:

McDermott, Jason  [1]; Smith, Ian  [2]; Hofmockel, Michael  [2]

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1. Pacific Northwest National Laboratory, Biological Sciences Division (EBSD), PNNL Soil Microbiome SFA Project
2. Pacific Northwest National Laboratory DataHub (https://data.pnnl.gov/), pnnl2

Soil fungi facilitate the translocation of inorganic nutrients from soil minerals to other microorganisms and plants. This ability is particularly advantageous in impoverished soils, because fungal mycelial networks can bridge otherwise spatially disconnected and inaccessible nutrient hotspots. However, the molecular mechanisms underlying fungal mineral weathering and transport through soil remains poorly understood. Here, we addressed this knowledge gap by directly visualizing nutrient acquisition and transport through fungal hyphae in a mineral doped soil micromodel using a multimodal imaging approach. Here, we observed how a representative of common saprotrophic soil fungi, Fusarium sp. DS 682, exhibited a mechanosensory response (thigmotropism) around obstacles and through pore spaces (~12 μm) in the presence of minerals.This study establishes the significance of fungal biology and nutrient translocation mechanisms in maintaining fungal growth under water and nutrient limitations in a soil-like microenvironment, using a high-throughput multi-omic analysis approach. Data package KS4A-Omics.1.0_FspDS682 (Publication: Fungal Mineral Weathering Mechanisms Revealed Through Direct Molecular Visualization) contents reported here are the first version (1.0) and contain pre- and post-processed data using high throughput data capture technologies for multi-omic analysis and integration, this data package contains raw and post-processed experimental data for X-Ray Absorption Near Edge Structure Spectroscopy (XANES/XRF), Optical Microscopy, Proteomics, Scanning Electron Microscope (SEM), Time-of-Flight Secondary Ion Mass Spectroscopy (ToF-SIMS), X-Ray Diffraction Spectroscopy (XRD) files, and X- Ray Photoelectron Spectroscopy (XPS) using EMSL capabilities. This data package DOI contains a comprehensive collection of high-throughput multi-omics data and process metadata catalog. Support files include additional data download contents “Read Me” with dataset descriptor information and data source method application ontologies (see data dictionary section). Reported data download content is structured for compliance with reported guidelines provided by community standard initiatives and publisher stakeholder policies supporting FAIR data principles.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: SC-23
• DOE Contract Number: AC05-76RLO1830; AC02-76SF00515
• OSTI ID: 1766303
• Report Number(s): 13432
• Availability: [e-mail]
• Country of Publication: United States
• Language: EN

Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
47 OTHER INSTRUMENTATION
Fusarium sp. DS 682
Microscopy
Spectroscopy
Mass Spectrometry
Multi-Omics
Data Package DOI