SINGLE: Atomic-resolution structure identification of nanocrystals by graphene liquid cell EM

Reboul, Cyril F.; Heo, Junyoung; Machello, Chiara; Kiesewetter, Simon; Kim, Byung Hyo; Kim, Sungin; Elmlund, Dominika; Ercius, Peter; Park, Jungwon; Elmlund, Hans

doi:10.1126/sciadv.abe6679

SINGLE: Atomic-resolution structure identification of nanocrystals by graphene liquid cell EM

Journal Article · Thu Jan 28 23:00:00 EST 2021 · Science Advances

DOI:https://doi.org/10.1126/sciadv.abe6679· OSTI ID:1763794

^[1]; ^[2]; Machello, Chiara ^[1]; Kiesewetter, Simon ^[1]; ^[3]; ^[2]; Elmlund, Dominika ^[1]; ^[4]; ^[2]; ^[1]

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Melbourne, Victoria, Australia.; Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Melbourne, Victoria, Australia.
Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul 08826, South Korea.; School of Chemical and Biological Engineering, Institute of Chemical Process, Seoul National University, Seoul 08826, South Korea.
Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul 08826, South Korea.; School of Chemical and Biological Engineering, Institute of Chemical Process, Seoul National University, Seoul 08826, South Korea.; Department of Organic Materials and Fiber Engineering, Soongsil University, Seoul 06978, South Korea.
National Center for Electron Microscopy, Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Analysis of the three-dimensional (3D) structures of nanocrystals with solution-phase transmission electron microscopy is beginning to reveal their unique physiochemical properties. We developed a “one-particle Brownian 3D reconstruction method” based on imaging of ensembles of colloidal nanocrystals using graphene liquid cell electron microscopy. Projection images of differently rotated nanocrystals are acquired using a direct electron detector with high temporal (<2.5 ms) resolution and analyzed to obtain an ensemble of 3D reconstructions. Here, we introduce computational methods required for successful atomic-resolution 3D reconstruction: (i) tracking of the individual particles throughout the time series, (ii) subtraction of the interfering background of the graphene liquid cell, (iii) identification and rejection of low-quality images, and (iv) tailored strategies for 2D/3D alignment and averaging that differ from those used in biological cryo–electron microscopy. Our developments are made available through the open-source software package SINGLE.

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Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division; Institute for Basic Science; National Research Foundation of Korea (NRF); Ministry of Trade, Industry & Energy (MOTIE); Korea Semiconductor Research Consortium (KSRC); Samsung Science and Technology Foundation (SSTF); Australian Research Council (ARC); Australian National Health and Medical Research Council

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1763794

Alternate ID(s):: OSTI ID: 1769332

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 5 Vol. 7; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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