Biomineralization of Cu2S Nanoparticles by Geobacter sulfurreducens

Kimber, Richard L.; Bagshaw, Heath; Smith, Kurt; Buchanan, Dawn M.; Coker, Victoria S.; Cavet, Jennifer S.; Lloyd, Jonathan R.

doi:10.1128/aem.00967-20

Biomineralization of Cu₂S Nanoparticles by Geobacter sulfurreducens

Journal Article · Tue Sep 01 00:00:00 EDT 2020 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/aem.00967-20· OSTI ID:1756101

^[1]; Bagshaw, Heath ^[1]; Smith, Kurt ^[1]; Buchanan, Dawn M. ^[1]; Coker, Victoria S. ^[1]; Cavet, Jennifer S. ^[1]; Lloyd, Jonathan R. ^[1]

Univ. of Manchester (United Kingdom)

Biomineralization of Cu has been shown to control contaminant dynamics and transport in soils. However, very little is known about the role that subsurface microorganisms may play in the biogeochemical cycling of Cu. In this study, we investigate the bioreduction of Cu(II) by the subsurface metal-reducing bacterium Geobacter sulfurreducens. Rapid removal of Cu from solution was observed in cell suspensions of G. sulfurreducens when Cu(II) was supplied, while transmission electron microscopy (TEM) analyses showed the formation of electron-dense nanoparticles associated with the cell surface. Energy-dispersive X-ray spectroscopy (EDX) point analysis and EDX spectrum image maps revealed that the nanoparticles are rich in both Cu and S. This finding was confirmed by X-ray absorption near-edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) analyses, which identified the nanoparticles as Cu₂S. Biomineralization of Cu_xS nanoparticles in soils has been reported to enhance the colloidal transport of a number of contaminants, including Pb, Cd, and Hg. However, formation of these Cu_xS nanoparticles has only been observed under sulfate-reducing conditions and could not be repeated using isolates of implicated organisms. As G. sulfurreducens is unable to respire sulfate, and no reducible sulfur was supplied to the cells, these data suggest a novel mechanism for the biomineralization of Cu₂S under anoxic conditions. The implications of these findings for the biogeochemical cycling of Cu and other metals as well as the green production of Cu catalysts are discussed.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Biotechnology and Biological Sciences Research Council (BBSRC); North American Electric Reliability Corporation (NERC); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1756101

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 18 Vol. 86; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Biomineralization of Cu₂S Nanoparticles by Geobacter sulfurreducens