Chimeric cellobiohydrolase I expression, activity, and biochemical properties in three oleaginous yeast

Alahuhta, Markus; Xu, Qi; Knoshaug, Eric P.; Wang, Wei; Wei, Hui; Amore, Antonella; Baker, John O.; Vander Wall, Todd; Himmel, Michael E.; Zhang, Min

doi:10.1186/s13068-020-01856-z

Chimeric cellobiohydrolase I expression, activity, and biochemical properties in three oleaginous yeast

Journal Article · Tue Jan 05 23:00:00 EST 2021 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-020-01856-z· OSTI ID:1756003

; Xu, Qi; Knoshaug, Eric P.; Wang, Wei; Wei, Hui; Amore, Antonella; Baker, John O.; Vander Wall, Todd; Himmel, Michael E.; Zhang, Min

Consolidated bioprocessing using oleaginous yeast is a promising modality for the economic conversion of plant biomass to fuels and chemicals. However, yeast are not known to produce effective biomass degrading enzymes naturally and this trait is essential for efficient consolidated bioprocessing. We expressed a chimeric cellobiohydrolase I gene in three different oleaginous, industrially relevant yeast: Yarrowia lipolytica, Lipomyces starkeyi, and Saccharomyces cerevisiae to study the biochemical and catalytic properties and biomass deconstruction potential of these recombinant enzymes. Our results showed differences in glycosylation, surface charge, thermal and proteolytic stability, and efficacy of biomass digestion. L. starkeyi was shown to be an inferior active cellulase producer compared to both the Y. lipolytica and S. cerevisiae enzymes, whereas the cellulase expressed in S. cerevisiae displayed the lowest activity against dilute-acid-pretreated corn stover. Comparatively, the chimeric cellobiohydrolase I enzyme expressed in Y. lipolytica was found to have a lower extent of glycosylation, better protease stability, and higher activity against dilute-acid-pretreated corn stover.

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Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1756003

Alternate ID(s):: OSTI ID: 1762468

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 14; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Chimeric cellobiohydrolase I expression, activity, and biochemical properties in three oleaginous yeast

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Chimeric cellobiohydrolase I expression, activity, and biochemical properties in three oleaginous yeast

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